<i>P</i>. <i>freudenreichii</i> subsp. <i>shermani</i> CIRM BIA 1 tolerance to digestive stresses.

<p>Propionibacteria were harvested at different stages of growth in CdM black square or in EJ white square, prior to acid (A, pH 2) or bile salts (B, 1 g L^-1) challenges. Surviving bacteria were then counted by CFU enumeration. Means with different lower case superscript letters (a-b) differ significantly (<i>P</i> < 0.05) in italic groups for CdM and in normal case groups for EJ.</p

Two-dimensional analysis of protein expression during growth of <i>P</i>. <i>freudenreichii</i> subsp. <i>shermani</i> CIRM BIA 1 in EJ and in CdM.

<p>(A) Neosynthesized proteins were radiolabeled with ³⁵S amino acids in both media during indicated duration: lag phase (early), the beginning of the exponential phase (early exponential), the exponential and early stationary phases. (B) Cellular proteins accumulated during growth were Coomassie blue stained.</p

Relative change in free amino acid concentration during growth of <i>P</i>. <i>freudenreichii</i> subsp. <i>shermani</i> CIRM BIA 1 in CdM (A, C and E) and EJ (B, D and F).

<p>(A and B) Alliphatic amino acids; (C and D) basic, acidic + amide amino acids; (E and F) Aromatic, hydroxyl and sulfur-containing amino acids. The arrows indicate the main growth phases corresponding to each incubation medium: exponential (Exp), early stationary (E.Stat) and late stationary phase (L.Stat).</p

Growth and survival of <i>P</i>. <i>freudenreichii</i> subsp. <i>shermani</i> CIRM BIA 1 in EJ and CdM

<p>(A) growth curve and (B) Live/dead staining of the bacterial cells in the exponential (Exp), early stationary (E.Stat) and late stationary phase (L.Stat) of growth in CdM (<b>black dot</b>) and in EJ (<b>white dot</b>).</p

Proteins differentially expressed after growth of <i>P</i>. <i>freudenreichii</i> subsp. <i>shermani</i> CIRM BIA 1 in EJ and in CdM and identified by on line coupling NanoLC-ESI-Q-TOF tandem mass spectrometry.

<p>*: Fold changes correspond to the protein overexpressed with a minimum fold change of 1.2 in the CdM or EJ determined by image analysis</p><p>**: e-value is the number of times a given peptide score will be achieved by incorrect matches from a database search. Protein identifications were automatically validated when they showed at least two unique peptides with an e-value below 0.05 corresponding to log(e-value) < -1.30</p><p>Proteins differentially expressed after growth of <i>P</i>. <i>freudenreichii</i> subsp. <i>shermani</i> CIRM BIA 1 in EJ and in CdM and identified by on line coupling NanoLC-ESI-Q-TOF tandem mass spectrometry.</p