Structural Reorganization of Parallel Actin Bundles by Crosslinking Proteins: Incommensurate States of Twist

We construct a coarse-grained model of parallel actin bundles crosslinked by compact, globular bundling proteins, such as fascin and espin, necessary components of filapodial and mechanosensory bundles. Consistent with structural observations of bundles, we find that the optimal geometry for crosslinking is overtwisted, requiring a coherent structural change of the helical geometry of the filaments. We study the linker-dependent thermodynamic transition of bundled actin filaments from their native state to the overtwisted state and map out the "twist-state'' phase diagram in terms of the availability as well as the flexibility of crosslinker proteins. We predict that the transition from the uncrosslinked to fully-crosslinked state is highly sensitive to linker flexibility: flexible crosslinking smoothly distorts the twist-state of bundled filaments, while rigidly crosslinked bundles undergo a phase transition, rapidly overtwisting filaments over a narrow range of free crosslinker concentrations. Additionally, we predict a rich spectrum of intermediate structures, composed of alternating domains of sparsely-bound (untwisted) and strongly-bound (overtwisted) filaments. This model reveals that subtle differences in crosslinking agents themselves modify not only the detailed structure of parallel actin bundles, but also the thermodynamic pathway by which they form.Comment: Main Text (25 pages, 7 figures) with supporting material (12 pages, 9 figures, 2 tables

NASA Limited Inflight Lab Sensor

The National Aeronautics and Space Administration (NASA) is currently developing the capabilities needed to send humans to Mars in 2030 for a three year mission. Astronaut health must be monitored monthly during such a lengthy mission. The UA Enterprise Senior Design Team worked to design a reusable, novel blood panel cartridge system to monitor astronaut health and to minimize waste production. Most current cartridges are composed of polydimethylsiloxane (PDMS). PDMS microchannels were fabricated to conduct simple proof of concept experiments for the design of a novel cartridge. To determine an ideal blood cleaning solution, bovine blood will be injected into the microchannels at a fixed flowrate and then cleaned using different reagents. The presence of blood and protein residue will then be assessed using trypan blue staining and fluorescently-tagged bovine serum albumin. In addition, a novel, reusable cartridge schematic was produced and modeled in AutoCAD and MATLAB. This design incorporated an additional inlet to allow for cleaning of the device and a novel streptavidin/biotin enzyme reservoir for reintroduction of fresh enzymes. Miniaturized analytical techniques as well as performance tests for each priority analytical method were incorporated in the final design. The proposed device shows promise for this NASA mission

Retired A Stars and Their Companions IV. Seven Jovian Exoplanets from Keck Observatory

We report precise Doppler measurements of seven subgiants from Keck Observatory. All seven stars show variability in their radial velocities consistent with planet-mass companions in Keplerian orbits. The host stars have masses ranging from 1.1 < Mstar/Msun < 1.9, radii 3.4 < Rstar/Rsun < 6.1, and metallicities -0.21 < [Fe/H] < +0.26. The planets are all more massive than Jupiter (Msini > 1 Mjup) and have semimajor axes > 1 AU. We present millimagnitude photometry from the T3 0.4m APT at Fairborn observatory for five of the targets. Our monitoring shows these stars to be photometrically stable, further strengthening the interpretation of the observed radial velocity variability. The orbital characteristics of the planets thus far discovered around former A-type stars are very different from the properties of planets around dwarf stars of spectral type F, G and K, and suggests that the formation and migration of planets is a sensitive function of stellar mass. Three of the planetary systems show evidence of long-term, linear trends indicative of additional distant companions. These trends, together with the high planet masses and increased occurrence rate, indicate that A-type stars are very promising targets for direct imaging surveys.Comment: PASP Accepted, final submission awaiting comments from the communit

The California Planet Survey II. A Saturn-Mass Planet Orbiting the M Dwarf Gl649

We report precise Doppler measurements of the nearby (d = 10.34 pc) M dwarf Gl649 that reveal the presence of a planet with a minimum mass Msini = 0.328 Mjup in an eccentric (e = 0.30), 598.3 day orbit. Our photometric monitoring reveals Gl649 to be a new variable star with brightness changes on both rotational and decadal timescales. However, neither of these timescales are consistent with the 600-day Doppler signal and so provide strong support for planetary reflex motion as the best interpretation of the observed radial velocity variations. Gl649b is only the seventh Doppler-detected giant planet around an M dwarf. The properties of the planet and host-star therefore contribute significant information to our knowledge of planet formation around low-mass stars. We revise and refine the occurrence rate of giant planets around M dwarfs based on the California Planet Survey sample of low-mass stars (M* < 0.6 Msun). We find that f = 3.4^{+2.2}_{-0.9}% of stars with M* < 0.6 Msun harbor planets with Msini > 0.3$ Mjup and a < 2.5 AU. When we restrict our analysis to metal-rich stars with [Fe/H] > +0.2 we find the occurrence rate is 10.7^{+5.9}_{-4.2}%.Comment: 8 pages, 4 figures, 3 tables, PASP accepte

