16 research outputs found
A temporal shift of the evolutionary principle shaping intratumor heterogeneity in colorectal cancer
Advanced colorectal cancer harbors extensive intratumor heterogeneity shaped by neutral evolution; however, intratumor heterogeneity in colorectal precancerous lesions has been poorly studied. We perform multiregion whole-exome sequencing on ten early colorectal tumors, which contained adenoma and carcinoma in situ. By comparing with sequencing data from advanced colorectal tumors, we show that the early tumors accumulate a higher proportion of subclonal driver mutations than the advanced tumors, which is highlighted by subclonal mutations in KRAS and APC. We also demonstrate that variant allele frequencies of subclonal mutations tend to be higher in early tumors, suggesting that the subclonal mutations are subject to selective sweep in early tumorigenesis while neutral evolution is dominant in advanced ones. This study establishes that the evolutionary principle underlying intratumor heterogeneity shifts from Darwinian to neutral evolution during colorectal tumor progression
Thrombotic thrombocytopenic purpura in a patient with a diffuse form of systemic sclerosis
Decreased Expression of Fructose-1,6-bisphosphatase Associates with Glucose Metabolism and Tumor Progression in Hepatocellular Carcinoma
Characterization and distribution of two subtypes of Verticillium longisporum isolated from cabbage fields in Japan
Hierarchical clustering showing the expression levels of the differentially expressed genes in a comparison of CRC patients with the pre-<i>miR-146a</i>/C (CC/CG) genotype and without the pre-<i>miR-146a</i>/C (GG) genotype.
<p>Red spots indicate upregulated and blue spots indicate downregulated probes compared with reference probes. On the top, clustering results of CRC patients are shown (dendrogram). Red bar indicates the CC/CG genotype and the blue bar indicates the GG genotype. On the left side, clustering results of the differentially expressed genes between the two genotypes are shown.</p
<i>miR-146a</i> Polymorphism (rs2910164) Predicts Colorectal Cancer Patients’ Susceptibility to Liver Metastasis - Fig 4
<p>Downregulated <i>miR-146a</i> (A) and upregulated NUMB (B) expression of CRC cell lines with pre-miR-146a/C transfected with miR-146a inhibitor or negative control.</p
Comparative analysis of the clinicopathological findings affected by miR-146a polymorphism.
<p>Comparative analysis of the clinicopathological findings affected by miR-146a polymorphism.</p
<i>miR-146a</i> Polymorphism (rs2910164) Predicts Colorectal Cancer Patients’ Susceptibility to Liver Metastasis - Fig 2
<p>Gene Set Enrichment Analysis (GSEA): Enriched gene sets for CRC patients with the C allele (CC/CG); KEGG_NOTCH_SIGNALING_PATHWAY (A) and V$STAT3_01 (B).</p
Genotyping of miR-146a polymorphism in 7 CRC cell lines.
<p>Genotyping of miR-146a polymorphism in 7 CRC cell lines.</p