Status Report of Neutral Kaon photo-production study using Neutral Kaon Spectrometer 2 (NKS2) at LNS-Tohoku(I. Nuclear Physics)

The approach described in this paper uses an array of Field Programmable Gate Array (FPGA) devices to implement a fault tolerant hardware system that can be compared to the running of fault tolerant software on a traditional processor. Fault tolerance is achieved is achieved by using FPGA with on the fly partial programmability feature. Major considerations while mapping to the FPGA includes the size of the area to be mapped and communication issues related to their communication. Area size selection is compared to the page size selection in Operating System Design. Communication issues between modules are compared to the software engineering paradigms dealing with module coupling, fan-in, fan-out and cohesiveness. Finally, the overhead associated with the downloading of the reconfiguration files is discussed

Direct determination of Burgers vector sense and magnitude of elementary dislocations by synchrotron white x-ray topography

The x-ray topography by using highly coherent beam obtained at third-generation synchrotron facilities can provide higher spatial resolution and higher lattice-distortion sensitivity than those by former-generation facilities. Here, we report the direct determination of the Burgers vector senses and magnitudes of elementary dislocations in a high-quality silicon carbide single crystal using white x-ray section topography with a long sample-to-film distance. Our data strongly indicate that there are very weak but extraordinarily long-range elastic interactions between elementary screw dislocations. Those interactions govern dislocation-propagation behavior and the distribution of dislocations. Moreover, we found that white x-ray projection topography with a long sample-to-film distance can also be a powerful tool to effectively examine the detailed structure of elementary dislocations in single crystals

Improved cytosolic delivery of macromolecules through dimerization of attenuated lytic peptides

Intracellular delivery of biomacromolecules is a challenging research field in chemical biology and drug delivery. We previously reported a peptide named L17E, which successfully delivered functional proteins, including antibodies, into cells. However, relatively high concentrations of L17E and proteins are needed. In this study, we prepared dimers of L17E and its analog L17E/Q21E. Dimerization of L17E increased cytotoxicity leading to reduced intracellular delivery compared with L17E. On the other hand, the dimers of the L17E analog, L17E/Q21E, especially when tethered at the N-termini, yielded a comparable level of intracellular delivery with L17E at decreased amounts of delivery peptides and cargoes

Direct determination of Burgers vector sense and magnitude of elementary dislocations by synchrotron white x-ray topography

The x-ray topography by using highly coherent beam obtained at third-generation synchrotron facilities can provide higher spatial resolution and higher lattice-distortion sensitivity than those by former-generation facilities. Here, we report the direct determination of the Burgers vector senses and magnitudes of elementary dislocations in a high-quality silicon carbide single crystal using white x-ray section topography with a long sample-to-film distance. Our data strongly indicate that there are very weak but extraordinarily long-range elastic interactions between elementary screw dislocations. Those interactions govern dislocation-propagation behavior and the distribution of dislocations. Moreover, we found that white x-ray projection topography with a long sample-to-film distance can also be a powerful tool to effectively examine the detailed structure of elementary dislocations in single crystals

An Artificial Amphiphilic Peptide Promotes Endocytic Uptake by Inducing Membrane Curvature

International audienc

Liquid droplet formation and facile cytosolic translocation of IgG in the presence of attenuated cationic amphiphilic lytic peptides

抗体を液滴に濃縮し細胞内へ高速輸送 --クモ毒改良ペプチドと抗体による液－液相分離の誘起と抗体の細胞内輸送--. 京都大学プレスリリース. 2021-08-06.Fc region binding peptide conjugated with attenuated cationic amphiphilic lytic peptide L17E trimer [FcB(L17E)₃] was designed for immunoglobulin G (IgG) delivery into cells. Particle-like liquid droplets were generated by mixing Alexa Fluor 488 labeled IgG (Alexa488-IgG) with FcB(L17E)₃. Droplet contact with the cellular membrane led to spontaneous influx and distribution of Alexa488-IgG throughout cells in serum containing medium. Involvement of cellular machinery accompanied by actin polymerization and membrane ruffling was suggested for the translocation. Alexa488-IgG negative charges were crucial in liquid droplet formation with positively charged FcB(L17E)₃. Binding of IgG to FcB(L17E)₃ may not be necessary. Successful intracellular delivery of Alexa Fluor 594-labeled anti-nuclear pore complex antibody and anti-mCherry-nanobody tagged with supernegatively charged green fluorescence protein allowed binding to cellular targets in the presence of FcB(L17E)₃

Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides

抗体を液滴に濃縮し細胞内へ高速輸送 --クモ毒改良ペプチドと抗体による液－液相分離の誘起と抗体の細胞内輸送--. 京都大学プレスリリース. 2021-08-06.Fc region binding peptide conjugated with attenuated cationic amphiphilic lytic peptide L17E trimer [FcB(L17E)₃] was designed for immunoglobulin G (IgG) delivery into cells. Particle-like liquid droplets were generated by mixing Alexa Fluor 488 labeled IgG (Alexa488-IgG) with FcB(L17E)₃. Droplet contact with the cellular membrane led to spontaneous influx and distribution of Alexa488-IgG throughout cells in serum containing medium. Involvement of cellular machinery accompanied by actin polymerization and membrane ruffling was suggested for the translocation. Alexa488-IgG negative charges were crucial in liquid droplet formation with positively charged FcB(L17E)₃. Binding of IgG to FcB(L17E)₃ may not be necessary. Successful intracellular delivery of Alexa Fluor 594-labeled anti-nuclear pore complex antibody and anti-mCherry-nanobody tagged with supernegatively charged green fluorescence protein allowed binding to cellular targets in the presence of FcB(L17E)₃