Additional file 2: Figure S2. of Immune modulation of CD4+CD25+ regulatory T cells by zoledronic acid

The effect of ZA on Treg cells apoptosis. (A) Treg cells were treated with and without ZA (10, 50 and 100 μM) for 24 h. Apoptosis was measured by Annexin V‑FITC/PI staining and flow cytometry. (B) Quantitative analysis of Treg cells by flow cytometry revealed no effect of ZA on Treg cell apoptosis. (TIF 131 kb

Additional file 1: Figure S1. of Immune modulation of CD4+CD25+ regulatory T cells by zoledronic acid

The effect of ZA on CD4+ lymphocyte proliferation. (A) Isolated lymphocytes were labeled with CFSE, cultured in medium with or without 10 μM ZA and sensitized with anti-CD3 and anti-CD28 antibodies. (B) CD4+ lymphocyte proliferation curves were measured based on the percentage of cells with decreased fluorescence as compared to non-proliferating cells (0.4% at day 1). Data represent the mean values ± SEM and results from three independent experiments are shown. (TIF 134 kb