The impact on the soil microbial community and enzyme activity of two earthworm species during the bioremediation of pentachlorophenol-contaminated soils

The ecological effect of earthworms on the fate of soil pentachlorophenol (PCP) differs with species. This study addressed the roles and mechanisms by which two earthworm species (epigeic Eisenia fetida and endogeic Amynthas robustus E. Perrier) affect the soil microbial community and enzyme activity during the bioremediation of PCP-contaminated soils. A. robustus removed more soil PCP than did E. foetida. A. robustus improved nitrogen utilisation efficiency and soil oxidation more than did E. foetida, whereas the latter promoted the organic matter cycle in the soil. Both earthworm species significantly increased the amount of cultivable bacteria and actinomyces in soils, enhancing the utilisation rate of the carbon source (i.e. carbohydrates, carboxyl acids, and amino acids) and improving the richness and evenness of the soil microbial community. Additionally, earthworm treatment optimized the soil microbial community and increased the amount of the PCP-4-monooxygenase gene. Phylogenic classification revealed stimulation of indigenous PCP bacterial degraders, as assigned to the families Flavobacteriaceae, Pseudomonadaceae and Sphingobacteriacea, by both earthworms. A. robustus and E. foetida specifically promoted Comamonadaceae and Moraxellaceae PCP degraders, respectively

Insight Into Metabolic Versatility of an Aromatic Compounds-Degrading Arthrobacter sp. YC-RL1

The genus Arthrobacter is ubiquitously distributed in different natural environments. Many xenobiotic-degrading Arthrobacter strains have been isolated and described; however, few have been systematically characterized with regard to multiple interrelated metabolic pathways and the genes that encode them. In this study, the biodegradability of seven aromatic compounds by Arthrobacter sp. YC-RL1 was investigated. Strain YC-RL1 could efficiently degrade p-xylene (PX), naphthalene, phenanthrene, biphenyl, p-nitrophenol (PNP), and bisphenol A (BPA) under both separated and mixed conditions. Based on the detected metabolic intermediates, metabolic pathways of naphthalene, biphenyl, PNP, and BPA were proposed, which indicated that strain YC-RL1 harbors systematic metabolic pathways toward aromatic compounds. Further, genomic analysis uncovered part of genes involved in the proposed pathways. Both intradiol and extradiol ring-cleavage dioxygenase genes were identified in the genome of strain YC-RL1. Meanwhile, gene clusters predicted to encode the degradation of biphenyl (bph), para-substituted phenols (npd) and protocatechuate (pca) were identified, and bphA1A2BCD was proposed to be a novel biphenyl-degrading gene cluster. The complete metabolic pathway of biphenyl was deduced via intermediates and functional gene analysis (bph and pca gene clusters). One of the these genes encoding ring-cleavage dioxygenase in bph gene cluster, a predicted 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC) gene, was cloned and its activity was confirmed by heterologous expression. This work systematically illuminated the metabolic versatility of aromatic compounds in strain YC-RL1 via the combination of metabolites identification, genomics analysis and laboratory experiments. These results suggested that strain YC-RL1 might be a promising candidate for the bioremediation of aromatic compounds pollution sites

Effects of two ecological earthworm species on atrazine degradation performance and bacterial community structure in red soil

Vermicomposting is an effective and environmentally friendly approach for eliminating soil organic contamination. Atrazine is one of the most commonly applied triazinic herbicides and frequently detected in agricultural soils. This study investigated the roles and mechanisms of two earthworm species (epigeic Eisenia foetida and endogeic Amynthas robustus) in microbial degradation of atrazine. Both earthworms accelerated atrazine degradation performance from 39.0% in sterile soils to 94.9%–95.7%, via neutralizing soil pH, consuming soil humus, altering bacterial community structure, enriching indigenous atrazine degraders and excreting the intestinal atrazine-degrading bacteria. Rhodoplanes and Kaistobacter were identified as soil indigenous degraders for atrazine mineralization and stimulated by both earthworm species. A. robustus excreted the intestinal Cupriavidus and Pseudomonas, whereas Flavobacterium was released by E. foetida. This study provides a comprehensive understanding of the distinct effects of two earthworm species on soil microbial community and atrazine degradation, offering technical supports to apply vermicomposting in effective soil bioremediation

