8 research outputs found
Identification of the P-TEFb complex-interacting domain of Brd4 as an inhibitor of HIV-1 replication by functional cDNA library screening in MT-4 cells
Get PDFAbstractWe conducted a phenotypic cDNA screening using a T cell line-based assay to identify human genes that render cells resistant to human immunodeficiency virus type 1 (HIV-1). We isolated potential HIV-1 resistance genes, including the carboxy terminal domain (CTD) of bromodomain-containing protein 4 (Brd4). Expression of GFP-Brd4-CTD was tolerated in MT-4 and Jurkat cells in which HIV-1 replication was markedly inhibited. We provide direct experimental data demonstrating that Brd4-CTD serves as a specific inhibitor of HIV-1 replication in T cells. Our method is a powerful tool for the identification of host factors that regulate HIV-1 replication in T cells
The Conserved 12-Amino Acid Stretch in the Inter-Bromodomain Region of BET Family Proteins Functions as a Nuclear Localization Signal
No full textA Cell-Based, Microplate Colorimetric Screen Identifies 7,8-Benzoflavone and Green Tea Gallate Catechins as Inhibitors of the Hepatitis C Virus
No full textSynthesis and biological evaluation of benzamides and benzamidines as selective inhibitors of VEGFR tyrosine kinases
No full textInhibition of Anchorage-Independent Growth ofras-Transformed Cells on PolyHEMA Surface by Antisense Oligodeoxynucleotides Directed Against K-ras
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