Effects of PU, USG and NPK briquette on nitrogen use efficiency and yield of BR22 rice under reduced water condition

An experiment was conducted at the Soil Science Field Laboratory of Bangladesh Agricultural University, Mymensingh during the aman season of 2012 to investigate the effects of prilled urea (PU), urea super granule (USG) and NPK briquette on NH4- N concentration in field water, yield and nitrogen (N) use efficiency (NUE) of BR22 rice under reduced water conditions. The experiment was laid out in a randomized complete block design with three replications. There were altogether eight treatment combinations viz. T1: Control (No N fertilizer), T2: 52 kg N ha-1 from USG, T3: 104 kg N ha-1 from USG, T4: 78 kg N ha-1 from PU, T5: 120 kg N ha-1 from PU, T6: 51 kg N ha-1 from NPK briquette, T7: 78 kg N ha-1 from USG and T8: 78 kg N ha-1 from NPK briquette. Water samples were collected from rice field for seven consecutive days after deep placement of USG and the first split application of PU and the samples were analyzed for NH4-N. The Highest concentration of NH4-N in water was observed at the second day of PU application followed by gradual decrease with time. The yield contributing characters like plant height, panicle length, number of effective tillers hill-1 and grains panicle-1 were significantly influenced by different treatments. The highest grain yield of 3.93 t ha-1 was recorded from 104 kg N ha-1 as USG (T3) and the lowest value of 2.12 t ha-1 was obtained from control. The N use efficiency was increased when the N was applied as USG. The overall results revealed that application of USG and NPK briquette may be practised for obtaining better yields in addition to increasing the efficiency of N fertilizer

Strength development in fine-grained paddy field soil by lime addition

Due to high population density, the people of Bangladesh are building houses and the government is establishing infrastructure (such as roads, flyovers, bridges) on agricultural land that pose significant settlement issues. Consequently, such development works require prior improvement of the soft soil or improvement during construction to restrict post-construction failures. Soil stabilization, a conventionally used ground improvement method, is often utilized to alter engineering properties of fine-grained soil such as strength, stability, permeability, weathering resistance, etc. to maintain the required construction criteria. Moreover, the addition of lime in the soil is demonstrated to markedly reduce the hydraulic conductivity, which will prevent the vertical movement of pore water. With this in mind, in the current study an attempt has been undertaken to increase the strength of fine-grained soil of agricultural land by adding commercially available hydrated lime, which may in the future be used as foundation material. For this purpose, lime was added at various percentages (0–12%) by weight to soil collected from a paddy field. The study noted the collected soils as being inorganic silts of high plasticity or organic clays of medium to high plasticity. Unconfined compression tests were conducted on cylindrical mold prepared specimens with clayey soil in the presence of lime, and all specimens were cured for 3–90 days for strength development. The study revealed 7% added lime as the optimum content, with the corresponding unconfined compressive strength (UCS) observed to be 344 kPa and 356 kPa at the end of 28 days and 90 days, respectively. The evolution of unconfined compressive strength was higher in hotter and more alkaline environments

TEMPORAL VARIATION IN SEED QUALITY OF INDIAN SPINACH PRESERVED IN DIFFERENT CONTAINERS

The experiment was conducted to investigate the seed quality of Indian spinach for three months stored at different containers (Plastic pot, polythene bag and cloth bag) during 2013-2014 at Seed Technology Laboratory of Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur. The initial moisture content of the seed was 9.89% with 75.00% germination. However, after two months, the seed had a different range of increased moisture content with reduced germination percentage depending upon the packing materials. Among the three containers, seeds stored at cloth bag absorbed more moisture (14.36%) from surrounding atmosphere followed by the moisture content, 12.67% and 11.50% of polythene bag and airtight plastic pot, respectively. Seeds stored at plastic pot, polythene bag and cloth bag exhibited 65.00, 64.00 and 50.00 per cent germination, respectively. Similarly, the fresh weight and dry weight of seedling and seedling vigor were also decreased. The seeds of plastic pot were good in term of germination capacity, fresh and dry weight and vigor index in comparison to those stored at cloth bag and polythene bag

Quantitative Detection of Promoter Hypermethylation as a Biomarker of Acute Kidney Injury During Transplantation

Aberrant promoter hypermethylation, also known as epigenetics, is thought to be a promising biomarker approach to diagnose malignancies. Kidney repair after injury is a recapitulation of normal morphogenesis, with similarities to malignant transformation. We hypothesized that changes in urine epigenetics could be a biomarker approach during early kidney transplant injury and repair. We examined urine DNA for aberrant methylation of 2 gene promoters (DAPK CALCA) by quantitative methylation specific PCR from 13 deceased and 10 living donor kidney transplant recipients on postoperative day 2 and 65 healthy controls. Results were compared with clinical outcomes and to results of the kidney biopsy. Transplant recipients were significantly more likely to have aberrant hypermethylation of the CALCA gene promoter in urine than healthy controls (100% vs. 31%, p<0.0001). There was increased CALCA hypermethylation in the urine of deceased vs. living donor transplants (21.60 +/− 12.5 vs. 12.19 +/− 4.7 P=0.04). Furthermore, there was a trend towards increased aberrant hypermethylation of urine CALCA in patients with biopsy-proven acute tubular necrosis vs. acute rejection and slow or prompt graft function (mean: 20.40 +/− 6.9, 13.87 +/− 6.49, 17.17 +/− 13.4, P=0.67). However, there was no difference of CALCA hypermethylation in urine of patients with delayed graft function vs. those with slow or prompt graft function (16.9 +/− 6.2 vs. 18.5 +/− 13.7, respectively; P=0.5). There was no aberrant hypermethylation of DAPK in the urine of transplant patients. Urine epigenetics is a promising biomarker approach for acute ischemic injury in transplantation that merits future study

Virology Journal

Background: Genomic epidemiology has helped reconstruct the global and regional movement of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, there is still a lack of understanding of SARS-CoV-2 spread in some of the world’s least developed countries (LDCs). Methods: To begin to address this disparity, we studied the transmission dynamics of the virus in Bangladesh during the country’s first COVID-19 wave by analysing case reports and whole-genome sequences from all eight divisions of the country. Results: We detected > 50 virus introductions to the country during the period, including during a period of national lockdown. Additionally, through discrete phylogeographic analyses, we identified that geographical distance and population -density and/or -size influenced virus spatial dispersal in Bangladesh. Conclusions: Overall, this study expands our knowledge of SARS-CoV-2 genomic epidemiology in Bangladesh, shedding light on crucial transmission characteristics within the country, while also acknowledging resemblances and differences to patterns observed in other nations.Published versio

Genomic epidemiology of early SARS-CoV-2 transmission dynamics in Bangladesh

Background: Genomic epidemiology has helped reconstruct the global and regional movement of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, there is still a lack of understanding of SARS-CoV-2 spread in some of the world’s least developed countries (LDCs). Methods: To begin to address this disparity, we studied the transmission dynamics of the virus in Bangladesh during the country’s first COVID-19 wave by analysing case reports and whole-genome sequences from all eight divisions of the country. Results: We detected > 50 virus introductions to the country during the period, including during a period of national lockdown. Additionally, through discrete phylogeographic analyses, we identified that geographical distance and population -density and/or -size influenced virus spatial dispersal in Bangladesh. Conclusions: Overall, this study expands our knowledge of SARS-CoV-2 genomic epidemiology in Bangladesh, shedding light on crucial transmission characteristics within the country, while also acknowledging resemblances and differences to patterns observed in other nations.ISSN:1743-422