Separate loci underlie resistance to root infection and leaf scorch during soybean sudden death syndrome

Soybean [Glycine max (L.) Merr.] cultivars show differences in their resistance to both the leaf scorch and root rot of sudden death syndrome (SDS). The syndrome is caused by root colonization by Fusarium virguliforme (ex. F. solani f. sp. glycines). Root susceptibility combined with reduced leaf scorch resistance has been associated with resistance to Heterodera glycines HG Type 1.3.6.7 (race 14) of the soybean cyst nematode (SCN). In contrast, the rhg1 locus underlying resistance to Hg Type 0 was found clustered with three loci for resistance to SDS leaf scorch and one for root infection. The aims of this study were to compare the inheritance of resistance to leaf scorch and root infection in a population that segregated for resistance to SCN and to identify the underlying quantitative trait loci (QTL). “Hartwig”, a cultivar partially resistant to SDS leaf scorch, F. virguliforme root infection and SCN HG Type 1.3.6.7 was crossed with the partially susceptible cultivar “Flyer”. Ninety-two F5-derived recombinant inbred lines and 144 markers were used for map development. Four QTL found in earlier studies were confirmed. One contributed resistance to leaf scorch on linkage group (LG) C2 (Satt277; P = 0.004, R 2 = 15%). Two on LG G underlay root infection at R8 (Satt038; P = 0.0001 R 2 = 28.1%; Satt115; P = 0.003, R 2 = 12.9%). The marker Satt038 was linked to rhg1 underlying resistance to SCN Hg Type 0. The fourth QTL was on LG D2 underlying resistance to root infection at R6 (Satt574; P = 0.001, R 2 = 10%). That QTL was in an interval previously associated with resistance to both SDS leaf scorch and SCN Hg Type 1.3.6.7. The QTL showed repulsion linkage with resistance to SCN that may explain the relative susceptibility to SDS of some SCN resistant cultivars. One additional QTL was discovered on LG G underlying resistance to SDS leaf scorch measured by disease index (Satt130; P = 0.003, R 2 = 13%). The loci and markers will provide tagged alleles with which to improve the breeding of cultivars combining resistances to SDS leaf scorch, root infection and SCN HG Type 1.3.6.7

Transportation Package Seal Performance in Beyond Design Basis Thermal Exposures -12472

ABSTRACT The Nuclear Regulatory Commission (NRC) technical report, NUREG/CR-6886, "Spent Fuel Transportation Package Response to the Baltimore Tunnel Fire Scenario," describes, in detail, an evaluation of the potential for a theoretical release of radioactive material from three different spent nuclear fuel (SNF) transportation packages, had they been exposed to the Baltimore tunnel fire that occurred in July of 2001. This evaluation determined the temperatures of various components of the packages, including the seals, using temperatures resulting from models of the Baltimore tunnel fire (as boundary conditions) and finite element models of the SNF packages. For two of the packages evaluated, the analyses indicated that the seals used would have exceeded their continuous-use rated service temperatures, meaning the release of radioactive material could not be ruled out with available information; However, for both of the packages evaluated, the analysis determined, by a bounding calculation, that the maximum potential release was well below the regulatory requirements for releases from a SNF package during the hypothetical accident condition (HAC) sequence of events in 10CFR Part 71. The NRC is investigating the performance of seals in SNF transportation packages exposed to fires that could exceed the HAC fire described in 10CFR Part 71, such as the Baltimore Tunnel Fire that occurred in 2001. The performance of package seals is important for determining the potential release of radioactive material from a package during a beyond-design-basis accident. The seals have lower temperature limits than other package components and are the containment barrier between the environment and the cask contents. The NRC Office of Nuclear Regulatory Research contracted the National Institute of Standards and Technology (NIST) to conduct small-scale thermal testing to obtain experimental data of the performance of seals during extreme temperature exposures. The experimental testing consisted of several small-scale pressure vessels fabricated with a modified ASME flange design and tested metallic and elastomeric seals, similar to those that might be used on an actual SNF transportation package. The vessels were heated in an electrical oven to temperatures as high as 800°C (1472°F), exceeding the rated temperatures of the seals in question. This paper will provide a summary of the testing conducted and present test results and conclusions

The Relations Among Upstander, Bystander, Reinforcer, and Perpetrator Bullying Behaviors in Middle School Students

Bullying is a persistent and common problem in schools nationwide. The experience of bullying can affect the social, psychological, academic, and emotional development of children. Youth can be involved in bullying in various ways, including as a victim, perpetrator, witness, or bystander. Upstanders play a significant role in the prevention of bullying, and the presence of upstanders in the student body is related to positive school climate. Minimal research on upstander behavior currently exists in the literature. The purpose of this study was to evaluate specific predictors of witness roles utilizing demographic and other variables. The data from 3438 student surveys analyzed in the present study were collected from a prior study on school climate across 35 schools in Southern Illinois. Results indicated that gender, grade, and minority status were predictors of upstander behavior. However, no demographic variables predicted either bystander or reinforcer behaviors As expected, indirect upstander behaviors occurring after an incident were reported less frequently than behaviors occurring directly with the victim, or in the moment. Upstander behaviors occurring in the moment were significantly and positively correlated with other upstander behaviors, and negatively correlated with perpetrator behaviors, whereas reinforcer behaviors were significantly and positively correlated. The results of this study and implications for future interventions are discussed

Spent Nuclear Fuel Transportation Package Seals in Beyond Design Basis Temperature Excursions

The US Nuclear Regulatory Commission (NRC) is studying the performance of seals in spent nuclear fuel (SNF) transportation packages exposed to fires that could exceed the hypothetical accident condition fire described in Title 10 of the Code of Federal Regulations, Part 71, such as the Baltimore Tunnel Fire that occurred in 2001, or the MacArthur Maze fire that occurred in 2007. The performance of package seals is important for determining the potential for release of radioactive material from a package during a beyond-design-basis accident. Seals generally have lower temperature limits than other package components and are the containment barrier between the environment and the radioactive package contents. The NRC Office of Nuclear Regulatory Research contracted the National Institute of Standards and Technology to conduct small-scale thermal testing to obtain experimental data of the performance of seals during extreme temperature exposures. The experimental testing consisted of several small-scale pressure vessels fabricated with a modified ASME flange design and tested metallic and polymeric seals, similar to those that might be used on an actual SNF transportation package. The vessels were heated in an electrical oven to temperatures as high as 800°C (1472°F), exceeding the rated temperatures of the seals in question. This paper will provide a summary of the testing conducted and present test results and conclusions.</jats:p