(A) 12 h after CLP or sham laparotomy, the number of peritoneal granulocytes in WT or TLR9 mice (three to five mice per group) was determined by flow cytometry

(B) WT or TLR9 mice received 500 μg anti-GR1 antibody or isotype control 24 and 2 h before CLP, and the survival rates were monitored for 6 d after CLP (15 mice per group, with data pooled from three experiments, each of which had similar statistical significance). (C) 2 × 10 CD45.1 splenic granulocytes were injected i.v. into WT or TLR9 (CD45.2) recipients (three to five mice per group). 12 h after transfer, the mice underwent CLP or sham laparotomy, and 12 h later the number of CD45.1 granulocytes were enumerated from the peritoneal cells by flow cytometry. (D) 12 h after CLP or sham laparotomy, the number of granulocytes (Ly6G) was determined by flow cytometry in the peritoneal fluid of WT mice pretreated with 10 Flt3L-expanded WT or TLR9 DCs (three to five mice per group). Treatment with anti-GR1 antibody achieved >97% depletion of granulocytes at the time of and 24 h after CLP (not depicted). Data shown are means of values ± SEM obtained from individual mice and are representative of at least two independent experiments. *, P < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Toll-like receptor 9 inhibition reduces mortality in polymicrobial sepsis"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1277-1283.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413026.</p><p></p

(A) Immediately before CLP, WT mice received 100 μg iCpG or control oligodeoxynucleotide (ODN), and survival rates were monitored for 6 d

(B) 12 and 18 h after CLP, WT mice received 100 μg iCpG or control ODN, and survival rates were monitored for 6 d. Survival curves of mice treated with iCpG at 3 and 6 h were similar to the one shown for the 12-h iCpG group (not depicted). Data shown include 15 mice per group, pooled from three experiments, each of which had similar statistical significance.<p><b>Copyright information:</b></p><p>Taken from "Toll-like receptor 9 inhibition reduces mortality in polymicrobial sepsis"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1277-1283.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413026.</p><p></p

(A) The survival rates of WT B6 and TLR9 mice were monitored for 6 d after CLP (15 mice per group, with data pooled from three experiments, each of which had similar statistical significance)

Dilutions of blood (B) or peritoneal lavage fluid (C) obtained from TLR9 or WT mice 12 h after CLP were cultured on BHI agar plates, and the number of bacterial colonies was counted (five mice per group). The colony count indicated is of 1 ml of blood and 1 ml of peritoneal fluid. (D) Serum cytokine levels were determined 6 and 12 h after CLP by using a cytometric bead array (five mice per group). Data shown are means of values ± SEM obtained from individual mice and are representative of at least two independent experiments. *, P < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Toll-like receptor 9 inhibition reduces mortality in polymicrobial sepsis"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1277-1283.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413026.</p><p></p

(A) 12 h after CLP or sham laparotomy, the number of conventional DCs (CD11cMHCII) were determined by flow cytometry in the spleen and peritoneal fluid of WT or TLR9 mice (three to five mice per group)

(B) The survival rates of WT mice receiving WT or TLR9 DCs were monitored for 6 d after CLP (23 mice per group, with data pooled from two experiments, each of which had similar statistical significance). (C) 10 Flt3L-expanded WT or TLR9 DCs (CD45.2) were injected i.v. into CD45.1 WT recipients (three to five mice per group). 12 h after transfer, the mice underwent CLP or sham laparotomy, and 12 h later the number of CD45.2 DCs were enumerated from the peritoneal cells by flow cytometry. (D) Dilutions of blood or peritoneal lavage fluid obtained from WT mice pretreated with WT or TLR9 DCs 12 h after CLP were cultured on BHI agar plates, and the number of bacterial colonies was counted (five mice per group). (E) Serum cytokine levels were determined 12 h after CLP by cytometric bead array (five mice per group). Data shown are means of values ± SEM obtained from individual mice and are representative of at least two independent experiments. *, P < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Toll-like receptor 9 inhibition reduces mortality in polymicrobial sepsis"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1277-1283.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413026.</p><p></p

12 h after CLP or sham laparotomy, the numbers of viable splenocytes (A) and infiltrating peritoneal lymphocytes (B) were counted on a hemocytometer (BrightLine; Hausser Scientific) after Trypan blue staining (three to five mice per group)

Peritoneal cells are expressed per milliliter of peritoneal fluid. (C) Peritoneal cells pooled from three to five mice 12 h after CLP were cultured without restimulation for 24 h, and the supernatant cytokine levels were determined with a cytometric bead array. Peritoneal cells from mice that underwent sham laparotomy secreted a minimal amount of cytokines (not depicted). Data shown are means of values ± SEM obtained from individual mice (except for cells pooled in C) and are representative of at least two independent experiments. *, P < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Toll-like receptor 9 inhibition reduces mortality in polymicrobial sepsis"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1277-1283.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413026.</p><p></p