Pore-Scale Imaging of the Oil Cluster Dynamic during Drainage and Imbibition Using In Situ X-Ray Microtomography

We imaged water-wet and oil-wet sandstones under two-phase flow conditions for different flooding states by means of X-ray computed microtomography (μCT) with a spatial resolution of 2.1 μm/pixel. We systematically study pore-scale trapping of the nonwetting phase as well as size and distribution of its connected clusters and disconnected globules. We found a lower Sor, 19.8%, for the oil-wet plug than for water-wet plug (25.2%). Approximate power-law distributions of the water and oil cluster sizes were observed in the pore space. Besides, the τ value of the wetting phase gradually decreased and the nonwetting phase gradually increased during the core-flood experiment. The remaining oil has been divided into five categories; we explored the pore fluid occupancies and studied size and distribution of the five types of trapped oil clusters during different drainage stage. The result shows that only the relative volume of the clustered oil is reduced, and the other four types of remaining oil all increased. Pore structure, wettability, and its connectivity have a significant effect on the trapped oil distribution. In the water sandstone, the trapped oil tends to occupy the center of the larger pores during the water imbibition process, leading to a stable specific surface area and a gradually decreasing oil capillary pressure. Meanwhile, in oil-wet sandstone, the trapped oil blobs that tend to occupy the pores corner and attach to the walls of the pores have a large specific surface area, and the change of the oil capillary pressure was not obvious. These results have revealed the well-known complexity of multiphase flow in rocks and preliminarily show the pore-level displacement physics of the process

Development Performance and Pressure Field Evolution of ASP Flooding

ASP (alkali-surfactant-polymer) is acknowledged as an effective technology to improve the oil recovery. The microscopic displacement efficiency and macroscopic sweep efficiency have been discussed in detail for the past few years. However, development performance, especially pressure characteristics, needs to be further studied. This paper aims to explore the pressure evolution performance during ASP flooding, of which the results will shed light on development characteristics of ASP flooding. The study on ASP flooding pressure field development is conducted by laboratory and numerical methodology. A large sandpack laboratory model with vertical heterogeneous layers is used to monitor pressure performance during the ASP flooding. With the help of interpolation methods, a precise and intuitive pressure field is obtained based on pressure data acquired by limited measurement points. Results show that the average formation pressure and its location are changing all the time in the whole process. In addition, the influence of heterogeneity and viscosity on recovery and pressure is also probed in this paper. We built a numerical simulation model to match the experiment data considering the physical and chemical alternation in ASP flooding. Also, response surface methodology (RSM) is adopted to obtain the formula between pressure functions and influencing factors

ERF transcription factors involved in salt response in tomato

ERF transcription factors are important regulatory components of ethylene signaling, known to be involved in plant development and stress responses by regulating the expression of ethylene responsive genes. However, relatively less is known about the function of ERF gene family in wild tomato response to salt stress. Previously, we have carried out a microarray analysis on the salt-responsive genes in salt tolerant wild tomato Solanum pimpinellifolium 'PI365967'. Five ERF genes (SpERFs) with induced expression upon salt treatment were subsequently selected for further characterization. Sequence analysis revealed that the seven amino acid residues in ERF proteins which are critical for their binding to the GCC-box in the promoter of ethylene responsive genes were conserved in five SpERFs. Two of five SpERFs (SpERF.B7 and SpERF.C11) belong to B3 sub-group as predicted by phylogenetic analysis. Expression analysis indicated that two SpERFs of B3 sub-group were more strongly induced in salt-tolerant genotypes than in salt-sensitive genotypes, in the order of PI365967 > LA2711 > Moneymaker, suggesting a positive role of B3 sub-group SpERFs in salt stress in tomato. To further confirm the function of B3 SpERFs in salt response, SpERF.B7 (one of two B3 SpERFs) was over-expressed in Arabidopsis. Consistent with our expectation, transgenic lines exhibited enhanced salt tolerance. These results will provide useful information for improving salt tolerance in tomato breeding in the future