PAGE analysis of BER activities of AlkA, hMPG and HeLa CFE1s for Oxa and Oxa–Sp

<p><b>Copyright information:</b></p><p>Taken from "Repair activity of base and nucleotide excision repair enzymes for guanine lesions induced by nitrosative stress"</p><p>Nucleic Acids Research 2005;33(7):2181-2191.</p><p>Published online 14 Apr 2005</p><p>PMCID:PMC1079971.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> () 25OXA, 25OXA–SP and 34MG (all 2 nM, and ) were incubated with the indicated amounts of AlkA and hMPG at 37°C for 30 min. After incubation, the reaction mixture was treated with 0.1 M NaOH to cleave AP sites, and products were separated by 16% denaturing PAGE. The nicked products due to β-elimination (upper bands) and β,δ-elimination (lower bands) are indicated by open brackets. () 25OXA and 25HX (both 2 nM, and ) were incubated in hMPG buffer with the indicated amounts of HeLa CFE1s at 37°C for 1 h. Products were analyzed as described above