How many metals does it take to fix N2? A mechanistic overview of biological nitrogen fixation

During the process of biological nitrogen fixation, the enzyme nitrogenase catalyzes the ATP-dependent reduction of dinitrogen to ammonia. Nitrogenase consists of two component metalloproteins, the iron (Fe) protein and the molybdenum-iron (MoFe) protein; the Fe protein mediates the coupling of ATP hydrolysis to interprotein electron transfer, whereas the active site of the MoFe protein contains the polynuclear FeMo cofactor, a species composed of seven iron atoms, one molybdenum atom, nine sulfur atoms, an interstitial light atom, and one homocitrate molecule. This Perspective provides an overview of biological nitrogen fixation and introduces three contributions to this special feature that address central aspects of the mechanism and assembly of nitrogenase

Nitrogenase: A nucleotide-dependent molecular switch

In the simplest terms, the biological nitrogen cycle is the reduction of atmospheric dinitrogen (N2) to ammonia with the subsequent reoxidation ammonia to dinitrogen (1). At the reduction level of ammonia, nitrogen incorporated into precursors for biological macromolecules such as proteins and nucleic acids. Reoxidation of ammonia to dinitrogen ("denitrification") by a variety of microbes (by way of nitrite and other oxidation levels of nitrogen) leads to the depletion of the "fixed," biologically usable, nitrogen pool. Besides the relatively small contribution from commercial ammonical fertilizer production, replenishing of the nitrogen pool falls mainly to a limited number of physiologically diverse microbes (e.g. eubacteria and archaebacteria; free-living and symbiotic; aerobic and anaerobic) that contain the nitrogenase enzyme system

Large diffractive/refractive apertures for space and airborne telescopes

Recent work, specifically the Lawrence Livermore National Laboratory (LLNL) Eyeglass and the DARPA MOIRE programs, have evaluated lightweight, easily packaged and deployed, diffractive/refractive membrane transmissive lenses as entrance apertures for large space and airborne telescopes. This presentation describes a new, innovative approach to the theory of diffractive and refractive effects in lenses used as telescope entrance apertures and the fabrication of the necessary large membrane optics. Analyses are presented to indicate how a broadband, highly transmissive diffractive / refractive membrane lens can be developed and fabricated, and potential applications in defense and astronomy are briefly discussed

Eisosomes Provide Membrane Reservoirs for Rapid Expansion of the Yeast Plasma Membrane

Cell surface area rapidly increases during mechanical and hypoosmotic stresses. Such expansion of the plasma membrane requires \u27membrane reservoirs\u27 that provide surface area and buffer membrane tension, but the sources of this membrane remain poorly understood. In principle, the flattening of invaginations and buds within the plasma membrane could provide this additional surface area, as recently shown for caveolae in animal cells. Here, we used microfluidics to study the rapid expansion of the yeast plasma membrane in protoplasts, which lack the rigid cell wall. To survive hypoosmotic stress, yeast cell protoplasts required eisosomes, protein-based structures that generate long invaginations at the plasma membrane. Both budding yeast and fission yeast protoplasts lacking eisosomes were unable to expand like wild-type protoplasts during hypoosmotic stress, and subsequently lysed. By performing quantitative fluorescence microscopy on single protoplasts, we also found that eisosomes disassembled as surface area increased. During this process, invaginations generated by eisosomes at the plasma membrane became flattened, as visualized by scanning electron microscopy. We propose that eisosomes serve as tension-dependent membrane reservoirs for expansion of yeast cells in an analogous manner to caveolae in animal cells

Iron mineralogy of a Hawaiian palagonitic soil with Mars-like spectral and magnetic properties

Visible and near-IR spectral data for some palagonitic soils from Mauna Kea, Hawaii, are similar to corresponding spectral data for Mars. It is important to understand the composition, distribution, and mineralogy of the ferric-bearing phases for the best spectral analogues because the correspondence in spectral properties implies that the nature of their ferric-bearing phases may be similar to those on Mars. In order to constrain interpretations of the Martian data, a variety of palagonitic soils should be studied in order to establish to what extent differences in their spectral data correspond to differences in the mineralogy of their ferric-bearing phases. Spectral (350-2100 nm), Mossbauer, magnetic, and some compositional data for one of a suite of Hawaiian palagonitic soils are presented. The soil (HWMK1) was collected below the biologically active zone from the sides of a gully cut at 9000 ft elevation on Mauna Kea. The soil was wet sieved with freon into seven size fractions less than 1 mm

Measurements of the ClO radical vibrational band intensity and the ClO + ClO + M reaction product

There is considerable interest in the kinetics and concentrations of free radicals in the stratosphere. Chlorine monoxide is a critically important radical because of its role in catalytic cycles for ozone depletion. Depletion occurs under a wide variety of conditions including the Antarctic spring when unusual mechanisms such as the BrO sub x/ClO sub x, ClO dimer (Cl sub 2 O sub 2), and ClO sub x/HO sub x cycles are suggested to operate. Infrared spectroscopy is one of the methods used to measure ClO in the stratosphere (Menzies 1979 and 1983; Mumma et al., 1983). To aid the quantification of such infrared measurements, researchers measured the ClO ground state fundamental band intensity

Profiting from Regulation: Evidence from the European Carbon Market

We investigate how cap-and-trade regulation affects profits. In late April 2006, the EU CO2 allowance price dropped 50 percent, equating to a € 28 billion reduction in the value of aggregate annual allowances. We examine daily returns for 552 stocks from the EUROSTOXX index. Despite reductions in environmental costs, we find that stock prices fell for firms in both carbon- and electricity-intensive industries, particularly for firms selling primarily within the EU. Our results imply that investors focus on product price impacts, rather than just compliance costs and the nominal value of pollution permits

Profiting from regulation: an event study of the EU carbon market

We investigate the effect of cap-and-trade regulation of CO2 on firm profits by performing an event study of a CO2 price crash in the EU market. We examine returns for 90 stocks from carbon intensive industries and 600 stocks in the broad EUROSTOXX index. Firms in carbon intensive, or electricity intensive industries, but not involved in international trade were most hurt by the event. This implies investors were focused on product price impacts, rather than compliance costs. We find evidence that firms\u27 net allowance positions also strongly influenced the share price response to the decline in allowance prices