Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major)

Primordial germ cells (PGCs) are the precursors of gametes responsible for genetic transmission to the next generation. They provide an ideal system for cryopreservation and restoration of biodiversity. Recently, considerable attention has been raised to visualize, isolate and transplant PGCs within and between species. In fish, stable PGC visualization in live embryo and individual has been limited to laboratory fish models such as medaka and zebrafish. One exception is the rainbow trout, which represents the only species with aquaculture importance and has GFP-labeled germ cells throughout development. PGCs can be transiently labeled by embryonic injection of mRNA containing green fluorescence protein gene (GFP) and 3'-untranslated region (3'-UTR) of a maternal germ gene such as vasa, nos1, etc. Stable PGC labeling can be achieved through production of transgenic animals by some transcriptional regulatory sequences from germ genes, such as the vasa promoter and 3'-UTR. In this study, we reported the functional analyses of the red seabream vasa (Pmvas) regulatory sequences, using medaka as a model system. It was showed that injection of GFP-Pmvas3'UTR mRNA was able to label medaka PGCs during embryogenesis. Besides, we have constructed pPmvasGFP transgenic vector, and established a stable transgenic medaka line exhibiting GFP expression in germ cells including PGCs, mitotic and meiotic germ cells of both sexes, under control of the Pmvas transcriptional regulatory sequences. It is concluded that the Pmvas regulatory sequences examined in this study are sufficient for germ cell expression and labeling

Construction of stable Ta3N5/g-C3N4 metal/non-metal nitride hybrids with enhanced visible-light photocatalysis

In this paper, a novel Ta3N5/g-C3N4 metal/non-metal nitride hybrid was successfully synthesized by a facile impregnation method. The photocatalytic activity of Ta3N5/g-C3N4 hybrid nitrides was evaluated by the degradation of organic dye rhodamine B (RhB) under visible light irradiation, and the result indicated that all Ta3N5/g-C3N4 samples exhibited distinctly enhanced photocatalytic activities for the degradation of RhB than pure g-C3N4. The optimal Ta3N5/g-C3N4 composite sample, with Ta3N5 mass ratio of 2%, demonstrated the highest photocatalytic activity, and its degradation rate constant was 2.71 times as high as that of pure g-C3N4. The enhanced photocatalytic activity of this Ta3N5/g-C3N4 metal/metal-free nitride was predominantly attributed to the synergistic effect which increased visible-light absorption and facilitated the efficient separation of photoinduced electrons and holes. The Ta3N5/g-C3N4 hybrid nitride exhibited excellent photostability and reusability. The possible mechanism for improved photocatalytic performance was proposed. Overall, this work may provide a facile way to synthesize the highly efficient metal/metal-free hybrid nitride photocatalysts with promising applications in environmental purification and energy conversion

Involvement of TRPC Channels in Lung Cancer Cell Differentiation and the Correlation Analysis in Human Non-Small Cell Lung Cancer

The canonical transient receptor potential (TRPC) channels are Ca2+-permeable cationic channels controlling the Ca2+ influx evoked by G protein-coupled receptor activation and/or by Ca2+ store depletion. Here we investigate the involvement of TRPCs in the cell differentiation of lung cancer. The expression of TRPCs and the correlation to cancer differentiation grade in non-small cell lung cancer (NSCLC) were analyzed by real-time PCR and immunostaining using tissue microarrays from 28 patient lung cancer samples. The association of TRPCs with cell differentiation was also investigated in the lung cancer cell line A549 by PCR and Western blotting. The channel activity was monitored by Ca2+ imaging and patch recording after treatment with all-trans-retinoic acid (ATRA). The expression of TRPC1, 3, 4 and 6 was correlated to the differentiation grade of NSCLC in patients, but there was no correlation to age, sex, smoking history and lung cancer cell type. ATRA upregulated TRPC3, TRPC4 and TRPC6 expression and enhanced Ca2+ influx in A549 cells, however, ATRA showed no direct effect on TRPC channels. Inhibition of TRPC channels by pore-blocking antibodies decreased the cell mitosis, which was counteracted by chronic treatment with ATRA. Blockade of TRPC channels inhibited A549 cell proliferation, while overexpression of TRPCs increased the proliferation. We conclude that TRPC expression correlates to lung cancer differentiation. TRPCs mediate the pharmacological effect of ATRA and play important roles in regulating lung cancer cell differentiation and proliferation, which gives a new understanding of lung cancer biology and potential anti-cancer therapy. © 2013 Jiang et al

AoI-Minimal Online Scheduling for Wireless Powered IoT:A Lyapunov Optimization-based Approach

This paper investigates the age of information (AoI)-based online scheduling in multi-sensor wireless powered communication networks (WPCNs) for time-sensitive Internet of Things (IoT). Specifically, we consider a typical WPCN model, where a wireless power station (WPS) charges multiple sensor nodes (SNs) by wireless power transfer (WPT), and then the SNs are scheduled in the time domain to transmit their sampled status information with their harvested energy to a mobile edge server (MES) for decision making. For such a system, we first derive a closed-form expression of the successful data transmission probability in Nakagami-m fading channels. To pursue an efficient online scheduling policy that minimizes the Expected Weighted Sum AoI (EWSAoI) of the system, a discrete-time scheduling problem is formulated. As the problem is non-convex with non-explicit expression of the EWSAoI, we propose a Max-Weight policy based on the Lyapunov optimization theory, which schedules the SNs at the beginning of each time in terms of the one-slot conditional Lyapunov Drift. Simulations demonstrate our presented theoretical results and show that our proposed scheduling policy outperforms other baselines such as greedy policy and random round-robin (RR) policy. Especially, when the number of SNs is relatively small, the gain achieved by the proposed policy compared to the greedy policy is considerable. Moreover, some interesting insights are also observed: 1) as the number of SNs increases, the EWSAoI also increases; 2) when the transmit power is relatively small, the larger the number of SNs, the smaller the EWSAoI; 3) the EWSAoI decreases with the increment of transmit power of the WPS and then tends to be flat; 4) the EWSAoI increases with the increment of the distance between the SNs and the MES

