Effects of Selenium compounds eye drops on the cornea of a dry eye rat model.

<p>Fluorescein score ratios of cornea in dry eye rat treated with selenium compounds (A, C–F), apolactoferrin (B), or PBS (A–F) or are shown. The score of PBS treatment was defined as 1. Results are expressed as mean ± S.D (n = 10). Dunnett’s test was used to determine the significance of differences. * and *** indicate a significant difference from the result in PBS treatment, P<0.05 and <0.005, respectively (A).</p

Effect of Se-lactoferrin eye drops on expression of RNAs in the corneas.

<p>The vertical axis shows the expression ratio of RNAs. Fold change shows the expression ratio of mRNAs in the NT, PBS, and 0.1%Se-loactoferrin groups. The expression level in the NT group was defined as 1. Results are expressed as the mean ± S.D. (n = 4).</p

Effect of selenium compounds in CEPI cells.

<p>A: Effect of selenium compounds on cellular viability of CEPI cells. The horizontal axis shows the selenium concentration of each selenium compound. B: Recovery of GPx activity by the addition of selenium compounds. The vertical axis shows the GPx activity ratio. Results are expressed as mean ± S.D.</p

Effect of Se-lactoferrin eye drops on production of 8-OHdG in corneas.

<p>A, B: Immunohistochemical staining of corneas of normal (A) and dry eye (B) rats using an anti-8-OHdG antibody. C,D: Immunohistochemical staining of corneas of dry eye rats treated with PBS (C) or 0.1% Se-lactoferrin (D) using anti-8-OHdG antibody. Scale bar shows 20 µm. Original magnification: x20. E: Relative ratio of 8-OHdG in corneas of normal, dry eye, dry eye treated with PBS or dry eye treated with 0.1% Se-lactoferrin rat. The vertical axis shows 1/brightness ratio. The brightness ratio in the normal group was defined as 1. Results are expressed as the mean ± S.D. (n = 4). Dunnett’s test was used to determine the significance of differences. *** indicate a significant difference from the results in normal or PBS treatment group, P<0.005.</p

Comparison of capacity of apolactoferrin and lactoferrin for selenium binding

<p>.The selenium content in apolactoferrin and normal lactoferrin is shown. The vertical axis shows selenium content (mg) in 100g of apolactoferrin or normal lactoferrin. Results are expressed as mean ± S.D (n = 6).</p