Effects of reverberation on perceptual segregation of competing voices

Two experiments investigated the effect of reverberation on listeners’ ability to perceptually segregate two competing voices. Culling et al. [Speech Commun. 14, 71–96 (1994)] found that for competing synthetic vowels, masked identification thresholds were increased by reverberation only when combined with modulation of fundamental frequency (F0). The present investigation extended this finding to running speech. Speech reception thresholds (SRTs) were measured for a male voice against a single interfering female voice within a virtual room with controlled reverberation. The two voices were either (1) co-located in virtual space at 0° azimuth or (2) separately located at ±60° azimuth. In experiment 1, target and interfering voices were either normally intonated or resynthesized with a fixed F0. In anechoic conditions, SRTs were lower for normally intonated and for spatially separated sources, while, in reverberant conditions, the SRTs were all the same. In experiment 2, additional conditions employed inverted F0 contours. Inverted F0 contours yielded higher SRTs in all conditions, regardless of reverberation. The results suggest that reverberation can seriously impair listeners’ ability to exploit differences in F0 and spatial location between competing voices. The levels of reverberation employed had no effect on speech intelligibility in quiet. © 2003 Acoustical Society of America

Speech perception from monaural and binaural information

Two experiments explored the concept of the binaural spectrogram [Culling and Colburn, J. Acoust. Soc. Am. 107, 517-527 (2000)] and its relationship to monaurally derived information. In each experiment, speech was added to noise at an adverse signal-to-noise ratio in the NoS pi binaural configuration. The resulting monaural and binaural cues were analyzed within an array of spectro-temporal bins and then these cues were resynthesized by modulating the intensity and/or interaural correlation of freshly generated noise. Experiment 1 measured the intelligibility of the resynthesized stimuli and compared them with the original NoSo and NoS pi stimuli at a fixed signal-to-noise ratio. While NoS pi stimuli were approximately equal to 50% intelligible, each cue in isolation produced similar (very low) intelligibility to the NoSo condition. The resynthesized combination produced approximately equal to 25% intelligibility. Modulation of interaural correlation below 1.2 kHz and of amplitude above 1.2 kHz was not as effective as their combination across all frequencies. Experiment 2 measured three-point psychometric functions in which the signal-to-noise ratio of the original NoS pi stimulus was increased in 3-dB steps from the level used in experiment 1. Modulation of interaural correlation alone proved to have a flat psychometric function. The functions for NoS pi and for combined monaural and binaural cues appeared similar in slope, but shifted horizontally. The results indicate that for sentence materials, neither fluctuations in interaural correlation nor in monaural intensity are sufficient to support speech recognition at signal-to-noise ratios where 50% intelligibility is achieved in the NoS pi configuration; listeners appear to synergistically combine monaural and binaural information in this task, to some extent within the same frequency region

Serial position effects in recognition memory for odors: a reexamination

Seven experiments examined recognition memory for sequentially presented odors. Following Reed (2000), participants were presented with a sequence of odors and then required to identify an odor from the sequence in a test probe comprising 2 odors. The pattern of results obtained by Reed (2000, although statistically marginal) demonstrated enhanced recognition for odors presented at the start (primacy) and end (recency) of the sequence: a result that we failed to replicate in any of the experiments reported here. Experiments 1 and 3 were designed to replicate Reed (2000), employing five-item and seven-item sequences, respectively, and each demonstrated significant recency, with evidence of primacy in Experiment 3 only. Experiment 2 replicated Experiment 1, with reduced interstimulus intervals, and produced a null effect of serial position. The ease with which the odors could be verbally labeled was manipulated in Experiments 4 and 5. Nameable odors produced a null effect of serial position (Experiment 4), and hard-to-name odors produced a pronounced recency effect (Experiment 5); nevertheless, overall rates of recognition were remarkably similar for the two experiments at around 70%. Articulatory suppression reduced recognition accuracy (Experiment 6), but recency was again present in the absence of primacy. Odor recognition performance was immune to the effects of an interleaved odor (Experiment 7), and, again, both primacy and recency effects were absent. There was no evidence of olfactory fatigue: Recognition accuracy improved across trials (Experiment 1). It is argued that the results of the experiments reported here are generally consistent with that body of work employing hard-to-name visual stimuli, where recency is obtained in the absence of primacy when the retention interval is short

Errorless learning and spaced retrieval: How do these methods fare in healthy and clinical populations?

While errorless learning and spaced retrieval have both proved effective in helping many patients with acquired brain injury (ABI) and dementia learn novel information, it is not clear which of these principles we should apply to target treatment most effectively. To address this issue we conducted a systematic comparison of these principles in three experiments, comparing their effectiveness in healthy controls (N = 60), patients with ABI (N = 30), and patients with dementia (N = 15). Participants were asked to learn face-name associations, and the relative effectiveness of the principles over and above trial-and-error learning was investigated. The results were remarkably consistent across experiments: Both errorless learning and spaced retrieval produced greater accuracy in name recall than did trial-and-error learning, but recall under conditions of spaced retrieval was significantly better than that under errorless learning. We discuss the implications of these findings and suggest that spaced retrieval may be the stronger memory rehabilitation principle when it comes to learning face-name associations in people with mild to moderate memory impairment

