Legislative Documents

Also, variously referred to as: House bills; House documents; House legislative documents; legislative documents; General Court documents

Legislative Documents

Also, variously referred to as: House bills; House documents; House legislative documents; legislative documents; General Court documents

Additional file 2: of A central-acting connexin inhibitor, INI-0602, prevents high-fat diet-induced feeding pattern disturbances and obesity in mice

Figure S2. The SFAs in HFDs disrupted feeding patterns by increasing light cycle intake. (a–c) Mice were fed one of two diets high in saturated fatty acids (SFAs); the HFD60 contained higher amounts of C16:0 and C18:0 (n = 6, black circles with solid line) than the HFD32 (n = 8, white squares with dashed line). After acclimation to FDAMS with NC, mice were fed the HFDs for 5 days. White and black bars on the X-axis correspond to the light and dark cycles, respectively. (a-b) Hourly caloric intake (1 kcal = 4.186 kJ) before (Pre) and after the diet switch from NC to (a) HFD60 or (b) HFD32. (c) The light cycle intake expressed as a percentage of the 24-h intake. Black circles with solid line: the HFD60 group; white squares with dashed line: the HFD32 group. Data are the means ± s.e.m. Statistical significance was determined with the Student’s paired t-test for comparisons to pre-diet values for each group, in c; *P < 0.05. Abbreviations: NC, normal chow; HFD, high-fat diet; FDAMS, feeding drinking, and activity monitoring system. (TIF 226 kb

Enhanced proliferation of pancreatic duct epithelial cells and development of polycystic pancreas in TG mice.

<p>(A) Immunohistochemistry with anti-Ki67 antibody (red) along with Lectin-DBA (green) was conducted in pancreatic sections from 2 month old TG and control mice. Representative results are shown. (B) Photographs of the pancreas in 12 month old TG and control mice. Representative pictures are shown. (C) Hematoxylin and eosin (HE) staining (left panel indicates ×40 magnification and right ×400) in pancreatic sections from 12 month old TG mice. Representative images are shown. (D) Immunohistochemistry with anti-Ki67 antibody (red) along with Lectin-DBA (green) was conducted in pancreatic sections from 12 month old TG mice. Representative images of pancreatic cysts are shown. Arrow indicates Ki67 positive cells.</p

Islet vascularity and VEGF-A expression in HFHSD fed mice and <i>db/db</i> mice.

<p>(A) Immunohistochemistry with anti-PECAM1 or anti-VEGF-A antibodies in pancreatic sections from HFHSD fed mice, <i>db/db</i>, and control mice. Six male mice from each genotype and six sections per mouse were analyzed. Representative images are shown. (B) Islets were isolated from HFHSD fed mice, <i>db/db</i>, and control mice and used for analyses by real-time RT-PCR to quantify PECAM1 and VEGF-A mRNA levels. Six male mice were analyzed for each genotype. The results were normalized using GAPDH. Data represent the mean ± SEM fold-increase relative to control mice. An asterisk indicates <i>P</i><0.05 by ANOVA.</p

Enhanced VEGF-A expression in β cells but not α cells in TG mice.

<p>(<b>A</b>) Double immunostaining of VEGF-A with insulin or glucagon in pancreatic sections from TG mice and control mice. Enhanced VEGF-A staining (red) in TG mice is completely merged with insulin staining (green to yellow) but not with glucagon staining (green). (B) Quantitative analysis of VEGF-A staining levels in the islets of TG and control mice. We measured the staining levels of VEGF-A per unit area by using NIS-Elements (Nikon) and Image-J (NIH). Six mice were analyzed for each genotype and representative images are shown.</p

Hyperglycemia and impaired glucose tolerance in TG mice.

<p>(A) Blood glucose levels (mg/dl) in fasted and ad-lib fed TG mice and their control littermates at 8 weeks of age. (B) Plasma insulin levels (ng/ml) before and 15 min after glucose (1.2 g/kg) intraperitoneal injection. (C) Intraperitoneal glucose tolerance tests (IPGTT) in male (left) and female (right) TG mice that had normal blood glucose levels in fasted and fed states at 8 weeks of age. (D) Insulin tolerance test (ITT) in male (left) and female (right) TG and control mice at 12 weeks of age. (E) Plasma insulin (left) and glucagon (right) levels in fasted and fed conditions in TG and control mice at 12 weeks of age. (F) The size-matched islets were isolated from male TG and control mice at 12 weeks of age, and glucose-stimulated insulin secretion levels were measured in those islets. The data were normalized by DNA content in those islets (G and H) TG mice that had normal blood glucose levels at 4 weeks of age and their control littermates were fed a HFHSD for 8 weeks further, then, fasted blood glucose levels were measured (G) and IPGTT performed (H) in both male (left) and female (right) mice. In each experiment (A–H), at least six male or female mice for each genotype were analyzed. In the line graphs, closed triangle indicates TG mice and open circle indicates control mice. Data represent mean ± SEM. * or ** indicates <i>P</i><0.05 or <i>P</i><0.01 by ANOVA.</p

FoxO1-ADA expression and the knockdown of FoxO1 affect Chrebp O-glycosylation, protein stability and transcriptional activity in primary hepatocytes.

