Parasitoid Increases Survival of Its Pupae by Inducing Hosts to Fight Predators

Many true parasites and parasitoids modify the behaviour of their host, and these changes are thought to be to the benefit of the parasites. However, field tests of this hypothesis are scarce, and it is often unclear whether the host or the parasite profits from the behavioural changes, or even if parasitism is a cause or consequence of the behaviour. We show that braconid parasitoids (Glyptapanteles sp.) induce their caterpillar host (Thyrinteina leucocerae) to behave as a bodyguard of the parasitoid pupae. After parasitoid larvae exit from the host to pupate, the host stops feeding, remains close to the pupae, knocks off predators with violent head-swings, and dies before reaching adulthood. Unparasitized caterpillars do not show these behaviours. In the field, the presence of bodyguard hosts resulted in a two-fold reduction in mortality of parasitoid pupae. Hence, the behaviour appears to be parasitoid-induced and confers benefits exclusively to the parasitoid

Primary rat sertoli and interstitial cells exhibit a differential response to cadmium

Two cell types central to the support of spermatogenesis, the Sertoli cell and the interstitial (Leydig) cell, were isolated from the same cohort of young male rats and challenged with cadmium chloride to compare their susceptibility to the metal. Both cell types were cultured under similar conditions, and similar biochemical endpoints were chosen to minimize experimental variability. These endpoints include the uptake of 109 Cd, reduction of the vital tetrazolium dye MTT, incorporation of 3 H-leucine, change in heat-stable cadmium binding capacity, and production of lactate. Using these parameters, it was observed that the Sertoli cell cultures were adversely affected in a dose-and time-dependent manner, while the interstitial cell cultures, treated with identical concentrations of CdCl 2 , were less affected. The 72-hr LC 50 's for Sertoli cells and interstitial cells were 4.1 and 19.6 μM CdCl 2 , respectively. Thus, different cell populations within the same tissue may differ markedly in susceptibility to a toxicant. These in vitro data suggest that the Sertoli cell, in relation to the interstitium, is particularly sensitive to cadmium. Because the Sertoli cell provides functional support for the seminiferous epithelium, the differential sensitivity of this cell type may, in part, explain cadmium-induced testicular dysfunction, particularly at doses that leave the vascular epithelium intact.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42554/1/10565_2004_Article_BF00135027.pd

Benchmark study on fuel bundle degradation in the phebus FPT2 test using state-of-the-art severe accident analysis codes

This paper presents the results of posttest calculations of thephebus FPT2 experiment. While the exercise concentrates mainly on code-to-code benchmarking, a comparison is also made with selected experimental results. The test scenario with the appropriate initial and boundary conditions was provided by the Institut de Radioprotection et de SÛreté Nucléaire. For the analyses, seven severe accident analysis codes were used: ASTEC, ATHLET-CD, MELCOR, ICARE2, ICARE/CATHARE, SCDAP/RELAP5, and RELAP/SCDAPSIM. The calculations focused on the following phenomena occurring in the FPT2 bundle: thermal behavior; hydrogen production, mainly due to cladding oxidation; severe degradation of irradiated fuel; and the release of fission products, control rod, and structure materials. Using the same postdefined boundary and initial conditions, the code-data differences are typically within 10% for most parameters, and not more than 25%. More importantly, the codes were able to capture the major features of the transient evolution. Given that Phebus FPT2 exhibited almost all of the major low-pressure severe accident phenomena except for core cooling by water injection and late-phase core melt behavior in the lower head, the results engender a degree of confidence in the code predictive capability for sequences similar toFPT2