The carboxyl-terminal two-thirds of the ADP/ATP carrier polypeptide contains sufficient information to direct translocation into mitochondria

The precursor of the mitochondrial inner membrane protein ADP/ATP carrier is cytoplasmically synthesized without an amino-terminal peptide extension. We constructed a truncated precursor lacking the 103 amino acids from the amino terminus (about a third of the protein). Import of the truncated precursor into mitochondria showed the import characteristics of the authentic precursor, including nucleoside triphosphate dependence, requirement for a protease-sensitive component on the mitochondrial surface, two-step specific binding to the outer membrane, and membrane potential-dependent translocation into the inner membrane. We conclude that, in contrast to all other mitochondrial precursor proteins studied so far, domains of the ADP/ATP carrier distant from the amino terminus can carry specific targeting information for transport into mitochondria

A monitoring device for pressurised-air-driven diaphragm-based artificial heart assist devices

A non-invasive device has been developed to monitor the diaphragm position and the blood flow in artificial heart assist devices equipped with a pressurised-air-driven diaphragm. Light scattering from the diaphragm is used as a mechanism for measuring. Information about the position of several points of the diaphragm can be obtained. The completely empty or filled situation can be detected and used for control purposes. Flow data can be extracted and bending characteristics of the diaphragm during operation can be studied

The space-time budget method in criminological research

This article reviews the Space-Time Budget method developed by Wikström and colleagues and particularly discusses its relevance for criminological research. The Space-Time Budget method is a data collection instrument aimed at recording, retrospectively, on an hour-by-hour basis, the whereabouts and activities of respondents during four days in the week before the interview. The method includes items about criminologically relevant events, such as offending and victimization. We demonstrate that the method can be very useful in criminology, because it enables the study of situational causes of crime and victimization, because it enables detailed measurement of theoretical concepts such as individual lifestyles and individual routine activities, and because it enables the study of adolescents’ whereabouts, which extends the traditional focus on residential neighborhoods. The present article provides the historical background of the method, explains how the method can be applied, presents validation results based on data from 843 secondary school students in the Netherlands and describes the methods’ strengths and weaknesses. Two case studies are summarized to illustrate the usefulness of the method in criminological research. The article concludes with some anticipated future developments and recommendations on further readings