Integration of Cool- and Warm-Season Grass Pasturing Systems into Cattle Finishing Programs

In a previous report (ISRF01-10), we reported on a study that demonstrated that fall-born steer calves pastured on bromegrass for either portions of or all of the grazing season and then finished in drylot, outperformed calves placed directly into the feedlot in terms of profit/head at harvest. Areas consisting of highly productive soils, interdispersed with highly erodible land, are well suited for this kind of production practice. Production systems of this nature are quite consistent with the concepts of sustainable agriculture. In an effort to capture more grazing potential, it was decided to incorporate warmseason grasses into the pasture program so that forage production would be enhanced during the hot summer months of July and August when cool-season grasses may become nearly dormant. Therefore, the objective of this multiyear study was to compare steer calves provided a combination of cool- and warm-season grass pastures with calves provided cool-season grass pastures only and followed by all calves being finished in drylot. Growth performance and carcass composition, were compared among treatments

Progress Report: Pasture Finishing of Beef Steers Using Contemporary Feedlot Protocols

Environmental regulations in Iowa can place animal population restrictions on a given site. This study explores the possibility of finishing cattle in a remote pasture location to handle an overflow of animals and yet remain in compliance with environmental mandates

Progress Report: Effects of Condensed Corn Distillers Solubles on Steer Performance and Carcass Composition

Condensed, corn, distillers solubles provides an effective energy supplement for finishing cattle across a number of finishing systems

Effectiveness of mindfulness-based stress reduction in mood, breast- and endocrine-related quality of life, and well-being in stage 0 to III breast cancer : a randomized, controlled trial

Purpose To assess the effectiveness of mindfulness-based stress reduction (MBSR) for mood, breast- and endocrine-specific quality of life, and well-being after hospital treatment in women with stage 0 to III breast cancer. Patients and Methods A randomized, wait-listed, controlled trial was carried out in 229 women after surgery, chemotherapy, and radiotherapy for breast cancer. Patients were randomly assigned to the 8-week MBSR program or standard care. Profile of Mood States (POMS; primary outcome), Functional Assessment of Cancer Therapy–Breast (FACT-B), Functional Assessment of Cancer Therapy–Endocrine Symptoms (FACT-ES) scales and the WHO five-item well-being questionnaire (WHO-5) evaluated mood, quality of life, and well-being at weeks 0, 8, and 12. For each outcome measure, a repeated-measures analysis of variance model, which incorporated week 0 measurements as a covariate, was used to compare treatment groups at 8 and 12 weeks. Results There were statistically significant improvements in outcome in the experimental group compared with control group at both 8 and 12 weeks (except as indicated) for POMS total mood disturbance (and its subscales of anxiety, depression [8 weeks only], anger [12 weeks only], vigor, fatigue, and confusion [8 weeks only]), FACT-B, FACT-ES, (and Functional Assessment of Cancer Therapy subscales of physical, social [8 weeks only], emotional, and functional well-being), and WHO-5. Conclusion MSBR improved mood, breast- and endocrine-related quality of life, and well-being more effectively than standard care in women with stage 0 to III breast cancer, and these results persisted at three months. To our knowledge, this study provided novel evidence that MBSR can help alleviate long-term emotional and physical adverse effects of medical treatments, including endocrine treatments. MBSR is recommended to support survivors of breast cancer

Robust, reproducible, industrialized, standard membrane feeding assay for assessing the transmission blocking activity of vaccines and drugs against Plasmodium falciparum.

BackgroundA vaccine that interrupts malaria transmission (VIMT) would be a valuable tool for malaria control and elimination. One VIMT approach is to identify sexual erythrocytic and mosquito stage antigens of the malaria parasite that induce immune responses targeted at disrupting parasite development in the mosquito. The standard Plasmodium falciparum membrane-feeding assay (SMFA) is used to assess transmission-blocking activity (TBA) of antibodies against candidate immunogens and of drugs targeting the mosquito stages. To develop its P. falciparum sporozoite (SPZ) products, Sanaria has industrialized the production of P. falciparum-infected Anopheles stephensi mosquitoes, incorporating quantitative analyses of oocyst and P. falciparum SPZ infections as part of the manufacturing process.MethodsThese capabilities were exploited to develop a robust, reliable, consistent SMFA that was used to assess 188 serum samples from animals immunized with the candidate vaccine immunogen, Pfs25, targeting P. falciparum mosquito stages. Seventy-four independent SMFAs were performed. Infection intensity (number of oocysts/mosquito) and infection prevalence (percentage of mosquitoes infected with oocysts) were compared between mosquitoes fed cultured gametocytes plus normal human O(+) serum (negative control), anti-Pfs25 polyclonal antisera (MRA39 or MRA38, at a final dilution in the blood meal of 1:54 as positive control), and test sera from animals immunized with Pfs25 (at a final dilution in the blood meal of 1:9).ResultsSMFA negative controls consistently yielded high infection intensity (mean = 46.1 oocysts/midgut, range of positives 3.7-135.6) and infection prevalence (mean = 94.2%, range 71.4-100.0) and in positive controls, infection intensity was reduced by 81.6% (anti-Pfs25 MRA39) and 97.0% (anti-Pfs25 MRA38), and infection prevalence was reduced by 12.9 and 63.5%, respectively. A range of TBAs was detected among the 188 test samples assayed in duplicate. Consistent administration of infectious gametocytes to mosquitoes within and between assays was achieved, and the TBA of anti-Pfs25 control antibodies was highly reproducible.ConclusionsThese results demonstrate a robust capacity to perform the SMFA in a medium-to-high throughput format, suitable for assessing large numbers of experimental samples of candidate antibodies or drugs

