HID-1 controls formation of large dense core vesicles by influencing cargo sorting and trans-Golgi network acidification

Large dense core vesicles (LDCVs) mediate the regulated release of neuropeptides and peptide hormones. They form at the trans-Golgi network (TGN), where their soluble content aggregates to form a dense core, but the mechanisms controlling biogenesis are still not completely understood. Recent studies have implicated the peripheral membrane protein HID-1 in neuropeptide sorting and insulin secretion. Using CRISPR/Cas9, we generated HID-1 KO rat neuroendocrine cells, and we show that the absence of HID-1 results in specific defects in peptide hormone and monoamine storage and regulated secretion. Loss of HID-1 causes a reduction in the number of LDCVs and affects their morphology and biochemical properties, due to impaired cargo sorting and dense core formation. HID-1 KO cells also exhibit defects in TGN acidification together with mislocalization of the Golgi-enriched vacuolar H+-ATPase subunit isoform a2. We propose that HID-1 influences early steps in LDCV formation by controlling dense core formation at the TGN.</jats:p

Guaranteeing Canadian lamb meat quality using near infrared spectroscopy on intact rack

Lamb racks from commercial carcasses were scanned using near infrared spectroscopy (NIRS). The predictions accuracies (R2) for meat quality traits were assessed. Prediction accuracy ranged between 0.40 and 0.94. When predicted values were used to classify meat based on quality, 88.7- 95.2% of samples were correctly classified as quality-guaranteed.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Exploration of methods for lamb carcass yield estimation in Canada

Different approaches were evaluated to improve the accuracy of carcass yield predictions of Canadian lamb carcasses using manually obtained measurements and dual-energy X-ray absorptiometry (DEXA). Several linear carcass measurements were obtained from a population of commercial lamb carcasses representative of the variability in Canadian slaughter plants (n = 155). Carcass measures were categorized into four sets according to when each measure could be obtained in the slaughter process. Each set of carcass measurements were subjected to stepwise regression and used to develop models for the estimation of lean meat and saleable yield percentages. Tissue depth measures (at the GR site) explained 44% of variation in lean meat yield in hot carcasses and 53% in cold carcasses. When additional parameters were included with cold GR, the regression model explained 61.9% of the variability in lean meat yield. Saleable yield predictions were less accurate (R2 < 0.40); the greatest degree of variability was predicted when the model included ribeye area (R2 = 0.39). The DEXA scans obtained on carcass sides were able to predict about 78% of variability in carcass lean meat yield and 91% of fat content. This information could be used by the lamb meat industry to establish new carcass classification systems based on more accurate lean meat yield values.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author