Tricin protects rats from ovariectomized-induced osteoporosis by enhancing Wnt/β-catenin pathway

Purpose: To investigate the effects of tricin on ovariectomized-induced osteoporosis, and unravel theunderling mechanism of action.Methods: An osteoporosis rat model was established by conducting ovariectomy (OVX). Changes in the microstructure of the trabecular bone were visualized using Hematoxylin and eosin (H&E) staining, and a three-point bending test was employed to assess the biomechanical stability of the femurs, after the administration of tricin (20 and 40 mg/kg). Subsequently, bone marrow mesenchymal stem cells (BMSCs) were isolated and treated with tricin (7 and 15 μM). Alizarin red staining was performed to assess mineralization, and Runt-related transcription factor 2 (RUNX2); osteocalcin (OCN) and collagen type I alpha 1 (Col1a1) were quantified using western blot analysis. The Wnt/β-catenin pathway related proteins, i.e., Wnt3a, β-catenin, glycogen synthase kinase-3 β (GSK-3 β) were determined.Results: Ovariectomy induced thinner and discontinuous trabecular bone, with increased marrow cavities, while application of tricin significantly improved the density and regularity meshwork, but reduced marrow cavities. Tricin also enhanced biomechanical competence as seen in the upregulated maximum load, stiffness, young modulus and maximum stress compared with OVX group (p < 0.01). Furthermore, tricin increased calcification in BMSCs, and significantly upregulated the expressions of RUNX2, OCN and COL1A1 when compared with OVX group (p < 0.01). It promoted Wnt/β-catenin signaling by enhancing Wnt3a and β-catenin, while inhibiting GSK3β expression, compared with OVX group (p < 0.05 or p < 0.01).Conclusion: Tricin exerts protective effects against OVX-induced osteoporosis by enhancing Wnt/β-catenin pathway. Thus, tricin is a potential therapeutic agent for the management of osteoporosis

Browning of Pig White Preadipocytes by Co-Overexpressing Pig PGC-1α and Mice UCP1

Background/Aims: Brown adipose tissue (BAT) is critical for mammals’ survival in the cold environment. BAT-dependent non-shivering thermogenesis is attributed to uncoupling protein 1 (UCP1)’s disengagement of oxidative phosphorylation from ATP synthesis and dissipates energy as heat. Thus individuals with a substantial amount of BAT are better equipped during cold stress and less likely to become obese. Recently, our laboratory has shown pig adipocytes have no UCP1 protein. The inability of newborn piglets to generate heat contributed to its high death rate. Repairing the genetic defect of UCP1 in pig adipocytes has implications in defending against cold for piglets and developing an alternative treatment for human obesity. Methods: Q-PCR, western blotting (WB) and oxygen consumption measurement were used to enable functional UCP1 protein in preadipocytes. Immunoprecipitation (IP), chromatin immunoprecipitation (CHIP), and dual-luciferase reporter assay system were used to clarify the thermogenesis mechanism of functional UCP1. Results: Only co-overexpressing mice UCP1 and pig PGC-1α increased not only the mitochondrial number but also the uncoupled respiration rate in the transfected pig adipocytes. The functional mice UCP1 increased the pig PGC-1α activity through the AMPK-SIRT1 pathway. The active form PGC-1α interacted with transcription factors Lhx8, Zic1, ERRα, and PPARα to regulate the expression of mitochondrial energy metabolism and adipocytes browning-related genes. Conclusion: Our data suggest a model in which pig PGC-1α and mice UCP1 work collaboratively to restore uncoupling respiration in pig preadipocytes. These results have great implications for piglet survival and developing an alternative treatment for human obesity in the future

An ex vivo human tumour assay reveals distinct patterns of EGFR trafficking in squamous cell carcinoma correlating to therapeutic outcomes

EGFR overexpression is associated with squamous cell carcinoma development. Altered endocytosis and polarization of receptor tyrosine kinases, including EGFR, affect migration and invasion in 3D culture. These studies have been completed via genetic sequencing, cell line or 3D in vitro and in vivo murine models. Here we describe an imaging method that allows ex-vivo examination of ligand-induced endocytosis of EGFR in non-dissociated human tumours. We analyzed sets of tumour samples from advanced cutaneous squamous cell carcinoma and Head and Neck squamous cell carcinoma, actinic keratosis, intra-epidermal carcinoma and cutaneous squamous cell carcinoma. We demonstrate that EGFR endocytosis is dysregulated in advanced SCC and correlates with anti-EGFR monoclonal antibody therapy outcomes. In actinic keratosis, intra-epidermal carcinoma and well-differentiated cutaneous squamous cell carcinoma different patterns of epidermal growth factor ligand uptake and binding were observed at the leading edge of different dysplastic lesions, suggesting that these differences in EGFR endocytosis might influence the metastatic potential of dysplastic squamous epithelium. These studies in live ex-vivo human tumours confirm that endocytosis dysregulation is a physiological event in human tumours and has therapeutic implications

