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京都大学0048新制・課程博士博士(医学)甲第14100号医博第3275号新制||医||970(附属図書館)UT51-2008-L155京都大学大学院医学研究科内科系専攻(主査)教授 稲垣 暢也, 教授 芹川 忠夫, 教授 瀬原 淳子学位規則第4条第1項該当Doctor of Medical ScienceKyoto UniversityDA

A New in vitro Invasion Model for Oral Cancer Using an Acellular Allogenic Dermal Matrix (Alloderm) : The Relationship among in vitro Invasion Activity, in vivo Invasion and Metastasis

The purpose of this study was to develop a new in vitro model of cancer invasion using a human dermal equivalent, AlloDerm®. A squamous cell carcinoma line (HSC-2, HSC-3 and OSC20) and a malignant melanoma line (G361) were cultured on AlloDerm® and evaluated histologically to examine destruction of the basement membrane and invasion into the dermis of AlloDerm®. HSC-3 and G361 cells invaded through the basement membrane of AlloDerm® at 21-28 days after seeding, while HSC-2 and OSC20 cells did not. Next, these cells were transplanted into the tongues of nude mice, and the degree of local invasiveness was examined histologically. HSC-3 and G361 cells invaded diffusely to the surrounding tongue tissue, although HSC-2 and OSC20 showed only expansive growth. Further, these cells were transplanted subcutaneously in nude mice to study metastatic activity. Regional and distant metastases were rarely observed after transplantation of HSC-2 and OSC20 cells. On the other hand, HSC-3 and G361 cells frequently metastasized. These findings show a close relationship among in vitro invasiveness on AlloDerm®, in vivo invasiveness, and metastatic activity. This experimental model using AlloDerm® is a potentially new in vitro model of cancer invasion

A New in Vitro Model of Cancer Invasion Using AlloDermR, a Human Cadaveric Dermal Equivalent: a Preliminary Report

Objectives. The first stage in the metastasis of squamous cell carcinoma (SCC) of the oral cavity involves the destruction of the basement membrane and invasion into the submucosal tissue. The purpose of this study is to develop a new in vitro model of cancer invasion using a human dermal equivalent, AlloDermR. Study design. Normal epithelial cells from a gingiva and a SCC line originating from human tongue cancer (HSC-3 and 4 cells) were cultured on AlloDermR, and composites of these cells and AlloDermR were evaluated histologically by HE staining and immunostaining with anti-laminin and type IV collagen antibodies. Furthermore, HSC-3 and 4 cells were transplanted into the tongues of nude mice, and regional lymph node metastases were examined histologically. Results. HSC-3 cells had invaded through the basement membrane into the AlloDermR dermis at 25 days after seeding. Decreases in the levels of laminin and type IV collagen were observed in the locations where HSC-3 had invaded. Metastasis to regional lymph nodes was observed at 3 weeks after transplantation in 4 of 10 (40%) mice. On the other hand, normal epithelial cells and HSC-4 cells did not show invasion into AlloDermR. Lymph node metastasis was not observed in the mice bearing HSC-4 cells. Conclusion. This experimental model using AlloDermR is a potential new in vitro model of cancer invasion

Absence of the Sternocleidomastoid Muscle in a Patient That Underwent Neck Dissection for Squamous Cell Carcinoma of the Tongue

The congenital absence of a skeletal muscle is a rare cause of congenital muscular torticollis, and the condition is associated with various unusual anatomical structures. We describe a rare case of congenital absence of the sternocleidomastoid muscle associated with squamous cell carcinoma of the tongue in a patient who underwent neck dissection. In this case, both the external jugular vein and the spinal accessory nerve were absent. However, we found that branches of the C3 nerve extended from the cervical plexus to the trapezius muscle and seemed to be acting as trapezius muscle motor nerves in place of the accessory nerve. After the operation, the patient was able to lift and abduct his right arm, and his shoulder did not droop

The effect of mountaineering on the association between blood pressure and physical activity: A new multi‐sensor ambulatory blood‐pressure monitoring device. The Mount Fuji Study

Abstract It has not been fully investigated whether the response of blood pressure (BP) to activity at high altitudes differs from that at low altitudes or how temperature is involved in these differences. The author compared BP response to accelerometer measurements during mountaineering and daily living. In 15 healthy people (mean age 33 ± 6 years), a new multi‐sensor ambulatory BP monitoring (ABPM) device equipped with barometer, thermometer, and accelerometer was used to measure BP responses to activity during a trip to Mt. Fuji and during daily living. Associations between physical activity (log‐transformed 5‐min average values of accelerometer just before each ambulatory BP) and the corresponding BP were obtained from 843 and 676 readings during the Mt. Fuji trip and daily living, respectively. All ambulatory systolic BP (SBP) parameters were significantly higher during the Mt. Fuji trip than during daily living (all p < .01). There were significant positive correlations between physical activity and corresponding BPs in both mountaineering and daily living (all p < .01), and there was an interaction between BPs and physical activity according to the two conditions (p < .01). On Mt. Fuji, multivariate regression analysis showed increased physical activity and lower temperature were associated with increased 24‐h SBP and diastolic BP (DBP) (all p < .05). The goodness‐of‐fit values of the association between activity and 24‐h SBP or DBP were improved by adding temperature to the model of both 24‐h SBP and DBP. However, these associations were not found in the daily living model. BP response to activity was more pronounced during mountaineering than daily living