Burden of dilated perivascular spaces in patients with moyamoya disease and moyamoya syndrome is related to middle cerebral artery stenosis

Background and objectiveThe correlation between intracranial large artery disease and cerebral small vessel disease (CSVD) has become a noteworthy issue. Dilated perivascular spaces (dPVS) are an important marker of CSVD, of which cerebral atrophy has been regarded as one of the pathological mechanisms. DPVS has been found to be associated with vascular stenosis in patients with moyamoya disease (MMD), but the underlying mechanism remains unclear. The purpose of our study was to explore the correlation between the middle cerebral artery (MCA) stenosis and dPVS in the centrum semiovale (CSO-dPVS) in patients with MMD/moyamoya syndrome (MMS) and to determine whether brain atrophy plays a mediating role in this relationship.MethodsA total of 177 patients were enrolled in a single-center MMD/MMS cohort. Images of their 354 cerebral hemispheres were divided into three groups according to dPVS burden: mild (dPVS 0–10), moderate (dPVS 11–20), and severe (dPVS > 20). The correlations among cerebral hemisphere volume, MCA stenosis, and CSO-dPVS were analyzed, adjusting for the confounding factors of age, gender, and hypertension.ResultsAfter adjustment for age, gender, and hypertension, the degree of MCA stenosis was independently and positively associated with ipsilateral CSO-dPVS burden (standardized coefficient: β = 0.247, P < 0.001). A stratified analysis found that the subgroup with a severe CSO-dPVS burden exhibited a significantly higher risk of severe stenosis of the MCA [p < 0.001, OR = 6.258, 95% CI (2.347, 16.685)]. No significant correlation between CSO-dPVS and ipsilateral hemisphere volume was found (p = 0.055).ConclusionIn our MMD/MMS cohort, there was a clear correlation between MCA stenosis and CSO-dPVS burden, which may be a direct effect of large vessel stenosis, without a mediating role of brain atrophy

Dwarf Galaxy Discoveries from the KMTNet Supernova Program III. the Milky-Way Analog NGC~2997 Group

We present the discovery of 48 new and the analysis of 55, including 7 previously discovered, dwarf galaxy candidates around the giant spiral galaxy NGC~2997 using deep $BVI$ images from the KMTNet Supernova Program. Their $V$-band central surface brightness and total absolute magnitudes range between 20.3--26.7 mag arcsec$^{-2}$ and --(8.02--17.69) mag, respectively, while the $I$-band effective radii are between 0.14 and 2.97 kpc. We obtain $\alpha$ $\simeq$ --1.43 $\pm$ 0.02 for the faint-end slope of their luminosity function, comparable to previously measured values but shallower than theoretical predictions based on $\Lambda$CDM models. The distance-independent distributions of their mass and color from the host galaxy NGC~2997 suggest that the group could be dynamically young, prior to the development of significant mass segregation or radial color gradients. The systematically bluer colors of the brighter candidates than the fainter ones indicate higher star formation activities in brighter members. We suggest that the higher-mass dwarf galaxies in the group have maintained star-formation activities by effectively retaining gas content, while environmental quenching is only effective for the lower-mass galaxies. The interpretation of early evolutionary stage of this group is also consistent with the overall morphological distribution of the dwarf galaxy candidates showing a lack of morphologically evolved candidates but a plethora of irregularly shaped ones. Our detection rate of dwarf galaxy candidates in the NGC~2997 group and their inferred star formation activities are largely comparable to those found in Milky Way analog systems from the SAGA survey within the magnitude limit M$_{V}$ $\lesssim$ --13 mag, as well as those found in the ELVES survey

Enhancement of Canonical Wnt/β-Catenin Signaling Activity by HCV Core Protein Promotes Cell Growth of Hepatocellular Carcinoma Cells

BACKGROUND: The Hepatitis C virus (HCV) core protein has been implicated as a potential oncogene or a cofactor in HCV-related hepatocellular carcinoma (HCC), but the underlying mechanisms are unknown. Overactivation of the Wnt/β-catenin signaling is a major factor in oncogenesis of HCC. However, the pathogenesis of HCV core-associated Wnt/β-catenin activation remains to be further characterized. Therefore, we attempted to determine whether HCV core protein plays an important role in regulating Wnt/β-catenin signaling in HCC cells. METHODOLOGY: Wnt/β-catenin signaling activity was investigated in core-expressing hepatoma cells. Protein and gene expression were examined by Western blot, immunofluorescence staining, RT-qPCR, and reporter assay. PRINCIPAL FINDINGS: HCV core protein significantly enhances Tcf-dependent transcriptional activity induced by Wnt3A in HCC cell lines. Additionally, core protein increases and stabilizes β-catenin levels in hepatoma cell line Huh7 through inactivation of GSK-3β, which contributes to the up-regulation of downstream target genes, such as c-Myc, cyclin D1, WISP2 and CTGF. Also, core protein increases cell proliferation rate and promotes Wnt3A-induced tumor growth in the xenograft tumor model of human HCC. CONCLUSIONS/SIGNIFICANCE: HCV core protein enhances Wnt/β-catenin signaling activity, hence playing an important role in HCV-associated carcinogenesis

Integrated powered density: Screening ultrahigh dimensional covariates with survival outcomes

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/144590/1/biom12820.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/144590/2/biom12820_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/144590/3/biom12820-sup-0001-SuppData.pd