Stackelberg game-based three-stage optimal pricing and planning strategy for hybrid shared energy storage

Inspired from sharing economy and advanced energy storage technologies, hybrid shared energy storage (HSES), as an innovative business model, can provide flexible storage leasing services to new energy stations (NESs) and bring additional profits to the energy storage owner. Under this business model, pricing and planning issues are the main focus of the HSES operator to increase revenues but are rarely considered in current studies. Therefore, a Stackelberg game-based three-stage optimal pricing and planning strategy of HSES is formulated for the operator. First, an HSES model considering two leasing options is developed to provide two kinds of short-term use rights of energy storage resources for NESs. Then, the interactions between selfish NESs and the HSES operator are characterized as a Stackelberg game, and a bi-level pricing and planning strategy optimization model is developed to help the HSES operator make optimal decisions. Finally, considering different characteristics in each stage of the Stackelberg game, a three-stage solution method based on the genetic algorithm (GA) and mixed-integer linear programming (MILP) models is proposed to solve the optimization problem. Case studies on six NESs in a certain region are taken to verify the effectiveness of the proposed strategy. Simulation results show that the HSES operator can obtain maximum profit under the proposed pricing and planning strategy. In addition, the proposed HSES leasing model can provide additional benefits to both the operator and NESs

Licarin-B Exhibits Activity Against the Toxoplasma gondii RH Strain by Damaging Mitochondria and Activating Autophagy

Toxoplasma gondii is an obligate intracellular pathogen that infects warm-blooded animals and humans. However, side effects limit toxoplasmosis treatment, and new drugs with high efficiency and low toxicity need to be developed. Natural products found in plants have become a useful source of drugs for toxoplasmosis. In this study, twenty natural compounds were screened for anti-T. gondii activity by Giemsa staining or real-time fluorescence quantitative polymerase chain reaction (qPCR) in vitro. Among these, licarin-B from nutmeg exhibited excellent anti-T. gondii activity, inhibiting T. gondii invasion and proliferation in a dose-dependent manner, with an EC50 of 14.05 ± 3.96 μg/mL. In the in vivo, licarin-B treatment significantly reduced the parasite burden in tissues compared to no treatment, protected the 90% infected mice from to death at 50 mg/kg.bw. Flow cytometry analysis suggested a significant reduction in T. gondii survival after licarin-B treatment. Ultrastructural changes in T. gondii were observed by transmission electron microscopy (TEM), as licarin-B induced mitochondrial swelling and formation of cytoplasmic vacuoles, an autophagosome-like double-membrane structure and extensive clefts around the T. gondii nucleus. Furthermore, MitoTracker Red CMXRos, MDC, and DAPI staining showed that licarin-B promoted mitochondrial damage, autophagosome formation, and nuclear disintegration, which were consistent with the TEM observations. Together, these findings indicate that licarin-B is a promising anti-T. gondii agent that potentially functions by damaging mitochondria and activating autophagy, leading to T. gondii death

Characterization of a Metagenome-Derived β-Glucosidase and Its Application in Conversion of Polydatin to Resveratrol

For the beneficial pharmacological properties of resveratrol, there is increasingly interest in enzymatic conversion of polydatin to resveratrol. The metagenomic technique provides an effective strategy for mining novel polydatin-hydrolysis enzymes from uncultured microorganisms. In this study, a metagenomic library of mangrove soil was constructed and a novel β-glucosidase gene MlBgl was isolated. The deduced amino acid sequences of MlBgl showed the highest identity of 64% with predicted β-glucosidase in the GenBank database. The gene was cloned and overexpressed in Escherichia coli BL21(DE3). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay demonstrated the purified recombinant β-glucosidase r-MlBgl with a molecular weight approximately of 71 kDa. The optimal pH and temperature of purified recombinant r-MlBgl were 7.0 and 40 °C, respectively. r-MlBgl could hydrolyze polydatin effectively. The kcat and kcat/Km values for polydatin were 989 s−1 and 1476 mM−1·s−1, respectively. These properties suggest that -r-MlBgl has potential application in the enzymatic conversion of polydatin to resveratrol for further study

Isolation and Characterization of a Glycosyl Hydrolase Family 16 β-Agarase from a Mangrove Soil Metagenomic Library

A mangrove soil metagenomic library was constructed and a β-agarase gene designated as AgaML was isolated by functional screening. The gene encoded for a 659-amino-acids polypeptide with an estimated molecular mass of 71.6 kDa. The deduced polypeptide sequences of AgaML showed the highest identity of 73% with the glycoside hydrolase family 16 β-agarase from Microbulbifer agarilyticus in the GenBank database. AgaML was cloned and highly expressed in Escherichia coli BL21(DE3). The purified recombinant protein, AgaML, showed optimal activity at 50 °C and pH 7.0. The kinetic parameters of Km and Vmax values toward agarose were 4.6 mg·mL−1 and 967.5 μM·min−1·mg−1, respectively. AgaML hydrolyzed the β-1,4-glycosidic linkages of agar to generate neoagarotetraose (NA4) and neoagarohexaose (NA6) as the main products. These characteristics suggest that AgaML has potential application in cosmetic, pharmaceuticals and food industries

Study on the Photothermal Performance of a “Thermal Shielding” Coating Using Tungsten Bronze as Functional Material for Asphalt Pavement

