1,942 research outputs found
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Regulation of axon repulsion by MAX-1 SUMOylation and AP-3.
During neural development, growing axons express specific surface receptors in response to various environmental guidance cues. These axon guidance receptors are regulated through intracellular trafficking and degradation to enable navigating axons to reach their targets. In Caenorhabditis elegans, the UNC-5 receptor is necessary for dorsal migration of developing motor axons. We previously found that MAX-1 is required for UNC-5-mediated axon repulsion, but its mechanism of action remained unclear. Here, we demonstrate that UNC-5-mediated axon repulsion in C. elegans motor axons requires both max-1 SUMOylation and the AP-3 complex β subunit gene, apb-3 Genetic interaction studies show that max-1 is SUMOylated by gei-17/PIAS1 and acts upstream of apb-3 Biochemical analysis suggests that constitutive interaction of MAX-1 and UNC-5 receptor is weakened by MAX-1 SUMOylation and by the presence of APB-3, a competitive interactor with UNC-5. Overexpression of APB-3 reroutes the trafficking of UNC-5 receptor into the lysosome for protein degradation. In vivo fluorescence recovery after photobleaching experiments shows that MAX-1 SUMOylation and APB-3 are required for proper trafficking of UNC-5 receptor in the axon. Our results demonstrate that SUMOylation of MAX-1 plays an important role in regulating AP-3-mediated trafficking and degradation of UNC-5 receptors during axon guidance
A STUDY OF THE INFLUENCES OF KNOWLEDGE BOUNDARY SPANNING ON PROJECT PERFORMANCE IN INFORMATION SYSTEM DEVELOPMENT PROJECTS
Information system development (ISD) is a knowledge intensive process, and a socialize cross-disciplines collaboration that brings up innovations and creates a competitive advantage for the organization. However, different layers of knowledge boundaries (syntactic, semantic, pragmatic) arise with the knowledge diversity of the ISD project and further lowers the project performance and product quality. To solve the problems, we will follow a construct development methodology to empirically identify the critical knowledge boundary spanning (KBS) processes, roles and objects for different layers of knowledge boundary, examine their influences to the effectiveness of corresponding KBS, and further assess the direct and moderating relationships from KBS effectiveness to project performance and product quality through a questionnaire survey. For academic applications, we not only split up the layers of KBS effectiveness and examine their direct and moderating effects to ISD performance but also offer categorized KBS activities under a formal construct development methodology for future studies. For practical implications, we offer a model for ISD team members to refer to for solving their knowledge boundary issues and increase their project performance and product quality
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Intensification of Premonsoon Tropical Cyclones in the Bay of Bengal and Its Impacts on Myanmar
We analyze multiple global reanalysis and precipitation datasets in order to explain the dynamic mechanisms that lead to an observed intensification of the monsoon trough and associated tropical cyclone (TC) activity over the Bay of Bengal (BOB) during the premonsoon month of May. We find that post-1979 increases in both premonsoon precipitation and TC intensity are a result of enhanced large-scale monsoon circulation, characterized by lower-level cyclonic and upper-level anticyclonic anomalies. Such circulation anomalies are manifest of the tropospheric expansion that is caused by regional warming. The deepened monsoon trough in the BOB not only affects TC frequency and timing, but also acts to direct more cyclones towards Myanmar. We propose that increasing sea surface temperature in the BOB has contributed to an increase in cyclone intensity. Our analyses of the Community Earth System Model single-forcing experiments suggest that tropospheric warming and a deepening of the monsoon trough can be explained by two discreet anthropogenic causes--an increase in absorption due to aerosol loading and an increase in the land-ocean thermal contrast that results from increased greenhouse gases. The ensuing circulation changes provide favorable conditions for TCs to grow and to track eastward towards Myanmar
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