<p>(A, B, D and E) Mouse primary hepatocytes infected with adenovirus expressing FoxO1-ADA or LacZ were cultured with 5 mM or 25 mM glucose. The cell lysates were subjected to real-time RT-PCR for Chrebp or L-PK (A), immunoprecipitation with anti-Chrebp antibody followed by blotting with anti-O-GlcNAc antibody (B) or anti-ubiquitin antibody (D). The cell lysates were also subjected to chromatin-immunoprecipitation assay using anti-Chrebp antibody and the primers for L-PK promoter (E). (C) Mouse primary hepatocytes infected with adenovirus expressing specific siRNA for FoxO1 or control siRNA were cultured with 5 mM or 25 mM glucose and the cell lysates were immunoprecipitated with anti-Chrebp antibody followed by blotting with anti-O-GlcNAc antibody. Input represents expression levels of Chrebp, FoxO1-ADA, endogenous FoxO1 and Tubulin. Quantitative analyses were performed by assessment of O-glycosylation level compared with the protein level of Chrebp using densitometry, showing as a bar graph below the results of blotting (B and C). (F) Mouse primary hepatocytes co-transfected with pGL3-3Xl-PK-ChoRE and OGT or LacZ were infected with adenovirus expressing FoxO1-ADA or LacZ at indicated MOI and cultured with 5 mM or 25 mM glucose for 24 hr. The cell lysates were used for luciferase assays. Experiments were repeated at least three times. Data represent mean ± SEM. *P<0.05.</p

Islet vasculature and VEGF-A expression in β cells are increased in TG mice via FoxO1 regulation of VEGF-A transcription.

<p>(A) Hematoxylin and eosin (HE) staining and immunohistochemistry with anti-PECAM1 or anti-VEGF-A antibodies in pancreatic sections from 2 month old TG and control mice. Representative images are shown. (B) Islets were isolated from TG and control mice and used for analyses by real-time RT-PCR to quantify PECAM1 and VEGF-A mRNA levels. In each experiment (A and B), six male and six female mice were analyzed for each genotype. There was no difference between male and female mice. Data represent the mean ± SEM fold-increase relative to control mice. An asterisk indicates <i>P</i><0.05 by ANOVA. (C) β TC3 or MIN6 cells were infected with adenoviruses expressing FoxO1 ADA or GFP at indicated MOI, 24 hrs later mRNA was isolated from the cells and used for real-time RT-PCR to quantify VEGF-A mRNA levels. The results of (B) and (C) were normalized using GAPDH. (D) VEGF-A promoter driven luciferase activities were measured in MIN6 cells infected with adenoviruses expressing FoxO1 ADA or GFP at indicated MOI. For (C) and (D), data represent the mean ± SEM fold-increase relative to GFP infection. An asterisk indicates <i>P</i><0.05 by ANOVA. (E) The promoter region of the mouse VEGF-A gene. Square indicates the location of the forkhead responsive element (FHRE). For ChIP assays, a primer set spanning three FHREs and control primers were used. An arrow indicates the initiation site of RNA synthesis and is designated +1. (F) ChIP assays of the VEGF-A promoter are shown. We performed ChIP assays in MIN6 cells with indicated antisera. NRS indicates normal rabbit serum used as a control antisera. The crosslinked DNA was amplified by PCR using FHRE and Cont primers. Input represents DNA extracted from chromatin prior to immunoprecipitation. We used input DNA with 1∶100 dilution.</p

Chrebp O-glycosylation is regulated by OGT.

<p>(A) Mouse primary hepatocytes were transfected with Flag-Chrebp and labeled with tetraacetylated azide-modified N-acetylglucosamine (GlcNAz). The cell lysates were immunopricipitated with anti-Flag antibody or normal mouse globlin (NMG) and subjected to detection of O-glycosylation by biothin-avidin system as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047231#s2" target="_blank">Materials and Methods</a>. Input indicates the expression level of Flag-Chrebp in each lane. (B and C) Mouse primary hepatocytes were transfected with HA-OGT (B) or siRNA for OGT (C) along with empty vector or control siRNA, respectively and cultured with 5 mM or 25 mM glucose for 24 hr. In some experiments, 10 mM glucosamine (GlcN) was added in the medium. The cell lysates were immunoprecipitated with anti-Chrebp antibody followed by blotting with anti-O-GlcNAc or anti-HA antibody. Input indicates the expression level of Chrebp, HA-OGT, endogenous OGT or tubulin. Quantitative analyses were performed by assessment of O-glycosylation level compared with the protein level of Chrebp using densitometry, showing as a bar graph below the results of blotting (B and C). Experiments were repeated at least three times. Data represent mean ± SEM. *P<0.05.</p