Perceptual learning in humans: Roles of preexposure schedule, feedback, and discrimination assay

In three experiments, humans received preexposure to two compound flavours (AX and BX: saline–lemon and sucrose–lemon) that were presented either in an intermixed (e.g., AX, BX,... BX, AX,...) or a blocked (e.g., AX, AX,... BX, BX...) fashion. Subsequently, AXwas paired with an unpleasant bitter taste, and the discriminability of AX and BX was assessed using the accuracy of same/different judgements and by the extent to which any learned dislike of AX generalized to BX. When participants received feedback about the accuracy of their same/different judgements during preexposure those given intermixed preexposure were more accurate in making these judgements during the test than those given blocked preexposure (Experiments 1 and 2A), however, there was no evidence of any learned dislike in these experiments. In Experiment 2B, in which participants did not receive feedback about the accuracy of their judgements, there was no effect of the preexposure regime on same/different judgements, but there was a learned dislike of AX, and this generalized less to BX in participants given intermixed than in those given blocked preexposure. The beneficial effects of intermixed preexposure are consistent with results from other species (chicks and rats), but the differences created by the presence or absence of feedback place constraints on the analysis of these effects

Are two methods better than one? Evaluating the effectiveness of combining errorless learning with vanishing cues

A growing trend in memory rehabilitation is to combine learning principles to enhance treatment effects. While this makes intuitive sense, little is known about the added value of incorporating each method. A further complication is that some interventions, although primarily based on one learning principle, actually incorporate several, which again adds to the difficulty in differentiating the individual contribution of techniques. In this paper we report results of two experiments comparing the effectiveness of combining principles of errorless learning (EL) with vanishing cues (VC) relative to each in isolation. Healthy controls (N = 60), learning under standard and dual-task conditions, and patients with probable Alzheimer's disease (N = 22) took part in Experiments 1 and 2, respectively. In each experiment, participants were asked to learn greeble- or face-name associations, and memory was tested after interference or brief delay. For healthy controls, EL alone and EL + VC produced the best results, but there was no difference between these conditions. For the Alzheimer's patients, all treatment conditions (EL, VC, EL + VC) were significantly better than trial and error, and, in this case, we found that the combined method was significantly better than VC in isolation. Importantly, in both experiments there was little support for use of combined over individual learning principles

NOTUM from Apc-mutant cells biases clonal competition to initiate cancer

Funding Information: Acknowledgements We thank the Core Services and Advanced Technologies at the Cancer Research UK Beatson Institute (C596/A17196 and A31287), and particularly the Biological Services Unit, Histology Service and Molecular Technologies; members of the Sansom and Katajisto laboratories for discussions of the data and manuscript; and BRC Oxford for supplying patient material. O.J.S. and his laboratory members were supported by Cancer Research UK (A28223, A21139, A12481 and A17196). D.J.F. and M.C.H. were supported by the UK Medical Research Council (MR/R017247/1 and MR/J50032X/1, respectively). SpecifiCancer CRUK Grand Challenge (C7932/A29055) is funded by Cancer Research UK and the Mark Foundation for Cancer Research. P.K. and his laboratory members were supported by the Academy of Finland Centre of Excellence MetaStem (266869, 304591 and 320185), the ERC Starting Grant 677809, the Swedish Research Council 2018-03078, the Cancerfonden 190634, the Jane and Aatos Erkko Foundation and the Cancer Foundation Finland. N.P. was supported by the Finnish Cultural Foundation, the Biomedicum Helsinki Foundation, the Orion Research Foundation sr and The Paulo Foundation. P.V.F. was supported by Alzheimer’s Research UK and The Francis Crick Institute. The ARUK UCL Drug Discovery Institute receives its core funding from Alzheimer’s Research UK (520909). The Francis Crick Institute receives its core funding from Cancer Research UK (FC001002), the UK Medical Research Council (FC001002) and the Wellcome Trust (FC001002). Publisher Copyright: © 2021, The Author(s), under exclusive licence to Springer Nature Limited.The tumour suppressor APC is the most commonly mutated gene in colorectal cancer. Loss of Apc in intestinal stem cells drives the formation of adenomas in mice via increased WNT signalling(1), but reduced secretion of WNT ligands increases the ability of Apc-mutant intestinal stem cells to colonize a crypt (known as fixation)(2). Here we investigated how Apc-mutant cells gain a clonal advantage over wild-type counterparts to achieve fixation. We found that Apc-mutant cells are enriched for transcripts that encode several secreted WNT antagonists, with Notum being the most highly expressed. Conditioned medium from Apc-mutant cells suppressed the growth of wild-type organoids in a NOTUM-dependent manner. Furthermore, NOTUM-secreting Apc-mutant clones actively inhibited the proliferation of surrounding wild-type crypt cells and drove their differentiation, thereby outcompeting crypt cells from the niche. Genetic or pharmacological inhibition of NOTUM abrogated the ability of Apc-mutant cells to expand and form intestinal adenomas. We identify NOTUM as a key mediator during the early stages of mutation fixation that can be targeted to restore wild-type cell competitiveness and provide preventative strategies for people at a high risk of developing colorectal cancer.Peer reviewe