Flow cytometric detection of gamma interferon can effectively discriminate Mycobacterium bovis BCG-vaccinated cattle from M. bovis-infected cattle

Mycobacterium bovis is the causative agent of bovine tuberculosis, a disease that is increasing in incidence in United Kingdom cattle herds. In addition to increasing economic losses, the rise in bovine tuberculosis poses a human health risk. There is an urgent requirement for effective strategies for disease eradication; this will likely involve vaccination in conjunction with current test and slaughter policies. A policy involving vaccination would require an accurate diagnosis of M. bovis-infected animals and the potential to distinguish these animals from vaccinates. Currently used diagnostic tests, the skin test and gamma interferon (IFN-γ) blood test, have a sensitivity of up to 95%. A further complication is that M. bovis BCG-vaccinated animals are also scored positive by these tests. We tested the hypothesis that the quantification of IFN-γ-producing lymphocytes by flow cytometric analysis of intracellular IFN-γ expression would provide a more accurate discrimination of M. bovis-infected animals from BCG vaccinates. Significant numbers of IFN-γ-expressing CD4(+) T cells were detected following culture of heparinized blood from M. bovis-infected animals, but not from BCG vaccinates, with purified protein derived from M. bovis (PPD-B) or live mycobacteria. Only 1 of 17 BCG-vaccinated animals had a significant number of CD4(+) T lymphocytes expressing IFN-γ, compared with 21/22 M. bovis-infected animals. This assay could allow an accurate diagnosis of M. bovis and allow the discrimination of BCG-vaccinated cattle from infected cattle

The Born and Markov approximations for atom lasers

We discuss the use of the Born and Markov approximations in describing the dynamics of an atom laser. In particular, we investigate the applicability of the quantum optical Born-Markov master equation for describing output coupling. We derive conditions based on the atomic reservoir, and atom dispersion relations for when the Born-Markov approximations are valid and discuss parameter regimes where these approximations fail in our atom laser model. Differences between the standard optical laser model and the atom laser are due to a combination of factors, including the parameter regimes in which a typical atom laser would operate, the different reservoir state which is appropriate for atoms, and the different dispersion relations between atoms and photons. We present results based on an exact method in the regimes in which the Born-Markov approximation fails. The exact solutions in some experimentally relavent parameter regimes give non-exponential loss of atoms from a cavity.Comment: 10 pages, 3 figures. (2 new figues). Exact solutions have been included in section II. Sections IV and V have been expanded. A new section discussing the effects of gravity has been include

Sequential Implementation of Monte Carlo Tests with Uniformly Bounded Resampling Risk

This paper introduces an open-ended sequential algorithm for computing the p-value of a test using Monte Carlo simulation. It guarantees that the resampling risk, the probability of a different decision than the one based on the theoretical p-value, is uniformly bounded by an arbitrarily small constant. Previously suggested sequential or non-sequential algorithms, using a bounded sample size, do not have this property. Although the algorithm is open-ended, the expected number of steps is finite, except when the p-value is on the threshold between rejecting and not rejecting. The algorithm is suitable as standard for implementing tests that require (re-)sampling. It can also be used in other situations: to check whether a test is conservative, iteratively to implement double bootstrap tests, and to determine the sample size required for a certain power.Comment: Major Revision 15 pages, 4 figure

Do early-life exposures explain why more advantaged children get eczema? Findings from the U.K. Millennium Cohort Study

Background: Atopic dermatitis (eczema) in childhood is socially patterned, with higher incidence in more advantaged populations. However, it is unclear what factors explain the social differences. Objectives: To identify early-life risk factors for eczema, and to explore how early-life risk factors explain any differences in eczema. Methods: We estimated odds ratios (ORs) for ever having had eczema by age 5 years in 14 499 children from the U.K. Millennium Cohort Study (MCS), with a focus on maternal, antenatal and early-life risk factors and socioeconomic circumstances (SECs). Risk factors were explored to assess whether they attenuated associations between SECs and eczema. Results: Overall 35·1% of children had ever had eczema by age 5 years. Children of mothers with degree-level qualifications vs. no educational qualifications were more likely to have eczema (OR 1·52, 95% confidence interval 1·31–1·76), and there was a gradient across the socioeconomic spectrum. Maternal atopy, breastfeeding (1–6 weeks and ≥ 6 months), introduction of solids under 4 months or cow's milk under 9 months, antibiotic exposure in the first year of life and grime exposure were associated with an increased odds of having eczema. Female sex, Pakistani and Bangladeshi ethnicity, smoking during pregnancy, exposure to environmental tobacco smoke and having more siblings were associated with reduced odds for eczema. Controlling for maternal, antenatal and early-life characteristics (particularly maternal smoking during pregnancy, breastfeeding and number of siblings) reduced the OR for eczema to 1·26 (95% confidence interval 1·03–1·50) in the group with the highest educational qualifications compared with the least. Conclusions: In a representative U.K. child cohort, eczema was more common in more advantaged children. This was explained partially by early-life factors including not smoking during pregnancy, breastfeeding and having fewer siblings

Rethinking Civic Engagement

Civic engagement is a key indicator of adulthood. Young adults respond to the social and political issues of the day in a variety of ways. After the police killings of George Floyd and Breonna Taylor in 2020, young people demonstrated against racial injustice in more than 10,000 peaceful protests around the country. That fall saw record numbers of youth turn out for the presidential election; half of eligible voters ages 18–29 participated, compared with 39 percent in 2016. Climate change likewise catalyzed young people, as nearly 30 percent of Generation Z and Millennials made donations, contacted public officials, volunteered, or protested, surpassing Generation X and Baby Boomers. Young people are commonly assumed to be disengaged, disillusioned, and uninterested in civic life. These trends challenge that proposition.Civic engage­ment is an import­ant part of our demo­cratic soci­ety, and it is a mean­ing­ful part of young people's healthy devel­op­ment and trans­ition into adult­hood. This report explores the concept of civic engage­ment and the distinct­ive, and some­times unac­coun­ted for, ways that young people parti­cip­ate in their communit­ies to improve social condi­tions, voice their needs and concerns, and uphold demo­cracy

The Economic Impact of Federal Land on County Governments in Utah

County governments cannot assess property taxes on federal land, yet local governments are required to provide similar services as they do on all other areas of the county. Federal government payment programs have been implemented to compensate county governments for the expenditures incurred due to federal land. In the mid-1960s, the Public Land Law Review Commission implemented and completed a study which analyzed whether selected individual states and counties were being compensated for the expenditures incurred on federal land. It also estimated tax revenues local governments would receive if federally owned acreage was privately owned. The study then compared these potential revenues with existing revenues from government payment programs. The purpose of this study was to identify net revenues from county government expenditures and revenues due to federally owned land for the years 1975 through 1990. Comparisons were also made between estimated tax revenues, if federal land acreage was privately owned, and federal land-related government payment programs. Two Utah counties, Box Elder and Kane, were selected for this study. County government audit reports and other county records, along with information and data obtained from county and federal government personnel, were obtained and analyzed. Comparisons were made between these findings and the Public Land Law Review Commission mid-1960s results and conclusions. The results are opposite between the two counties and from the Public Land Law Review Commission Study