Formation and Electrical Evaluation of a Single Metallized DNA Nanowire in a Nanochannel

A novel fabrication process was developed for a single silver nanowire using DNA metallization in a nanochannel, and the electrical properties of this nanowire were evaluated using electrochemical impedance spectroscopy. After being isolated using a nanochannel measuring 500 nm in depth and 500 nm in width, a single λDNA molecule was electrostatically stretched and immobilized between two electrodes separated by a gap of 15 μm by applying an AC voltage of 1 MHz and 20 Vp‐p. Then, naphthalene diimide molecules terminally‐labeled with galactose moieties were intercalated into the λDNA, and the reduction of silver ions along the λDNA led to its metallization with silver. Scanning electron microscopy observations revealed that two nanowires having different average widths of 154 nm and 250 nm were formed in two individual nanochannels. The nanowires showed the linear current‐voltage characteristics, and their combined resistance was estimated to be 45.5 Ω. The complex impedance of the nanowires was measured, and an equivalent circuit was obtained as a series connection of a resistance and a parallel resistance‐constant phase element circuit. Impedance analysis revealed that the nanowire included silver grain boundaries, and the bulk resistivity of silver grain was estimated to be 8.35×10−8 Ωm

Improvement of forage quality by means of molecular breeding in tropical grasses

Many important warm- season perennial grasses multiply either by vegetative propagation, or form their seeds by an asexual mode of reproduction called apomixes. Possibility of improving of these plants by conventional breeding method depends on availability of natural genetic variation and its manipulation through breeding and selection. However, there are naturally not many genetic variations in apomictic grasses to generate new genetic variation. Plant tissue culture techniques have complemented conventional plant breeding programs. The author established a system for plant regeneration from in vitro-cultured calluses, suspension cells and protoplasts in some tropical grass species including apomictic species. In addition, we focus on describing current and future applications and impact of genetic transformation in tropical grasses

Promotion of a venous thromboembolism prevention protocol at a perioperative management center

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Formation and Electrical Evaluation of a Single Metallized DNA Nanowire in a Nanochannel

A novel fabrication process was developed for a single silver nanowire using DNA metallization in a nanochannel, and the electrical properties of this nanowire were evaluated using electrochemical impedance spectroscopy. After being isolated using a nanochannel measuring 500 nm in depth and 500 nm in width, a single λDNA molecule was electrostatically stretched and immobilized between two electrodes separated by a gap of 15 μm by applying an AC voltage of 1 MHz and 20 Vp‐p. Then, naphthalene diimide molecules terminally‐labeled with galactose moieties were intercalated into the λDNA, and the reduction of silver ions along the λDNA led to its metallization with silver. Scanning electron microscopy observations revealed that two nanowires having different average widths of 154 nm and 250 nm were formed in two individual nanochannels. The nanowires showed the linear current‐voltage characteristics, and their combined resistance was estimated to be 45.5 Ω. The complex impedance of the nanowires was measured, and an equivalent circuit was obtained as a series connection of a resistance and a parallel resistance‐constant phase element circuit. Impedance analysis revealed that the nanowire included silver grain boundaries, and the bulk resistivity of silver grain was estimated to be 8.35×10−8 Ωm