Reply to "Comment on 'Isotope effect in multi-band and multi-channel attractive systems and inverse isotope effect in iron-based superconductors'"

The Comment insists on the following: in our model it is assumed that the effective interactions have specific energy ranges within the single band with a cutoff at \omega_1 for the phononic part and a range from \omega_1 to \omega_2 in the AF channel. Our reply is that we assume that V_i(k,k')\neq 0 if |\xi_k|<\omega_i and |\xi_{k'}|<\omega_i, and otherwise V_i(k,k')= 0 (i=1,2), as stated in our paper. This is the model of BCS type with two attractive interactions, and this assumption is the characteristic of the BCS approximation. The claim "the integration limits have been modified such that the AF channel mediated pairing sets in where the ph-channel pairing terminates and is limited at an energy given by \omega_j=\omega_{AF}" in the Comment is wrong. We describe the model and the method to solve the gap equation in more detail

Toward the Cross-Institutional Data Integration From Shibboleth Federated LMS

Through this study, we aim to examine a method for data integration in shared Learning Management System (LMS) in authentication federation. We proposed a method of transmitting ePTID and learning data with user’s consent as a method for data integration across institutions. The method is compared with the other existing methods to realize the shared LMS. We discuss the suitable method for next version of GakuNinMoodle and conclude that our requirements are not fully satisfied by a single method

Modified Method of Kluver-Barrera's Staining with Microwave Irradiation

クリューバー･バレラ(KB)染色は中枢神経系の白質における重要成分である髄鞘を選択的に染色する方法として日常的に広く使われている｡しかし現在の所,ルクソール･ファスト青(LFB)染色後の0.05%炭酸リチウム及び70%アルコールによる分別時間が確定されていないので,分別の良否を経験的にしか判断せざるをえないという欠点がある｡そこで,本研究において,マイクロウェーブ照射をLFB染色に応用することで,その後の分別について検討した結果,従来の0.05%炭酸リチウムでは良好な結果が得られなかったが分別液として,0.05%炭酸水素ナトリウムおよび70%アルコールを用いることで分別時間の確定が可能となった。Kluver-Barrera's (KB) staining is the well-known and widely used method for staining the Schwann's sheath of the central nervous systems. On the contrary, the optimum time is not established yet, which incubated with 0.05% lithium carbonate and 70% alcohol after luxol fast blue staining. The modified method was studied that was stained with the microwave irradiation and used 0.05% sodium bicarbonate in stead of 0.05% lithium carbonate. This method was simple and useful to differentiate Schwann's sheath from other components

Water balance in healthy and handicapped adults

The body’s water balance is changed by food and beverage intake, metabolism, and excretion. In this study, we performed a cross-sectional study that investigated the changes of water intake and water output in healthy Japanese young and elderly people and handicapped adults. Water balance was assessed by water intake from foods and beverages, metabolic water production, non-renal water losses (NRWL), and urine volume. Most of the parameters did not change with aging in healthy adults. Estimated total water intake (ml / kg / day) increased with aging. In the healthy men, healthy women, and handicapped adults, daily water intake (median [interquartile range]) accounted for 49.4 (41.4-59.9) ml / kg, 42.9 (38.7-51.8) ml / kg, and 50.9 (43.8-74.0) ml / kg, respectively. Water loss from the kidney accounted for 19.2 (16.2-29.2) ml / kg, 22.0 (16.2-26.6) ml / kg, and 27.5 (22.7- 47.2) ml / kg, respectively. NRWL accounted for 26.6 (18.5-35.2) ml / kg, 22.4 (16.2-28.8) ml / kg, and 23.5 (19.8-28.5) ml / kg, respectively. Our findings suggest that a daily total water intake of more than 50-55 ml / kg is required to prevent dehydration in healthy and handicapped adults

AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis

Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4;4 protein exhibit membrane potential- and Cl-dependent ascorbate uptake. The AtPHT4;4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4;4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4;4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress

Structural basis for high selectivity of a rice silicon channel Lsi1

Silicon (Si), the most abundant mineral element in the earth’s crust, is taken up by plant roots in the form of silicic acid through Low silicon rice 1 (Lsi1). Lsi1 belongs to the Nodulin 26-like intrinsic protein subfamily in aquaporin and shows high selectivity for silicic acid. To uncover the structural basis for this high selectivity, here we show the crystal structure of the rice Lsi1 at a resolution of 1.8 Å. The structure reveals transmembrane helical orientations different from other aquaporins, characterized by a unique, widely opened, and hydrophilic selectivity filter (SF) composed of five residues. Our structural, functional, and theoretical investigations provide a solid structural basis for the Si uptake mechanism in plants, which will contribute to secure and sustainable rice production by manipulating Lsi1 selectivity for different metalloids