Lidocaine represses the malignant behavior of lung carcinoma cells via the circ_PDZD8/miR-516b-5p/GOLT1A axis

Lung carcinoma is the most prevalent malignancy in adults. Lidocaine (Lido) has been confirmed to exert an anti-tumor role in many human cancers. However, the role and underlying mechanism of Lido in lung carcinoma remain poorly understood. Cell proliferation ability, migration, invasion, and apoptosis were measured by Colony formation, 5-ethynyl-2'- deoxyuridine (EdU), Cell Counting Kit-8 (CCK-8), transwell, and flow cytometry assays. Circ_PDZD8, microRNA-516b-5p (miR-516b-5p), and Golgi transport 1A (GOLT1A) levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Protein levels of proliferating cell nuclear antigen (PCNA) and GOLT1A were examined by western blot assay. The binding relationship between miR-516b-5p and circ_PDZD8 or GOLT1A was predicted by circular RNA Interactome or Starbase 3.0 and then verified by a dual-luciferase reporter assay. The biological roles of circ_PDZD8 and Lido on lung carcinoma cell growth were examined by the xenograft tumor model in vivo. Lido suppressed proliferation, migration, invasion, and induced apoptosis in lung carcinoma cells. Circ_PDZD8 and GOLT1A were increased, miR-516b-5p was decreased in lung carcinoma tissues and cell lines. Their expression presented the opposite trend in Lido-triggered lung carcinoma cells. Circ_PDZD8 might overturn the repression of Lido on cell growth ability and metastasis in this tumor. Mechanically, circ_PDZD8 might regulate GOLT1A expression by sponging miR-516b-5p. Circ_PDZD8 weakened the anti-lung carcinoma effect of Lido in vivo. Circ_PDZD8 might mitigate the inhibitory effect of Lido on tumor cell malignancy by modulating the miR-516b-5p/GOLT1A axis, providing a novel insight for lung carcinoma treatment

Dynamic Pricing of Tropical Fruits in Hainan Based on Internet of Things Technology

In recent years, the Internet of Things (IoT) technology has been widely used in the production and sales of tropical fruits, with strong practicability and wide application prospects. The tropical fruit dynamic pricing model based on the IoT technology can promote the healthy development of the tropical fruit industry in Hainan and ensure the income of fruit farmers. Based on IoT technology, the quality grade of tropical fruits in Hainan is obtained. According to the dynamic pricing strategy of revenue management, a dynamic pricing model based on the quality of tropical fruits and a dynamic pricing model based on consumer segmentation are established to study the dynamic pricing problem under the condition of maximum profit for tropical fruit sellers. The research results show that for different fruit quality and consumer groups, different pricing models are required for pricing, in order to get the maximum profit from tropical fruit sales. Sellers must flexibly adopt different dynamic pricing models to price tropical fruits to enhance the competitiveness of the tropical fruit industry

Absorption and Bio-Transformation of Selenium Nanoparticles by Wheat Seedlings (Triticum aestivum L.)

Elemental selenium is one of the dominant selenium species in soil, but the mechanism of its uptake by plants is still unclear. In this study, nanoparticles of elemental selenium (SeNPs) with different sizes were prepared, and their uptake and transformation in wheat (Triticum aestivum L.) were analyzed in hydroponic experiments by HPLC-ICP-MS. We found that the SeNPs can be absorbed by wheat seedlings, and the process is energy independent. The addition of aquaporins inhibitor caused 92.5 and 93.4% inhibition of chemosynthesized SeNPs (CheSeNPs) and biosynthesized SeNPs (BioSeNPs) absorption by wheat roots, respectively. The 40 nm SeNPs uptake by wheat roots was 1.8-fold and 2.2-fold higher than that of 140 and 240 nm, respectively. The rate of SeNPs uptake in wheat was much slower than that of selenite [Se (IV)], and CheSeNPs were more efficiently absorbed than BioSeNPs. The SeNPs were rapidly oxidized to Se (IV) and converted to organic forms [selenocystine (SeCys2), se-methyl-selenocysteine (MeSeCys), and selenomethionine (SeMet)] after they were absorbed by wheat roots. Additionally, we demonstrated that the aquaporin function in some way is related to the absorption of SeNPs. The particle size and synthesis method of the SeNPs affected their uptake rates by plants. Taken together, our results provide a deep understanding of the SeNPs uptake mechanism in plants

Photodegradation of glyphosate in water and stimulation of by-products on algae growth

Glyphosate is the most widely used herbicide in global agricultural cultivation. However, little is known about the environmental risks associated with its migration and transformation. We conducted light irradiation experiments to study the dynamics and mechanism of photodegradation of glyphosate in ditches, ponds and lakes, and evaluated the effect of glyphosate photodegradation on algae growth through algae culture experiments. Our results showed that glyphosate in ditches, ponds and lakes could undergo photochemical degradation under sunlight irradiation with the production of phosphate, and the photodegradation rate of glyphosate in ditches could reach 86% after 96 h under sunlight irradiation. Hydroxyl radicals (•OH) was the main reactive oxygen species (ROS) for glyphosate photodegradation, and its steady-state concentrations in ditches, ponds and lakes were 6.22 × 10−17, 4.73 × 10−17, and 4.90 × 10−17 M. The fluorescence emission-excitation matrix (EEM) and other technologies further indicated that the humus components in dissolved organic matter (DOM) and nitrite were the main photosensitive substances producing •OH. In addition, the phosphate generated by glyphosate photodegradation could greatly promote the growth of Microcystis aeruginosa, thereby increasing the risk of eutrophication. Thus, glyphosate should be scientifically and reasonably applied to avoid environmental risks

