Aerogel Microspheres from Natural Cellulose Nanofibrils and Their Application as Cell Culture Scaffold

We demonstrated that ultralight pure natural aerogel microspheres can be fabricated using cellulose nanofibrials (CNF) directly. Experimentally, the CNF aqueous gel droplets, produced by spraying and atomizing through a steel nozzle, were collected into liquid nitrogen for instant freezing followed by freeze-drying. The aerogel microspheres are highly porous with bulk density as low as 0.0018 g cm^–3. The pore size of the cellulose aeogel microspheres ranges from nano- to macrometers. The unique ultralight and high porous structure ensured high moisture (∼90 g g^–1) and water uptake capacity (∼100 g g^–1) of the aerogel microspheres. Covalent cross-linking between the native nanofibrils and cross-linkers made the aerogel microspheres very stable even in a harsh environment. The present study also confirmed this kind of aerogel microspheres from native cellulose fibers can be used as cell culture scaffold