Growth curve of uninfected PA14 (dark grey curve) and of PA14 infected by Ab31 at an M.O.I. of 0.1 (light grey curve).

<p>Growth curve of uninfected PA14 (dark grey curve) and of PA14 infected by Ab31 at an M.O.I. of 0.1 (light grey curve).</p

Restriction enzyme analysis of the Ab31 genome.

<p>Ab31 phage DNA (2 μg) digested with <i>Eco</i>RI (1), <i>Hind</i>III (2), <i>Sma</i>I (3), <i>Ssp</i>I (4), <i>Not</i>I (5), <i>Cla</i>I (6), <i>Pvu</i>II (7), <i>Sal</i>I (8) and <i>Sph</i>I (9) were analyzed by electrophoresis on a 0.8% agarose gel. On the left the λDNA/<i>Hind</i>III and on the right 1 kbp ladder are reported and they were used as molecular weight markers (Mw).</p

Electron microscopy analysis of phage Ab31.

<p>Scale bar represents 100</p

Schematic representation of the Ab31 insertion region.

<p>A 64<i>P. aeruginosa</i> Tr60 genome. The portion of the shared region that overlaps with the phage integrase encoding gene is underlined.</p

List of coding sequences (CDS) and their position in the 234 kbp deleted region.

<p>List of coding sequences (CDS) and their position in the 234 kbp deleted region.</p

List of the strains used and susceptibility to Ab31.

a<p>UN, unknown.</p>b<p>5 μL of Ab31 stock suspension (≈ 10⁸PFU/ml) were spotted on <i>P. aeruginosa</i> lawns. C+++, complete clearing; trace, a few individual plaques; 0, a turbid spot where the pipette tip touched the agar.</p

Annotation of the Ab31 genome.

<p>The Ab31 hypothetical terminal ends are at the 1 position. The morphogenesis module includes proteins similar to those encoded by phage AF (red), whereas the replication, recombination and lysis modules consist of genes similar to those of phage PAJU2 (blue). Genes encoding hypothetical proteins which have homologies with other phages are shown in purple color. Genes encoding hypothetical proteins of unknown function are shown in green color.</p

PCR investigation of the 234(A) and Flank234 primers (B).

<p>Tr60 (1), Tr60-10A (2), Tr60-100A (3), Tr60-100B (4), PA14-P1 (5), PA14 (6), Negative control (7). The experimental conditions are those of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093777#pone-0093777-g005" target="_blank">Fig.5</a>.</p

PCR investigation of the 11 kbp (A) and 22 kbp (B) deletions observed in Tr60.

<p>C9-12 (1), C5-17 (2), C7-11 (3), C8-12 (4), PAO1 (5), PA14 (6), Tr162 (7), Tr60 (8), Tr60-10A (9), Tr60-100A (10). Samples were run in a 2% agarose gel for 45 min at 135 V. Mw, 100 bp ladder molecular weight markers.</p

List of primers used for PCR amplification.

<p>List of primers used for PCR amplification.</p