6 research outputs found
Crystal samples from 800 m visualized by light microscopy.
<p>(A) A salt crystal chiseled off from the surface sterilized isolation sample. (B) A magnification of a subsection of (A), showing liquid inclusions inside the crystal. (C) Cubic liquid inclusions. Bars represent 0.5 mm in A and C, and 0.1 mm in B.</p
Microbes isolated from rock salt drill core sample from the depth of 800 m.
<p>(A) The whole cell protein analysis of the isolates by SDS-PAGE. The numbers refer to strains YI80-1 to YI80-8. Molecular mass markers are shown on left. (B) Light microscopy pictures of the isolates in late stationary phase. The scale bar in YI80-1 represents 10 µm, and is applicable to all pictures. (C) Phenotypic characteristics of the isolates.</p
Sampling location and the drill core sample.
<p>(A) The map of Yunying Depression, with the sampling site, Yingcheng (30° 55′ N, 113°34′ E), bolded and marked with a black dot. (B) Subsections of a drill core from the site. (C) The 600 m (D) and the 800 m samples, of which subsections were used for microbe isolation in this work. The bars in C and D represent 5 cm.</p
Maximum likelihood phylogenetic tree of the 16S rRNA gene sequences.
<p>GenBank numbers are given in brackets. <i>Halolamina</i> species are marked with a pink background, and species of <i>Halobacterium</i> with light blue one. Strains isolated from the 800 m rock salt sample are bolded, and partial sequences marked with an asterisk (*). Bar represents inferred substitutions per nucleotide position. <i>Methanospirillum hungatei</i> is used as an outgroup.</p