The distributions of demographical characteristics and clinical parameters in 708 controls and 354 patients with HCC.

<p>The distributions of demographical characteristics and clinical parameters in 708 controls and 354 patients with HCC.</p

Odds ratio (OR) and 95% confidence interval (CI) of clinical status and <i>WWOX</i> rs12918952 genotypic frequencies in 252 male HCC patients.

<p>Odds ratio (OR) and 95% confidence interval (CI) of clinical status and <i>WWOX</i> rs12918952 genotypic frequencies in 252 male HCC patients.</p

Association of <i>WWOX</i> genotypic frequencies with HCC laboratory status.

<p>Association of <i>WWOX</i> genotypic frequencies with HCC laboratory status.</p

Structural characterization and SNP (rs12918952) in human WWOX protein (NP_057457.1).

<p>Alignments of conserved domain-based sequences of (A) two conserved tryptophans domain (WW; cd00201) and (C) classical-like SDR domain (human_WWOX-like_SDR_c-like; cd09809) by use of multiple sequence alignment format and numbered according to the human WWOX is shown above the sequences. Strick consensus amino acids in the putative active centers of compact structural units are shown: the key amino acids of active site and cofactor binding site are highlighted in red star and blue pound signs, respectively. The WWOX-relative sequences are as follows: human (<i>H</i>. <i>sapiens</i>, NP_057457.1); mouse (<i>M</i>. <i>musculus</i>, NP_062519.2); chicken (<i>G</i>. <i>gallus</i>, NP_001025745.1); fish (<i>D</i>. <i>rerio</i>, NP_957207.1) and fly (<i>D</i>. <i>melanogaster</i>, NP_609171.1). (B) Schematic representation of the overall human WWOX protein; domain symbols are drawn approximately to scale. The rectangle represents the WW and human_WWOX-like_SDR_c-like domain; the key residues of active site and cofactor binding site are highlighted in red and triangle sign, respectively. The N-terminal and C-terminal ends are indicated (N’ and C’, respectively). (D) Ribbon diagram showing the homologous 3D model of the SDR_c-like domain of human WWOX using the SWISSMODEL server based on <i>M</i>. <i>abscessus</i> short chain dehydrogenase or reductase crystal structure (PDB ID: 3RIH). The side chains of the amino acids characterized catalytic tetrad of Asn232-Thr266-Tyr293-Lys297 are shown as sticks and labeled which aligned well with the ‘classical’ type of short-chain dehydrogenase/reductase (SDR) enzyme. The blue spheres represent the putative coenzyme NAD (P)-binding pocket is drawn to illustrate the location of the cofactor binding site. The ribbons indicate the backbone course of human WWOX and the arrows represent b-strands, and cylinders indicate a-helices. The figure was prepared using ViewerLite^™ 5.0 software. (E) Expression quantitative trait locus association between rs12918952 genotype and WWOX expression in whole blood (GTEx data set). Numbers in parentheses indicate the number of cases.</p

Odds ratio (OR) and 95% confidence interval (CI) of clinical status and <i>WWOX</i> rs11545028 genotypic frequencies in 354 HCC patients.

<p>Odds ratio (OR) and 95% confidence interval (CI) of clinical status and <i>WWOX</i> rs11545028 genotypic frequencies in 354 HCC patients.</p

Association of FGFR4 genotypic frequencies with the HCC laboratory findings.

<p>Association of FGFR4 genotypic frequencies with the HCC laboratory findings.</p

Liver cirrhosis status, virus status, and FGFR4 genotypic frequencies in 289 patients with HCC.

<p>Liver cirrhosis status, virus status, and FGFR4 genotypic frequencies in 289 patients with HCC.</p

The AFP level of the homozygous Arg/Arg genotype of FGFR4 rs351855 compared with that of the Gly/Gly genotype (p = 0.018).

<p>The AFP level of the homozygous Arg/Arg genotype of FGFR4 rs351855 compared with that of the Gly/Gly genotype (p = 0.018).</p

The distribution frequency of FGFR4 genotypes in 595 healthy controls and 289 patients with HCC.

<p>The odds ratios (ORs) with their 95% confidence intervals (CIs) were estimated using logistic regression models. The adjusted odds ratios (AORs) with their 95% Cis were estimated using multiple logistic regression models after controlling for age and sex. * p < 0.05 was considered statistically significant.</p><p>The distribution frequency of FGFR4 genotypes in 595 healthy controls and 289 patients with HCC.</p

Clinical TNM stage status and FGFR4 genotypic frequencies in 289 patients with HCC.

<p>> T2: multiple tumors larger than 5 cm or tumor involving a major branch of the portal or hepatic vein(s).</p><p>Clinical TNM stage status and FGFR4 genotypic frequencies in 289 patients with HCC.</p