Knowledge, attitudes, and practices (KAP) regarding intra-abdominal pressure monitoring among pediatric intensive care nurses: A cross-sectional study

Objectives: This study aimed to assess the knowledge, attitudes, and practices (KAP) and the training requirements of pediatric intensive care nurses regarding intra-abdominal pressure (IAP) monitoring, in order to provide a reference for the development of relevant training programs and operational procedures in clinical practice. Methods: This descriptive cross-sectional survey was conducted from April 2023 to June 2023. A convenience sample was created by recruiting 212 pediatric intensive care nurses in eight hospitals in Zhejiang Province. A self-developed IAP monitoring KAP assessment tool was used for evaluation, which included knowledge (14 items), attitude (6 items), and practice (8 items), three dimensions, 28 items. Results: The overall KAP score was 60.73 ± 8.35; the knowledge score was 7.84 ± 2.35, with a scoring rate of 56.0%; the attitude score was 25.16 ± 3.23, with a scoring rate of 83.9%; and the practice score was 28.44 ± 6.46, with a scoring rate of 69.3%. Nurses wh-o have received IAP monitoring training have higher KAP score, knowledge score and practice score than those who have not received it (P  30 (P < 0.05). Among the participants, 55.7% of the nurses believed the current knowledge was insufficient to perform IAP measurement effectively. Difficulty in identifying the high-risk population of intra-abdominal hypertension (IAH) (64.6%), unfamiliarity with the operation process of IAP measurement (55.6%), and unreasonable nurse-patient ratio allocation (52.8%) were the main obstacles for nurses to monitor IAP. Conclusions: Pediatric intensive care nurses have a positive attitude towards IAP monitoring, but the knowledge level and practical behavior still need to be strengthened. In particular, the knowledge of published consensus definitions, measurement techniques, and frequency for IAP monitoring is inadequate. It is necessary to implement tailored IAP monitoring training based on their training needs and potential obstacles to promote the standardization and scientificity of IAP monitoring

Simultaneous Biofortification: Interaction between Zinc and Selenium Regarding Their Accumulation in Wheat

Wheat (Triticum aestivum L.) is a staple food worldwide, and agronomic biofortification with selenium (Se) and zinc (Zn) is a simple and effective way to increase nutrient intake. This study aimed to evaluate the combined effects of Zn and Se on the biofortification of wheat grain. Zinc sulfate (ZnSO4·H2O, 1.74, 2.61 and 3.48 kg Zn hm−2) and sodium selenite (Na2SeO3, 15 and 30 g Se hm−2) were sprayed individually or simultaneously at key stages of wheat growth (the jointing, booting, and filling stage) under field conditions. On the basis of soil Zn application, the foliar application of Zn or Se alone greatly increased grain Zn by 12.07–71.88% (up to 41.66–64.30 mg kg−1), and grain Se content by 131.81–527.21% (up to 0.21–0.50 mg kg−1), while the soil application of Zn had little effect on grain Zn. Compared with the foliar application of Zn or Se alone, the co-application of Se increased the grain Zn content by 1.74–16.15%, while the co-application of Zn significantly reduced grain Se content by 25.43–86.34% and the effect was more pronounced with an increase in Zn dosage. Moreover, positive correlations were found between Zn and copper (Cu) in grains, and wheat grains could provide adequate dietary intakes of manganese (Mn), Cu, and molybdenum (Mo) for humans. In summary, the soil application of 11 kg Zn hm−2 combined with the foliar application of 2.61 kg Zn hm−2 and 30 g Se·hm−2 is a feasible Zn-Se co-enrichment strategy, which would provide the recommended nutrient intake (RNI) of 113.63–124.72% (female) and 68.18–74.84% (male) of Zn and 81.30–95.85% of Se

Characterization of an Artificial Liver Support System-Related Vasovagal Reaction

Objective. An artificial liver support system (ALSS) is an effective therapy for patients with severe liver injury. A vasovagal reaction (VVR) is a common complication in various treatment settings but has not been reported previously in ALSS. Methods. This study retrospectively evaluated patients who suffered an ALSS-related VRR between January 2018 and June 2019. We collected data from VVR episodes including onset time, duration, changes in heart rate (HR) and blood pressure (BP), and drug treatment. Results. Among 637 patients who underwent ALSS treatment, 18 were included in the study. The incidence of VVR was approximately 2.82%. These patients were characterized by a rapid decrease in BP or HR with associated symptoms such as chest distress, nausea, and vomiting. The majority of patients (78%) suffered a VVR during their first ALSS treatment. Sixteen patients (89%) had associated symptoms after treatment began. Sixteen patients (89%) received human albumin or Ringer’s solution. Atropine was used in 11 patients (61%). The symptoms were relieved within 20 min in 15 patients and over 20 min in 3 patients. Conclusions. A VVR is a rare complication in patients with severe liver injury undergoing ALSS treatment. Low BP and HR are the main characteristics of a VVR