Integration of Cool- and Warm-Season Grass Pasturing Systems into Cattle Finishing Programs

Previously we reported on a study that demonstrated that fall-born steer calves pastured on bromegrass for either portions of or all of the grazing season and then finished in drylot, significantly outperformed calves placed directly into the feedlot in terms of profit/head at harvest time. Areas consisting of highly productive soils, interdispersed with highly erodable land, are well suited for this kind of production practice and in turn production systems of this nature are quite consistent with the concepts of sustainable agriculture. In an effort to capture more grazing potential, it was decided to incorporate warm-season grasses into the pasture program so that forage production would be enhanced during the hot summer months of July and August when cool-season grasses normally become nearly dormant. Therefore, the objective of this multi-year study is to compare steer calves provided a combination of cool- and warm-season grass pastures with calves provided cool-season grass pastures only and followed by all calves being finished in drylot

Progress Report: Effects of Condensed Corn Distillers Solubles on Steer Performance and Carcass Composition

This study looked at the use of condensed corn distillers solubles (CCDS) as a feed source for steers backgrounded on pasture as well as steers being fed in the feedlot. In addition, a treatment group was finished on pasture with CCDS as a part of their ration. The two feedlot rations were isocaloric and isonitrogenous. The pasture finishing steers were fed a ration based on the feedlot ration containing CCDS but were not fed any hay. The goal of this study was to establish the benefits, as well as the limitations, of feeding CCDS to feedlot and backgrounded steers

Effects of Condensed Corn Distillers Solubles on Steer Performance and Carcass Composition

Condensed corn distillers solubles (CCDS) have become a prevalent feed source in Iowa. In this study, we looked at the impact of CCDS in finishing steer rations and the influence CCDS had on steer performance as well as carcass composition. In the first year of the study, 112 steers were randomly sorted into four equal groups where each group contained four pens and 7 steers per pen. The second year of the study was a repeat of the first year however a fifth treatment was added in which 28 steers were placed on pasture and provided a finishing grain diet. The first of the four treatments consisted of the feedlot group (F), which was placed directly in to the feedlot and fed shelled corn, alfalfa hay, a protein, vitamin, and mineral supplement, and molasses. The second treatment was feedlot + CCDS (F+CCDS). This treatment group was placed directly into the feedlot and received shelled corn, alfalfa hay, a protein, vitamin and mineral supplement, and CCDS. The third treatment group was backgrounded on pasture for the duration of the summer (P), and then put into the feedlot where they received the same ration as the F group. The fourth treatment group was backgrounded on pasture for the duration of the summer, and while on pasture had access to free choice CCDS via a lick tank (P+CCDS). In the fall this group was placed into the feedlot and received the same ration as the F+CCDS group. In the second year, a fifth treatment was added, called the pasture finishing group (PF). The steers in this treatment received the same shelled corn, and protein, vitamin and mineral supplement, and CCDS as the cattle in the F+CCDS treatment, minus the alfalfa hay. Grass consumption for the PF cattle was estimated using the 2007 BRANDS program. Although the study is not yet completed, it appears as though CCDS can be implemented in feedlot rations successfully. The existing trends of the study would indicate that a feedlot ration containing CCDS will slightly increase steer ADG and improve F:G, without effecting QG

Mutational analysis of βCOP (Sec26p) identifies an appendage domain critical for function

<p>Abstract</p> <p>Background</p> <p>The appendage domain of the γCOP subunit of the COPI vesicle coat bears a striking structural resemblance to adaptin-family appendages despite limited primary sequence homology. Both the γCOP appendage domain and an equivalent region on βCOP contain the FxxxW motif; the conservation of this motif suggested the existence of a functional appendage domain in βCOP.</p> <p>Results</p> <p>Sequence comparisons in combination with structural prediction tools show that the fold of the COOH-terminus of Sec26p is strongly predicted to closely mimic that of adaptin-family appendages. Deletion of the appendage domain of Sec26p results in inviability in yeast, over-expression of the deletion construct is dominant negative and mutagenesis of this region identifies residues critical for function. The ArfGAP Glo3p was identified via suppression screening as a potential downstream modulator of Sec26p in a manner that is independent of the GAP activity of Glo3p but requires the presence of the COOH-terminal ISS motifs.</p> <p>Conclusion</p> <p>Together, these results indicate an essential function for the predicted βCOP appendage and suggest that both COPI appendages perform a biologically active regulatory role with a structure related to adaptin-family appendage domains.</p