Three Super-Earths Orbiting HD 7924

We report the discovery of two super-Earth mass planets orbiting the nearby K0.5 dwarf HD 7924 which was previously known to host one small planet. The new companions have masses of 7.9 and 6.4 M$_\oplus$, and orbital periods of 15.3 and 24.5 days. We perform a joint analysis of high-precision radial velocity data from Keck/HIRES and the new Automated Planet Finder Telescope (APF) to robustly detect three total planets in the system. We refine the ephemeris of the previously known planet using five years of new Keck data and high-cadence observations over the last 1.3 years with the APF. With this new ephemeris, we show that a previous transit search for the inner-most planet would have covered 70% of the predicted ingress or egress times. Photometric data collected over the last eight years using the Automated Photometric Telescope shows no evidence for transits of any of the planets, which would be detectable if the planets transit and their compositions are hydrogen-dominated. We detect a long-period signal that we interpret as the stellar magnetic activity cycle since it is strongly correlated with the Ca II H and K activity index. We also detect two additional short-period signals that we attribute to rotationally-modulated starspots and a one month alias. The high-cadence APF data help to distinguish between the true orbital periods and aliases caused by the window function of the Keck data. The planets orbiting HD 7924 are a local example of the compact, multi-planet systems that the Kepler Mission found in great abundance.Comment: Accepted to ApJ on 4/7/201

Time-stepping approach for solving upper-bound problems: Application to two-dimensional Rayleigh-Benard convection

An alternative computational procedure for numerically solving a class of variational problems arising from rigorous upper-bound analysis of forced-dissipative infinite-dimensional nonlinear dynamical systems, including the Navier-Stokes and Oberbeck-Boussinesq equations, is analyzed and applied to Rayleigh-Benard convection. A proof that the only steady state to which this numerical algorithm can converge is the required global optimal of the relevant variational problem is given for three canonical flow configurations. In contrast with most other numerical schemes for computing the optimal bounds on transported quantities (e.g., heat or momentum) within the "background field" variational framework, which employ variants of Newton's method and hence require very accurate initial iterates, the new computational method is easy to implement and, crucially, does not require numerical continuation. The algorithm is used to determine the optimal background-method bound on the heat transport enhancement factor, i.e., the Nusselt number (Nu), as a function of the Rayleigh number (Ra), Prandtl number (Pr), and domain aspect ratio L in two-dimensional Rayleigh-Benard convection between stress-free isothermal boundaries (Rayleigh's original 1916 model of convection). The result of the computation is significant because analyses, laboratory experiments, and numerical simulations have suggested a range of exponents alpha and beta in the presumed Nu similar to (PrRa beta)-Ra-alpha scaling relation. The computations clearly show that for Ra <= 10(10) at fixed L = 2 root 2, Nu <= 0.106Pr(0)Ra(5/12), which indicates that molecular transport cannot generally be neglected in the "ultimate" high-Ra regime.NSF DMS-0928098 DMS-1515161 DMS-0927587 PHY-1205219Simons FoundationNSFONRInstitute for Computational Engineering and Sciences (ICES

Radiological Features of Giant Cell Tumours of Bone

Introduction The aim of this study was to evaluate radiological measurements to establish the origin of giant cell tumours of bone. Methods A multi-centre retrospective review was conducted of patients with histologically confirmed giant cell tumours of bone. Images were analysed to estimate the centre of the tumour. Measured from the joint line, the ratio between the distance of the centre of the tumour and the physeal scar was calculated. Results Ninety-five patients were included in the study. Two observers found the tumour to be arising from the metaphyseal area in 94% - 97% of the cases. There was good agreement between the measurements of observers (interclass correlation coefficient 0.71). Conclusion Giant cell tumours of bone appear to be arising from the metaphyseal region

Comorbidities and Pregnancy Do Not Affect Local Recurrence in Patients With Giant Cell Tumour of Bone

This study evaluates the relationship between pregnancy, comorbid conditions and giant cell tumour of bone. Furthermore, it examines if pregnancy and comorbid conditions affect the outcome following treatment for this tumour. A multi-centre retrospective review was conducted of consecutive patients with a confirmed histological diagnosis of giant cell tumour of bone between June 2012 and May 2017. A total of 195 patients were identified from two centres. Of these, 168 patients were treated with curative intent and had more than six months follow-up. Data were collected on pregnancy status, comorbid conditions, site of disease, surgical management and local recurrence rates. Statistical analysis included the Fisher exact test and Kaplan-Meier survival analysis. There were 72 females of childbearing age, of which 15 (21%) were currently pregnant or had been pregnant within the last six months. The pregnancy rate is higher than the highest reported pregnancy rate over the last 10 years (8.4%; Fisher test, p = 0.033). Women were more likely to have a comorbid condition than men (Fisher test, p < 0.002) and had a higher rate of autoimmune disease than the normal population (p = 0.015). Men were older than women (Wilcoxon test, p = 0.046) and had less risk of local recurrence (logrank test, p = 0.014). Pregnancy or comorbid conditions did not increase the local recurrence rate. Predictors for local recurrence included location in the distal radius (logrank test, p < 0.001), intralesional treatment (logrank test, p = 0.008) and age less than 40 (logrank test, p = 0.043). In conclusion, giant cell tumour of bone is more common in pregnant females and patients with immune disease. Comorbidities and pregnancy do not affect the local recurrence rate. Male patients over 40 years of age have a lower risk of local recurrence, and patients with disease in the distal radius have a high risk of recurrence