Precise Determination of the Crystallographic Orientations in Single ZnS Nanowires by Second-Harmonic Generation Microscopy

We report on the systematical study of the second-harmonic generation (SHG) in single zinc sulfide nanowires (ZnS NWs). The high-quality ZnS NWs with round cross-section were fabricated by chemical vapor deposition method. The transmission electron microscopy images show that the actual growth axis has a deviation angle of 0°∼20° with the preferential growth direction [120], which leads to the various polarization-dependent SHG response patterns in different individual ZnS NWs. The SHG response is quite sensitive to the orientations of <i>c</i> axis as well as the (100) and (010) crystal-axis of ZnS NWs; thus, all the three crystal-axis orientations of ZnS NWs are precisely determined by the SHG method. A high SHG conversion efficiency of 7 × 10^–6 is obtained in single ZnS NWs, which shows potential applications in nanoscale ultraviolet light source, nonlinear optical microscopy, and nanophotonic devices

Recreational use of horses in the New Forest Heritage Area Report to the New Forest Committee

SIGLEAvailable from British Library Document Supply Centre- DSC:q95/30029 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Additional file 2 of Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches

Additional file 2: Table S1

Additional file 6 of Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches

Additional file 6: Table S5

Additional file 7 of Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches

Additional file 7: Table S6

Additional file 5 of Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches

Additional file 5: Table S4

Additional file 4 of Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches

Additional file 4: Table S3

Additional file 3 of Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches

Additional file 3: Table S2

Additional file 1 of Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches

Additional file 1: FigureS1. Cells death determination under different concentrations of ethanoltreatment by methylene blue staining. A,0% ethanol treatment group; B, 3% ethanol treatment group; C, 6% ethanoltreatment group; D, 9% ethanol treatment group; E, 12% ethanol treatment group.Bar=100 μm.Figure S2.  Results of principalcomponent analysis (PCA) of the samples for transcriptome sequencing. FigureS3.  PCA score plots of the samples for metabolomicsanalysis in positive and negative ion modes. A, Positive ion mode; B, Negativeion mode. Table S7. Primersused in this study for real-time quantitative PCR detection

Synthetic Antibodies with a Human Framework That Protect Mice from Lethal Sudan Ebolavirus Challenge

The ebolaviruses cause severe and rapidly progressing hemorrhagic fever. There are five ebolavirus species; although much is known about Zaire ebolavirus (EBOV) and its neutralization by antibodies, little is known about Sudan ebolavirus (SUDV), which is emerging with increasing frequency. Here we describe monoclonal antibodies containing a human framework that potently inhibit infection by SUDV and protect mice from lethal challenge. The murine antibody 16F6, which binds the SUDV envelope glycoprotein (GP), served as the starting point for design. Sequence and structural alignment revealed similarities between 16F6 and YADS1, a synthetic antibody with a humanized scaffold. A focused phage library was constructed and screened to impart 16F6-like recognition properties onto the YADS1 scaffold. A panel of 17 antibodies were characterized and found to have a range of neutralization potentials against a pseudotype virus infection model. Neutralization correlated with GP binding as determined by ELISA. Two of these clones, E10 and F4, potently inhibited authentic SUDV and conferred protection and memory immunity in mice from lethal SUDV challenge. E10 and F4 were further shown to bind to the same epitope on GP as 16F6 with comparable affinities. These antibodies represent strong immunotherapeutic candidates for treatment of SUDV infection