Correlation analysis among the toxicity of the 4 heavy metals to the 3 Cyprinid Fish species.

**<p>Correlation is significant at the 0.01 level (2-tailed).</p

Analysis of the acute toxicity, respiratory reaction and sensitivity of different fish species.

<p>Analysis of the acute toxicity, respiratory reaction and sensitivity of different fish species.</p

LC₅₀ values (mgL^−<b>1</b>) over time for zebrafish (<i>Brachydanio rerio)</i>, rare Minnow <i>(Gobiocypris rarus)</i> and juvenile grass carp <i>(Ctenopharyngodon idellus)</i> exposed to 4 kinds of heavy metals.

<p>LC₅₀ values (mgL^−<b>1</b>) over time for zebrafish (<i>Brachydanio rerio)</i>, rare Minnow <i>(Gobiocypris rarus)</i> and juvenile grass carp <i>(Ctenopharyngodon idellus)</i> exposed to 4 kinds of heavy metals.</p

Ventilatory responses over time for zebrafish (<i>Brachydanio rerio)</i>, rare minnow <i>(Gobiocypris rarus)</i> and juvenile grass carp <i>(Ctenopharyngodon idellus)</i> exposed to 4 kinds of heavy metals.

<p>Ventilatory responses over time for zebrafish (<i>Brachydanio rerio)</i>, rare minnow <i>(Gobiocypris rarus)</i> and juvenile grass carp <i>(Ctenopharyngodon idellus)</i> exposed to 4 kinds of heavy metals.</p

Rab27a expression was significantly different for different TCGA and CGGA subtypes and IDH1 mutation status.

<p>A (p<0.01), D (p<0.01), and F (p<0.01) indicate that Rab27a has a mesenchymal preference. B (p<0.01) and E (p<0.01) indicate that Rab27a has a G3 preference. C (p<0.01) indicates that patients with wild type IDH1 also had higher Rab27a expression compared to patients with mutations in the IDH1 gene. A single spot represents the Rab27a expression value of an individual patient, and the lines in the middle represent the mean expression value. The difference in Rab27a expression was tested by the Student t-test.</p

Rab27a protein expression was correlated with glioma grade progression and prognosis.

<p>Immunohistochemical staining showed that Rab27a expression increased with grade in gliomas. The sections were counterstained with hematoxylin and mounted with Permount. The immunochemistry scoring system was as follows (A): normal brain tissue: 0 (a), astrocytoma: 1 (WHO Grade II) (b), anaplastic astrocytoma: 4 (WHO Grade III) (c), and glioblastoma: 9 (WHO Grade IV) (d). (B) Histogram showed staining score increased from normal to WHO Grade IV. (C) By kaplan meier analysis including 96 GBMs, we found high Rab27b protein expression harbored significant short overall survival.</p

Clinical characteristics of 220 glioma patients.

<p>Karnofsky Performance Scale (KPS).</p

Rab27a expression showed mesenchymal, G3 subtype, and IDH1 wild-type preferences.

<p>For each patient, the TCGA and CGGA subtypes are annotated as previously reported and are listed in the upper part together with the IDH1 mutation status, which was obtained from the CGGA database. Correlated genes are supervised by clustering according to Rab27a expression, from low to high. The positively and negatively correlated genes are shown in the lower panel (marked pink and blue on the right, respectively). NA, not available.</p

Gene sets enriched in WHO Grade IV glioma samples with high Rab27A expression.

<p>Gene sets enriched in WHO Grade IV glioma samples with high Rab27A expression.</p

Gene set variation analysis with Rab27a expression.

<p>Gene set variation analysis with Rab27a expression was analyzed using the GSVA package. Gene expression signatures of migration were generated from the GO list. Rab27a expression is listed from left to right, starting with the highest expression level. A high enrichment score indicates a positive correlation with Rab27a expression, and a low enrichment score indicates the reverse.</p