Regiospecific liver repopulation in DPPIV⁻ recipients by donor DPPIV⁺ hepatocytes following conformal HIR of one half of the median lobe.

<p>All liver lobes, except one half of the median lobe were shielded from HIR. Three months after hepatocyte transplantation, liver sections were stained histochemically for DPPIV activity. A region at the junction of the irradiated and non-irradiated regions of the median lobe is shown. Panel a. Low power view showing clusters of DPPIV-positive donor hepatocytes (red, white arrow) in the upper part of the section that had received preparative HIR, and the absence of such clusters in the lower part of the section that had been shielded from HIR. Panel b. High power view showing a donor hepatocyte cluster. The black bars indicate 100 µm.</p

Ugt1a1 activity toward bilirubin in Gunn rats that received conformal HIR before hepatocyte transplantation.

<p>Ugt1a1 activities in liver homogenates of different recipient groups as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046775#pone-0046775-g002" target="_blank">Figure 2</a> are shown (means±SD) as percent of the activity in the liver of Wistar-RHA rats (2.6 µmol/g liver per hour).</p

Immunofluorescent staining for ugt1a1 in different liver lobes.

<p>Three months after hepatocyte transplantation following HIR to median lobe (ML), 20% of the irradiated median lobe was replaced by donor hepatocytes, whereas the unirradiated left (LL), right (RL) and caudate (CL) lobes contained only small ugt1a1+ hepatocyte clusters.</p