KIAA0101 (KIAA0101)

Review on KIAA0101 (KIAA0101), with data on DNA, on the protein encoded, and where the gene is implicated

Hydrodynamic characteristic of synthetic jet steered underwater vehicle

In this paper, the hydrodynamic characteristic of a synthetic jet steered underwater vehicle is studied. The steering motion studied is the lateral motion and the yaw motion. The lateral motion is induced through the in-phase work of this two actuators and the yaw motion is realized through the out-of-phase work. The vehicle studied is REMUS AUV with synthetic jet actuator mounted inside. The hydrodynamic characteristic of the vehicle under different cruising speed is studied. The driving parameters of the SJ actuator keep invariant in different cases. When the two actuators work in phase, the average steering force is smaller than the thrust of the isolated actuator and keeps nearly invariant under different cruising speed. When the two actuators work out of phase, the average steering moment also keeps invariant with cruising speed. The mathematical model of the additional drag of the vehicle, the thrust of the actuator, the steering force as well as the steering moment is given. The velocity distribution is also given to assistant the analysis in this paper. From the analysis given it can be known the steering method based on SJ is realized through position control other than velocity control. (C) 2017 Elsevier Ltd. All rights reserved

Development and Performance Analysis of a New Self-Powered Magnetorheological Damper with Energy-Harvesting Capability

Magnetorheological (MR) dampers, used as intelligent semi-active vibration control devices to achieve low energy consumption, fast response, controllability, and other capabilities are generally installed with a variety of sensors on their exterior to ensure that the damping force can be accurately controlled. However, external sensors are often affected by external complications that reduce the reliability of the damper, and the cost of powering the damper coils in remote locations where power is not available can be significantly increased. Based on these problems, a new self-powered MR damper scheme is proposed. The proposed MR damper has both energy-harvesting capabilities and damping controllability, and greatly improves the stability and application range of the device by converting vibration energy into electrical energy to supply the excitation coil. The MR damper can drive the piston rod in a linear reciprocating motion by external excitation, which converts mechanical energy into electrical energy via a DC brushless three-phase generator after conversion by a double-linkage mechanism. At the same time, the electrical energy generated by the generator is passed into the excitation coil to change the output damping force of the damper. Meanwhile, the damping performance and energy-harvesting efficiency of the new self-powered MR damper is experimentally tested. Experimental results show the damping force of the device reaches 1040 N when the applied current is 0.6 A. The proposed self-powered MR damper has an instantaneous voltage amplitude of 1.782 V and a peak phase power of 4.428 W when the input excitation amplitude is 12.5 mm and the frequency is 3 Hz

Toward High Altitude Airship Ground-Based Boresight Calibration of Hyperspectral Pushbroom Imaging Sensors

The complexity of the single linear hyperspectral pushbroom imaging based on a high altitude airship (HAA) without a three-axis stabilized platform is much more than that based on the spaceborne and airborne. Due to the effects of air pressure, temperature and airflow, the large pitch and roll angles tend to appear frequently that create pushbroom images highly characterized with severe geometric distortions. Thus, the in-flight calibration procedure is not appropriate to apply to the single linear pushbroom sensors on HAA having no three-axis stabilized platform. In order to address this problem, a new ground-based boresight calibration method is proposed. Firstly, a coordinate’s transformation model is developed for direct georeferencing (DG) of the linear imaging sensor, and then the linear error equation is derived from it by using the Taylor expansion formula. Secondly, the boresight misalignments are worked out by using iterative least squares method with few ground control points (GCPs) and ground-based side-scanning experiments. The proposed method is demonstrated by three sets of experiments: (i) the stability and reliability of the method is verified through simulation-based experiments; (ii) the boresight calibration is performed using ground-based experiments; and (iii) the validation is done by applying on the orthorectification of the real hyperspectral pushbroom images from a HAA Earth observation payload system developed by our research team—“LanTianHao”. The test results show that the proposed boresight calibration approach significantly improves the quality of georeferencing by reducing the geometric distortions caused by boresight misalignments to the minimum level