Nonthermal X-radiation of SNR RX J1713.7-3946: The Relations to a Nearby Molecular Cloud

The recent X-ray and CO observations of RX J1713.7-3946 show that a significant fraction of the nonthermal X-ray emission of this unique supernova remnant associates, in one way or another, with a molecular cloud interacting with the west part of the shell. This adds a new puzzle in the origin of X-ray emission which cannot be easily explained within the standard model in accordance of which X-rays are result of synchrotron radiation of multi-TeV electrons accelerated by supernova shock waves. We explore an alternative origin of the X-ray emission assuming that it is produced by secondary electrons resulting from high energy hadronic interactions in the molecular gas. Such a scenario could explain in a quite natural way the apparent correlation between the X-ray and CO morphologies. However, the TeV gamma-ray emission recently reported by H.E.S.S. significantly constrains the parameter space of this model. Namely, this mechanism cannot reproduce the bulk of the observed X-ray flux unless one postulates existence of a PeV cosmic-ray component penetrating with an unusually hard spectrum into the dense cloud.Comment: 6 pages, 3 figures, to appear in Proc. of Int. Symp. on High Energy Gamma-ray Astronomy, Heidelberg (July 2004

Chandra Observations of A Galactic Supernova Remnant Vela Jr.: A New Sample of Thin Filaments Emitting Synchrotron X-Rays

A galactic supernova remnant (SNR) Vela Jr. (RX J0852.0$-$4622, G266.6$-$1.2) shows sharp filamentary structure on the north-western edge of the remnant in the hard X-ray band. The filaments are so smooth and located on the most outer side of the remnant. We measured the averaged scale width of the filaments ($w_u$ and $w_d$) with excellent spatial resolution of {\it Chandra}, which are in the order of the size of the point spread function of {\it Chandra} on the upstream side and 49.5 (36.0--88.8) arcsec on the downstream side, respectively. The spectra of the filaments are very hard and have no line-like structure, and were well reproduced with an absorbed power-law model with $\Gamma =$2.67 (2.55--2.77), or a {\tt SRCUT} model with $\nu_{rolloff}$ = 4.3 (3.4--5.3)$\times 10^{16}$ Hz under the assumption of $p=0.3$. These results imply that the hard X-rays are synchrotron radiation emitted by accelerated electrons, as mentioned previously. Using a correlation between a function ${\cal B} \equiv \nu_{rolloff}/w_d^2$ and the SNR age, we estimated the distance and the age of Vela Jr.: the estimated distance and age are 0.33 (0.26--0.50) kpc and 660 (420--1400) years, respectively. These results are consistent with previous reports, implying that ${\cal B}$--age relation may be a useful tool to estimate the distance and the age of synchrotron X-ray emitting SNRs.Comment: 19 pages, 8 figures, ApJ, in pres

Search for Sc-K line emission from RX J0852.0--4622 Supernova remnant with Suzaku

We searched for evidence of line emission around 4keV from the northwestern rim of the supernova remnant RX J0852.0-4622 using Suzaku XIS data. Several papers have reported the detection of an emission line around 4.1keV from this region of the sky. This line would arise from K-band fluorescence by Sc, the immediate decay product of 44Ti. We performed spectral analysis for the entire portion of the NW rim of the remnant within the XIS field of view, as well as various regions corresponding to regions of published claims of line emission. We found no line emission around 4.1keV anywhere, and are able to set a restrictive upper limit to the X-ray flux: 1.1x10^-6 s^-1 cm^-2 for the entire field. For every region, our flux upper limit falls below that of the previously claimed detection. Therefore, we conclude that, to date, no definite X-ray line feature from Sc-K emission has been detected in the NW rim of RX J0852.0-4622. Our negative-detection supports the recent claim that RX J0852-4622 is neither young (1700--4000 yr) nor nearby(~750 pc).Comment: Published in PAS