Asphalt pavements absorb more than 90% of the incident solar radiation, which induces not only high-temperature degradation but also the urban heat island (UHI) effect. In this study, a novel nanoscale non-stoichiometric compound containing tungsten (MxWO3) was used for the first time to prepare thermal shielding coatings to reduce the temperature of pavements and mitigate the UHI effect. Coatings with good shielding characteristics were selected for outdoor thermal insulation tests to evaluate their properties. MxWO3 (M = K, Na, Cs) exhibited significant thermal shielding, especially CsxWO3. Outdoor thermal insulation tests were performed for the CsxWO3 coatings, and it was found that the greater the doping, the more significant the thermal shielding effect. Compared with untreated pavements, the surface-coated pavement exhibited significant cooling at 5 cm and 15 cm depth-wise, which reduced the overall pavement temperature by 1–2 °C, and the coating thickness affected the cooling effect

Foraging Niche Differentiation of Five Woodpecker Species in the Primitive Broadleaved Korean Pine Forests of Northeast China

Explaining the mechanism of the coexistence of sympatric species is an important goal of ecology. Five species of woodpeckers coexist in the broadleaved Korean pine forest of Liangshui National Nature Reserve, including the Black Woodpecker (Dryocopus martius), Great Spotted Woodpecker (Dendrocopos major), Lesser Spotted Woodpecker (Dendrocopos minor), Three-toed Woodpecker (Picoides tridactylus), and White-backed Woodpecker (Dendrocopos leucotos). Woodpeckers are considered to be keystone species because of their role as ecosystem engineers, creating breeding and shelter sites for many vertebrate and invertebrate taxa. As woodpeckers are predominant in primary forests, they are sensitive to changes in forest ecosystems. To understand their coexistence mechanisms and propose conservation strategies, it is necessary to investigate their foraging niche differentiation. This study aimed to identify the foraging behavior parameters and foraging tree parameters of five woodpecker species in Liangshui Reserve from October to December. The foraging niches of five woodpecker species were observed, including the type of foraging techniques, foraging height, foraging site, foraging duration, tree species being foraged upon, diameter of the foraging tree at breast height, foraging tree height, and decay status of trees. Our results identified that there were significant differences in the overall foraging ecology of the five species of woodpecker at Liangshui Reserve. The Great Spotted Woodpecker and Lesser Spotted Woodpecker had more diverse foraging patterns and preferred to forage on live trees. The Black Woodpecker and Three-toed Woodpecker excavated and pecked at the trunks of decaying spruce and fir trees. The White-backed Woodpecker preferred to forage in broadleaved trees. The choice of foraging sites was complicated. The size of the foraging trees and decay status of trees were important bases for woodpeckers when choosing trees to forage from. Different woodpeckers achieve stable coexistence through the separation of their foraging niches. This information regarding foraging behavior and foraging tree characteristics provides a basis to study the coexistence patterns of woodpeckers. Our research into woodpecker foraging should be used to inform forest management practices, protect forest ecosystem diversities, and maintain woodpecker community diversity

Licochalcone A: An effective and low-toxicity compound against Toxoplasma gondii in vitro and in vivo

Toxoplasma gondii, an obligate intracellular protozoan, is the causative agent of toxoplasmosis, which can cause serious public health problems. The current drugs used to treat toxoplasmosis have many limitations. This study evaluated the anti-T. gondii activity and potential mechanism of Licochalcone A (Lico A) in vitro and in vivo. The safe concentration of Lico A in HFF cells was determined by MTT cell viability assays. The presence of T. gondii was assessed by qPCR and Giemsa staining. Azithromycin and sulfadiazine, commonly used effective treatments, served as drug controls. T. gondii ultrastructural alterations were observed by electron microscopy. The anti-T. gondii activity of Lico A was evaluated using an in vivo mouse infection model. In vitro, Lico A had no negative effect on host cell viability at concentrations below 9 μg/mL; however, it did inhibit T. gondii proliferation in a dose-dependent manner, with a 50% inhibitory concentration (IC50) of 0.848 μg/mL. Electron microscopy analyses indicated substantial structural and ultrastructural changes in tachyzoites after Lico A treatment. Nile Red staining assays demonstrated that Lico A caused lipid accumulation. Lico A treatment significantly increased the survival rate of BALB/c mice infected with T. gondii. Lico A achieved the same therapeutic effect as a commonly used clinical drugs (combination of sulfadiazine, pyrimethamine and folinic acid). In conclusion, Lico A has strong anti-T. gondii activity in vitro and in vivo and might be developed into a new anti-T. gondii drug. Moreover, Lico A may exert these effects by interfering with lipid metabolism in the parasite. Keywords: Licochalcone A, Anti-Toxoplasma gondii, Ultrastructural, Cytotoxicity, Survival curv

Preparation of Monoclonal Antibody against Pyrene and Benzo [a]pyrene and Development of Enzyme-Linked Immunosorbent Assay for Fish, Shrimp and Crab Samples

Polycyclic aromatic hydrocarbons (PAHs) are significant environmental and food pollutants that can cause cancer. In this work, a specific monoclonal antibody (mAb) to identify pyrene (PYR) and benzo [a]pyrene (BaP) was prepared, and an indirect competitive enzyme-linked immunoassay (ic-ELISA) was established to detect PYR and BaP residues in living aquatic products for the first time. The effects of complete antigens with different coupling ratios on the production of high-sensitivity mAb was explored. Under the optimal conditions, the IC50 value was 3.73 ± 0.43 µg/L (n = 5). The limits of detection (LODs) for PYR and BaP in fish, shrimp, and crab ranged from 0.43 to 0.98 µg/L. The average recoveries of the spiked samples ranged from 81.5–101.9%, and the coefficient of variation (CV) was less than 11.7%. The validation of the HPLC-FLD method indicated that the ELISA method set up in this experiment provided a trustworthy tool for PAHs residues detection in aquatic products