Low FOXD3 expression was assotiated with poor prognosis in HGG patients.

<p>A and B: Kaplan-Meier curves OS and PFS in all HGG patients according to the expression levels of FOXD3, respectively. C and D: Kaplan-Meier curves OS and PFS in grade III glioma patients according to the expression levels of FOXD3, respectively. E and F: Kaplan-Meier curves OS and PFS in grade IV glioma patients according to the expression levels of FOXD3, respectively.</p

FOXD3 expression in HGG tissues and glioma cell lines.

<p>A: FOXD3 mRNA expression in 23 HGG cases and 13 control tissues. B: FOXD3 protein expression in 23 HGG cases and 13 control tissues. C: Representative protein expression of FOXD3 in 7 HGG cases and 5 control tissues by western blotting. D and E: Representative immunohistochemical staining of high expression and low FOXD3 expression in HGG cases, respectively. F and G: FOXD3 expression in five glioma cell lines by western blotting. Scale bar, 50μm.</p

Relationship between FOXD3 expression and clinicopathological features of 184 patients with HGGs.

<p>Abbreviations: GTR, gross total removal; KPS score, Karnofsky performance score; NTR, near total removal.</p><p>Relationship between FOXD3 expression and clinicopathological features of 184 patients with HGGs.</p

FOXD3 silencing promoted proliferation and inhibited starvation-induced apoptosis in SW1080 cells.

<p>A: Western blotting analysis of FOXD3 and cleaved caspase-3 expression in SW1080 cells. B: Cell proliferation assay showing significantly enhanced proliferation rate of FOXD3-silenced SW1080 cells in comparison with siNC-treated SW1080 cells (<i>P</i><0.05) by MTT assay. C: Representative images of EdU assay in SW1080 cells. D: Statistical results from five independent EdU assays. E: Representative results of apoptosis assay by flow cytometry in serum-starvated SW1080 cells. F: Statistical results from three independent apoptosis assays. Summarized results are expressed as mean ±SD. EV, empy vector; NC, negative control. Scale bar, 50μm.</p

FOXD3 overexpression suppressed proliferation and enhanced starvation-induced apoptosis in U87MG cells.

<p>A: Western blotting analysis of FOXD3 and cleaved caspase-3 expression in U87MG cells. B: Cell proliferation assay showing significantly enhanced proliferation rate of FOXD3-overexpressed U87MG cells in comparison with EV-treated U87MG cells (<i>P</i><0.05) by MTT assay. C: Representative images of EdU assay in U87MG cells. D: Statistical results from five independent EdU assays. E: Representative results of apoptosis assay by flow cytometry in serum-starvated U87MG cells. F: Statistical results from three independent apoptosis assays. Summarized results are expressed as mean ±SD. EV, empy vector; NC, negative control. Scale bar, 50μm.</p