Cortical regions superimposed on the T2-weighted MRI template.

<p>Insular region is ventral and not shown in the figure.</p

Regional Volume Decreases in the Brain of <i>Pax6</i> Heterozygous Mutant Rats: MRI Deformation-Based Morphometry - Fig 5

<p><b>(A) Expression pattern of <i>Pax6</i> in rat embryo at E10.5.</b> The figure was modified from Kikkawa et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158153#pone.0158153.ref050" target="_blank">50</a>]. dte, dorsal telencephalon; di, diencephalon; me, mesencephalon; rh, rhombencephalon; vte, ventral telencephalon. <b>(B) Pax6 expression in an E14.5 mouse cortex.</b> Pax6 protein (red) is restricted to the ventricular zone (VZ), where neural progenitor cells reside; in contrast, <i>Pax6</i> is not expressed in the Tuj1-positive (blue) cortical plate (CP). The figure was modified from Osumi and Kikkawa [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158153#pone.0158153.ref051" target="_blank">51</a>]. <b>(C) Expression pattern of <i>Pax6</i> in the adult mouse based on previously published data (Duan et al., 2013</b> [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158153#pone.0158153.ref041" target="_blank">41</a>]; <b>Haba et al., 2009</b> [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158153#pone.0158153.ref052" target="_blank">52</a>]; <b>Kohwi et al., 2005</b> [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158153#pone.0158153.ref053" target="_blank">53</a>]; <b>Maekawa et al., 2005</b> [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158153#pone.0158153.ref054" target="_blank">54</a>]). The regions with <i>Pax6</i> expression in the studies of Duan et al. and Haba et al. are shown in green, and the regions in which <i>Pax6</i> was expressed in neural stem cells/progenitor cells in the other studies are shown in red. Nomenclature and illustration of various brain regions are based on <i>The Mouse Brain in Stereotaxic Coordinates</i> (2^nd Edition) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158153#pone.0158153.ref055" target="_blank">55</a>]. AA, anterior amygdaloid area; AO, anterior olfactory nucleus; APT, anterior pretectal nucleus; BM/BL, basomedial/basolateral amygdaloid nucleus; Ce, central amygdaloid nucleus; CG, central gray; DG, hippocampal dentate gyrus; DR, dorsal raphe nucleus; EP, entopeduncular nucleus; EPl, external plexiform layer of the olfactory bulb; F, nucleus of the fields of Forel; GCL, granular cell layer of the cerebellum; Gl, glomerular layer of the olfactory bulb; Gr, granule cell layer of the olfactory bulb; Hb, habenular nucleus; I, intercalated nuclei of the amygdala; InC, interstitial nucleus of Cajal; LH, lateral hypothalamic area; LL, nucleus of the lateral lemniscus; LPO, lateral preoptic area; LSI, lateral septal nucleus, intermediate part; MCPC, magnocellular nucleus of the posterior commissure; Me, Medial amygdaloid nucleus; mRt, mesencephalic reticular formation; MS, medial septal nucleus; PAG, periaqueductal gray; PCom, nucleus of the posterior commissure; PDTg/LDTg, posterodorsal/laterodorsal tegmental nucleus; Pir, Piriform cortex; PL, paralemniscal nucleus; Pn, pontine nuclei; PrC, precommissural nucleus; Rt, reticular thalamic nucleus; RtTg, reticulotegmental nucleus of the pons; SubG, subgeniculate nucleus; SuVe/MVe, superior/medial vestibular nucleus; SPFPC, subparafascicular thalamic nucleus, parvicellular part; SVZ, subventricular zone; Tu, olfactory tubercle; VC/DC, ventral/dorsal cochlear nucleus; VDB/HDB, nucleus of the vertical/horizontal limb of the diagonal band; VP, ventral pallidum; VTT/DTT, ventral/dorsal tenia tecta; X, nucleus X; ZI, zona incerta.</p

Volumes of the neocortical regions in the ROI analysis.

<p>Volumes of the neocortical regions in the ROI analysis.</p

Volumes of brain regions in the ROI analysis.

<p>Volumes of brain regions in the ROI analysis.</p

Regional volume decrease in the brain of <i>rSey</i>^<i>2</i>/+ rats compared to WT rats.

<p>Colored voxels represent clusters of significant regional volume decrease in the brain of <i>rSey</i>^<i>2</i>/+ rats (n = 29) compared to WT rats (n = 31) in ANOVA (FWE, <i>p</i> < 0.05; threshold of 500 voxels) superimposed on the T2-weighted MRI template. rSey²/+, Pax6 heterozygous mutant; WT, wild-type; ANOVA; analysis of variance; FWE, family-wise error.</p

Regional volume decreases in the brain of male <i>rSey</i>^<i>2</i>/+ rats compared to male WT rats.

<p>Colored voxels represent clusters of significant regional volume decreases in the brain of male <i>rSey</i>^<i>2</i>/+ rats (n = 16) compared to male WT rats (n = 16) in the post hoc analysis after ANOVA (FWE, <i>p</i> < 0.05, threshold of 500 voxels), superimposed on the T2-weighted MRI template. rSey²/+, Pax6 heterozygous mutant; WT, wild-type; ANOVA; analysis of variance; FWE, family-wise error.</p

Number of voxels in each neocortical region which showed significant volume decrease in <i>rSey</i>^<i>2</i>/+ rats compared to WT rats in voxel-by-voxel analysis (FWE, p < 0.05; threshold of 500 voxels).

<p>Number of voxels in each neocortical region which showed significant volume decrease in <i>rSey</i>^<i>2</i>/+ rats compared to WT rats in voxel-by-voxel analysis (FWE, p < 0.05; threshold of 500 voxels).</p

Number of voxels in each region which showed significant volume decrease in <i>rSey</i>^<i>2</i>/+ rats compared to WT rats in voxel-by-voxel analysis (FWE, p < 0.05; threshold of 500 voxels).

<p>Number of voxels in each region which showed significant volume decrease in <i>rSey</i>^<i>2</i>/+ rats compared to WT rats in voxel-by-voxel analysis (FWE, p < 0.05; threshold of 500 voxels).</p