Suzaku X-Ray Imaging and Spectroscopy of Cassiopeia A

Suzaku X-ray observations of a young supernova remnant, Cassiopeia A, were carried out. K-shell transition lines from highly ionized ions of various elements were detected, including Chromium (Cr-Kalpha at 5.61 keV). The X-ray continuum spectra were modeled in the 3.4--40 keV band, summed over the entire remnant, and were fitted with a simplest combination of the thermal bremsstrahlung and the non-thermal cut-off power-law models. The spectral fits with this assumption indicate that the continuum emission is likely to be dominated by the non-thermal emission with a cut-off energy at > 1 keV. The thermal-to-nonthermal fraction of the continuum flux in the 4-10 keV band is best estimated as ~0.1. Non-thermal-dominated continuum images in the 4--14 keV band were made. The peak of the non-thermal X-rays appears at the western part. The peak position of the TeV gamma-rays measured with HEGRA and MAGIC is also shifted at the western part with the 1-sigma confidence. Since the location of the X-ray continuum emission was known to be presumably identified with the reverse shock region, the possible keV-TeV correlations give a hint that the accelerated multi-TeV hadrons in Cassiopeia A are dominated by heavy elements in the reverse shock region.Comment: Publ. Astron. Soc. Japan 61, pp.1217-1228 (2009

Enhanced bone-forming activity of side population cells in the periodontal ligament.

Regeneration of alveolar bone is critical for the successful treatment of periodontal diseases. The periodontal ligament (PDL) has been widely investigated as a source of cells for the regeneration of periodontal tissues. In the present study where we attempted to develop an effective strategy for alveolar bone regeneration, we examined the osteogenic potential of side population (SP) cells, a stem cell-containing population that has been shown to be highly abundant in several kinds of tissues, in PDL cells. Isolated SP cells from the rat PDL exhibited a superior ability to differentiate into osteoblastic cells compared with non-SP (NSP) and unsorted PDL cells in vitro. The mRNA expressions of osteoblast markers and bone morphogenetic protein (BMP) 2 were significantly upregulated in SP cells and were further increased by osteogenic induction. To examine the boneforming activity of SP cells in vivo, PDL SP cells isolated from green fluorescent protein (GFP)-transgenic rats were transplanted with hydroxyapatite (HA) disks into wild-type animals. SP cells exhibited a high ability to induce the mineralized matrix compared with NSP and unsorted PDL cells. At 12 weeks after the implantation, some of the pores in the HA disks with SP cells were filled with mineralized matrices, which were positive for bone matrix proteins, such as osteopontin, bone sialoprotein, and osteocalcin. Furthermore, osteoblast- and osteocyte-like cells on and in the bone-like mineralized matrices were GFP positive, suggesting that the matrices were directly formed by the transplanted cells. These results suggest that PDL SP cells possess enhanced osteogenic potential and could be a potential source for cell-based regenerative therapy for alveolar bone

Designing libraries of chimeric proteins using SCHEMA recombination and RASPP

SCHEMA is a method for designing libraries of novel proteins by recombination of homologous sequences. The goal is to maximize the number of folded proteins while simultaneously generating significant sequence diversity. Here, we use the RASPP algorithm to identify optimal SCHEMA designs for shuffling contiguous elements of sequence. To exemplify the method, SCHEMA is used to recombine five fungal cellobiohydrolases (CBH1s) to produce a library of more than 390,000 novel CBH1 sequences

Superpositioning of Deletions Promotes Growth of Escherichia coli with a Reduced Genome

Escherichia coli has dispensable genome regions and eliminating them may improve cell use by reducing unnecessary metabolic pathways and complex regulatory networks. Although several strains with reduced genomes have already been constructed, there have been no reports of strains constructed with deletions assayed for influence on growth. To retain robust growth and fundamental metabolic pathways, the growth of each deletion strain and combination effects of deletions were checked using M9 minimal medium. Then a new strain, MGF-01, with a 1 Mb reduced genome was constructed by integrating deletions that did not affect growth. MGF-01 grew as well as the wild type in the exponential phase and continued growing after the wild type had entered the stationary phase. The final cell density of MGF-01 was 1.5 times higher than that of the wild-type strain. Using MGF-01 as a production host, a 2.4-fold increase in l-threonine production was achieved