Analysis of interspecies adherence of oral bacteria using a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis profiling.

Information on co-adherence of different oral bacterial species is important for understanding interspecies interactions within oral microbial community. Current knowledge on this topic is heavily based on pariwise coaggregation of known, cultivable species. In this study, we employed a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to systematically analyze the co-adherence profiles of oral bacterial species, and achieved a more profound knowledge beyond pairwise coaggregation. Two oral bacterial species were selected to serve as "bait": Fusobacterium nucleatum (F. nucleatum) whose ability to adhere to a multitude of oral bacterial species has been extensively studied for pairwise interactions and Streptococcus mutans (S. mutans) whose interacting partners are largely unknown. To enable screening of interacting partner species within bacterial mixtures, cells of the "bait" oral bacterium were immobilized on nitrocellulose membranes which were washed and blocked to prevent unspecific binding. The "prey" bacterial mixtures (including known species or natural saliva samples) were added, unbound cells were washed off after the incubation period and the remaining cells were eluted using 0.2 mol x L(-1) glycine. Genomic DNA was extracted, subjected to 16S rRNA PCR amplification and separation of the resulting PCR products by DGGE. Selected bands were recovered from the gel, sequenced and identified via Nucleotide BLAST searches against different databases. While few bacterial species bound to S. mutans, consistent with previous findings F. nucleatum adhered to a variety of bacterial species including uncultivable and uncharacterized ones. This new approach can more effectively analyze the co-adherence profiles of oral bacteria, and could facilitate the systematic study of interbacterial binding of oral microbial species

Robust interface between flying and topological qubits

Hybrid architectures, consisting of conventional and topological qubits, have recently attracted much attention due to their capability in consolidating the robustness of topological qubits and the universality of conventional qubits. However, these two kinds of qubits are normally constructed in significantly different energy scales, and thus this energy mismatch is a major obstacle for their coupling that supports the exchange of quantum information between them. Here, we propose a microwave photonic quantum bus for a direct strong coupling between the topological and conventional qubits, in which the energy mismatch is compensated by the external driving field via the fractional ac Josephson effect. In the framework of tight-binding simulation and perturbation theory, we show that the energy splitting of the topological qubits in a finite length nanowire is still robust against local perturbations, which is ensured not only by topology, but also by the particle-hole symmetry. Therefore, the present scheme realizes a robust interface between the flying and topological qubits. Finally, we demonstrate that this quantum bus can also be used to generate multipartitie entangled states with the topological qubits.Comment: Accepted for publication in Scientific Report