5 research outputs found
Preparation and evaluation of a thermosensitive liposomal hydrogel for sustained delivery of danofloxacin using mesoporous silica nanoparticles
BACKGROUND: Sustained release delivery system can reduce
the dosage frequency and maintain the therapeutic level
of drugs for a longer time. Biodegradable, biocompatible
and thermosensitive chitosan-beta-glycerophosphate (C-GP)
solutions can solidify at body temperature and maintain their
physical integrity for a longer duration. OBJECTIVES: To develop
a novel delivery system based on the integration of
liposomes in hydrogel using mesoporous silica nanoparticles
(MSNs) for sustained release of danofloxacin in farm
animals. METHODS: The MSNs were prepared using N-cetyltrimethylammonium
bromide and tetraethylortho silica.
The liposomes were prepared by thin film hydration method.
C-GP solution containing danofloxacin-loaded MSN liposomes
underwent different in-vitro tests, including evaluation
of the entrapment efficiency, gelation time, morphology,
drug release pattern as well as antimicrobial activities against
S. aureus and E. coli. RESULTS: The mean pore size of MSNs
was 2.8 nm and the mean MSN entrapment efficiency was
45%. Kinetics of danofloxacin release from liposomal hydrogel
followed the Higuchi’s model. This formulation was
capable of sustaining the danofloxacin release for more than
96 h. The FTIR studies showed that there were no interactions
between danofloxacin and hydrogel excipients. Scanning
electron microscopy (SEM) showed that the formed gel
had a continuous texture, while the swelled gel in the phosphate
buffer had a porous structure. Microbiological tests
revealed a high antibacterial activity for lipomosal hydrogel
of danofloxacin-loaded MSN comparable with danofloxacin
solution. CONCLUSIONS: The liposomal hydrogel solidified at
body temperature, effectively sustained the release of danofloxacin
and showed in vitro antibacterial effects
Preparation and in vitro evaluation of chitosan-based films for the sustained delivery of enrofloxacin
The implantable drug products are developed mainly to sustain the drug release. This study was conducted to formulate and evaluate cross-linked films of chitosan/β-glycerophosphate (β-GP) for the sustained delivery of enrofloxacin (ENR). Two types of formulations, single-layer (F1 and F2) and triple-layer (F3 and
F4) films, were prepared. In vitro drug release, kinetic modelling, Fourier transform infrared spectroscopy (FTIR) spectra, morphological and microbiological studies were performed. Drug release from F1 and F2 continued up to 5 hours but from F3 and F4, it was extended over 96 and 168 hours, respectively. The
cumulative drug release for F1, F2, F3 and F4 were 72.6, 70.1, 90.5 and 82.4%, respectively. The inhibition zones of bacterial growth by using positive controls and single layer films were significantly greater than those of triple-layer films (p < 0.05), indicating sustained drug release pattern of the multi-layer films.These findings suggest that the triple-layer chitosan/ β-GP films could be effective to deliver ENR for a long period
Effect of 3,4-Methylenedioxymethamphetamine on Liver CYP2C19 Enzyme Activity in Isolated Perfused Rat Liver Using Omeprazole Probe
Background and purpose: This study aimed at investigating the effects of 3,4-Methyl​enedioxy​methamphetamine (MDMA) on liver cytochrome 2C19 enzyme activity, which is a major liver enzyme in the metabolism of a wide range of drugs, using omeprazole as a probe of the CYP2C19 activity in isolated perfused rat liver.
Materials and methods: This experimental study was done in 20 male Sprague–Dawley (SD) rats (weighing 250–300 g). After isolating the animal liver, omeprazole was administered at 400 μm and the concentration of omeprazole and its metabolite were determined. The liver was then washed with perfusion buffer, and MDMA was transferred at 300 ng/ml unilaterally from the same liver for 30 minutes. After re-washing the liver with perfusion buffer, omeprazole was passed through the liver for second time and the metabolic ratio was determined after exposure to MDMA. This process was also done in a group of animals at 600 ng/ml of MDMA.
Results: Analysis of data from three end-time intervals after exposure to liver at 300 and 600 ng/ml of MDMA, showed 26.6% and 20.6% reduction in the activity of CYP2C19. Findings showed that MDMA administration could significantly reduce the activity of CYP2C19.
Conclusion: According to this study, liver exposure to MDMA can significantly reduce cytochrome 2C19 activity, but, further studies are needed to examine this issue more closely