Differential miRNAs expression in gene array and Q-PCR.

<p>Differential miRNAs expression in gene array and Q-PCR.</p

Validated targeted genes for defferentially-expressed miRNAs.

<p>Validated targeted genes for defferentially-expressed miRNAs.</p

The effect of acarbose on oral glucose tolerance test blood glucose (A) and AUC (B) in rats.

<p>Data represent mean ± SD (n = 10 per group). **<i>P</i><0.01 versus the control group; ^#<i>P</i><0.05 versus DM group.</p

Multivariate stepwise linear regression analysis with Ln insulin sensitivity index (ISI_OGTT) as dependent variable.

<p>Independent variables included in the model were pre-BMI, gestational age, systolic blood pressure, triglycerides, SPARC, adiponectin, fasting proinsulin, hsCRP, WBC count and ALT. ^a Skewed distributions were logarithmically transformed for analysis. ISI_OGTT: insulin sensitivity index; hsCRP, hypersensitive C reaction protein; TG, triglyceride; SPARC, secreted protein acidic and rich in cysteine.</p

Differentially expressed miRNA (fold change>2, <i>P</i><0.05).

<p>Differentially expressed miRNA (fold change>2, <i>P</i><0.05).</p

The Volcano Plot graph of miRNA array.

<p>This graph shows that log 2 of the fold change in each miRNA’s expression between AcarH group and DM group is versus its -log 10 of <i>P</i> value from the <i>t</i>-test. The vertical green line indicate that the fold change in miRNA expression threshold is 2. The horizonal green lin indicates that the <i>P</i> value of the <i>t</i>-test threshold is 0.05. There were 8 miRNAs which showed significantly different expression between AcarH group and DM group.</p

Body weight (A) and fasting blood glucose (B) before and after acarbose treatment in rats.

<p>Data represent mean ± SD (n = 10 per group). **<i>P</i><0.01 versus the control group; ^#<i>P</i><0.05 versus DM group.</p

Validated target genes grouped by KEGG pathway.

<p>Validated target genes grouped by KEGG pathway.</p

The effect of acarbose on serum IL6 (A) and TNF-α (B) in rats (n = 10 per group).

<p>Data represent mean ± SD (n = 10). **<i>P</i><0.01 versus the control group; ^##<i>P</i><0.01 versus DM group.</p

Olgonucleotide sequences for mRNA Q-PCR.

<p><i>Il6</i>: interleukin 6; <i>Mapk1</i>: mitogen activated protein kinase 1; <i>Tnf</i>: tumor necrosis factor; <i>Gadph</i>: glyceraldehyde-3-phosphate dehydrogenase.</p