Electromyographic Evaluation of Superior Orbicularis Oris Muscle Activity in Patients With Complete Cleft Lip and Cleft Palate Before and After Vestibuloplasty Using Carbon Dioxide Laser Therapy: Co2 laser therapy on Electromyography after vestibuloplasty in cleft lip patients

Introduction: Cleft lip and cleft palate are the most typical congenital craniofacial anomalies. Postsurgical scars in cleft lip patients can cause shallow upper labial sulcus and reduced upper lip length. The aim of the present study was to evaluate the impact of CO2 laser vestibuloplasty on the electromyographical (EMG) activity of patients’ superior orbicularis oris muscle, depth of labial sulcus, and upper lip length, and their satisfaction with the procedure and its results. The CO2 laser is an effective vestibuloplasty procedure, is safe and highly acceptable for patients, and has little effect on muscle contraction.Methods: A total of 15 cleft lip and cleft palate patients participated in this study. Vestibuloplasty was performed using carbon dioxide laser therapy in one session in such a way that EMG activity in the upper lip muscle, depth of labial sulcus, and upper lip length were measured two times: before (T0) and twenty days after surgery (T1). Patients’ satisfaction surveys were also recorded using a patient questionnaire at T1.Results: The mean values for vestibular depth at T0 and T1 were 2.00±1.71 and 4.52±1.43, respectively. The mean values for upper lip length at T0 and T1 were 6.90±2.32 and 8.00±2.40, respectively, suggesting a significant change in vestibular depth and upper lip length. The vestibular depth and upper lip length significantly increased at T1. The EMG recording of the upper lip muscle experienced a moderate decrease after laser treatment, both at rest and at maximum lip protrusion. Moreover, patient’s satisfaction with the surgical procedure was 82% positive.Conclusion: Using the CO2 laser increased the vestibular depth and upper lip length successfully and made small changes in the EMG activity of the superior orbicularis oris

Diode Laser Irradiation Effects on Miniscrew Stability and IL-1β and TGF-β1 Levels: A Split-Mouth Randomized Controlled Clinical Trial: Diode Laser Effects on Miniscrew Stability

Introduction: This study aimed to assess the effect of low-level laser therapy (LLLT) on miniscrew stability and concentrations of interleukin-1β (IL-1β) and transforming growth factor-beta (TGF-β1) in peri-miniscrew crevicular fluid in the course of orthodontic treatment. Methods: This randomized split-mouth double-blind clinical trial evaluated 18 patients requiring anterior retraction along with maximum anchorage. Miniscrews were placed between the maxillary second premolar and first molar. A diode laser was irradiated with a 980-nm wavelength and 100-mW output power in continuous-wave mode at four-time points: T0 (1 hour after miniscrew placement), T1 (1 week later), T2 (at 1 month) and T3 (at 3 months) in one quadrant of the maxilla (laser group). The other quadrant of the maxilla underwent the pseudo-application of the laser (control group). The primary stability of mini-screws was measured by Periotest M and reported as Periotest value (PTV). Also, at each time point, samples were collected from the peri-miniscrew crevicular fluid one hour after laser irradiation to assess the concentration of IL-1β and TGF-β1. Results: The mean PTV (inverse of the stability) was smaller in the laser group compared with the control group at all time points; this difference was significant at T2 and T3. The mean concentration of IL-1β in the laser group was lower than that in the control group at all time points, and this difference was significantly remarkable at T0 and T3. The mean concentration of TGF-β1 in the laser group was lower than that in the control group at T0, T1, and T3; however, the difference was not statistically significant. Conclusions: The current results supported the efficacy of LLLT in increasing the miniscrew stability and decreasing the level of IL-1β pro-inflammatory cytokin

The effect of ovalbumin on orthodontic induced root resorption

Background: This randomized trial was undertaken to investigate the effect of experimentally induced allergy on orthodontic induced root resorption. Materials and Methods: A total of 30 Wistar rats were divided randomly into test and control groups. Starting from the first 3 days, the rats in the test group were injected intra-peritoneally by 2 mg ovalbumin as allergen and 0.5 mg Alume as adjuvant. Afterward only allergen was injected once a week. The control group was injected by normal saline. After 21 days, Wistar immunoglobulin E was measured and peripheral matured eosinophil was counted. A total of 50 g nickel-titanium closed coil spring was ligated between right incisor and first molar. All animals were sacrificed after 14 days. The mesial root of the right and left first molar was dissected in a horizontal plane. The specimens were divided into four groups considering whether force and/or ovalbumin was applied or not. Root resorption was measured and compared among these groups. Repeated measures analysis of variance (ANOVA), and Bonferoni tests were used to analyze the data. The level of significance was determined at 0.05. Results: In general, the differences were insignificant (P > 0.05). As the only exception, the group in which both ovalbumin and force were applied had significantly more root resorption than the group in which neither force nor ovalbumin was applied (P < 0.001). Conclusion: Allergy may increase the susceptibility to root resorption. Application of light force, periodical monitoring of root resorption and control of allergy are advisable