1 research outputs found
Cloning, Sequence Analysis and Expression Patterns during Seed Germination of a Rapeseed (Brassica napus L.) G-x-S-x-G-motif Lipase Gene
[EN] Lipases catalyze the hydrolysis of ester bonds in triacylglycerides, generating glycerol and free fatty acids. These enzymes are
encoded by extremely complex gene families, and appear to fulfil many different biological functions. Although they are
present in all types of organisms, available information on plant lipases is still very limited, as compared to their bacterial and
animal counterparts. A full-length clone, BnLIP, encoding a putative lipase, has been isolated by PCR amplification of Brassica
napus genomic DNA, with oligonucleotide primers derived from the sequence of an Arabidopsis thaliana homologue. The
clone included an open reading frame of 1581 bp encoding a polypeptide of 526 amino acids, with a calculated molecular mass
of 59.5 kDa. Analysis of the deduced protein sequence, sequence alignment with homologous proteins from related plant
species, and a phylogenetic analysis revealed that the BnLIP protein belongs to the classical GxSxG-motif lipase family. RTPCR
assays indicated that the BnLIP gene is expressed specifically, but only transiently, during seed germination: the lipase
mRNA was not present at detectable levels in ungerminated seeds, was detected only three days after seed imbibition, but its
levels decreased rapidly afterwards. No expression was observed in roots, stems or leaves of adult plants. This expression pattern
suggests that BnLIP is one of the lipases involved in the hydrolysis of triacylglycerides stored in rapeseed seeds, ultimately
providing nutrients and energy to sustain seedling growth until photosynthesis is activatedGlaied Ghram, I.; Belguith, H.; Ben Mustapha, M.; Himila, I.; Bouhaouala, B.; Vicente, O.; Ben Hamida, J. (2016). Cloning, Sequence Analysis and Expression Patterns during Seed Germination of a Rapeseed (Brassica napus L.) G-x-S-x-G-motif Lipase Gene. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 44(2):437-444. doi:10.15835/nbha4421047243744444