Protein Transduction Method for Cerebrovascular Disorders

Many studies have shown that a motif of 11 consecutive arginines (11R) is one of the most effective protein transduction domains (PTD) for introducing proteins into the cell membrane. By conjugating this &#34;11R&#34;, all sorts of proteins can effectively and harmlessly be transferred into any kind of cell. We therefore examined the transduction efficiency of 11R in cerebral arteries and obtained results showing that 11R fused enhanced green fluorescent protein (11R-EGFP) immediately and effectively penetrated all layers of the rat basilar artery (BA), especially the tunica media. This method provides a revolutionary approach to cerebral arteries and ours is the first study to demonstrate the successful transductionof a PTD fused protein into the cerebral arteries. In this review, we present an outline of our studies and other key studies related to cerebral vasospasm and 11R, problems to be overcome, and predictions regarding future use of the 11R protein transduction method for cerebral vasospasm (CV).</p

Elimination of metal interference in the determination of fluoride ion by non-suppressor type ion chromatography

A simple method has been developed to eliminate metal interferences in the ion-chromatographic determination of fluoride ion in aqueous solutions. Negative interferences become appreciable on 0.2-mM F at 0.01 to 0.02 mM of Al(III), Ce(IV), La(III), Y(III), Ce(III) and Pb(II); and at 0.25 mM of Ca(II). The interference from Cd(II), Co(II), Fe(II) and Ni(II) is insignificant at ≤ 5.0-mM metal for 5.0-mM F-. By alkalifying F- solutions at pH 12.3, the metal interference can be eliminated up to the concentrations of 0.25-mM Al(III), La(III) and Y(III); 1.0-mM Ce(IV); 2.5-mM Ce(III); and 5.0-mM Pb(II), Cd(II), Co(II), Fe(II) and Ni(II). The Ca(II) interference cannot be eliminated. The pretreatment enables determination of 0.2- to 5.0-mM F- in presence of 0.25-mM Al(III) or La(III) with coefficient-of-variations of 1.99 to 6.20%

Malignant peripheral nerve sheath tumor arising from the greater omentum: Case report

Malignant peripheral nerve sheath tumors (MPNSTs) are rare soft tissue tumors that arise from a peripheral nerve or exhibit nerve sheath differentiation. Most of these tumors arise on the trunk, extremities, or head and neck regions; they are very rarely located in the abdominal cavity. The patient was a 71-year-old man who was referred to our hospital for a mass and pain in the right lower abdomen. Abdominal computed tomography revealed a large (9 × 9 cm), well-circumscribed, lobulated, heterogeneously enhanced mass in the pelvis. Exploratory laparotomy revealed a large mass in the greater omentum, and the tumor was completely excised. Histopathological analysis revealed that the tumor was composed of spindle cells with high mitotic activity. On staining the tumor, positive results were obtained for S-100 but negative results were obtained for c-kit, cluster of differentiation (CD)34, α-smooth muscle actin, and desmin. These findings strongly supported a diagnosis of MPNST primarily arising from the greater omentum. To the best of our knowledge, this is the first reported case of an MPNST arising from the greater omentum. In this report, we have described the case of a patient with an MPNST arising from the greater omentum and have discussed the clinical characteristics and management of MPNSTs

H2_2 Emission Nebulosity Associated with KH 15D

An H$_2$ emission filament is found in close proximity to the unique object KH 15D using the adaptive optics system of the Subaru Telescope. The morphology of the filament, the presence of spectroscopic outflow signatures observed by Hamilton et al., and the detection of extended H$_2$ emission from KH 15D by Deming, Charbonneau, & Harrington suggest that this filament arises from shocked H$_2$ in an outflow. The filament extends about 15" to the north of KH 15D.Comment: 11 pages, 3 figures, 1 table. Astrophysical Journal Letters, in pres

OTUD1 deubiquitinase regulates NF-κB- and KEAP1-mediated inflammatory responses and reactive oxygen species-associated cell death pathways

Deubiquitinating enzymes (DUBs) regulate numerous cellular functions by removing ubiquitin modifications. We examined the effects of 88 human DUBs on linear ubiquitin chain assembly complex (LUBAC)-induced NF-κB activation, and identified OTUD1 as a potent suppressor. OTUD1 regulates the canonical NF-κB pathway by hydrolyzing K63-linked ubiquitin chains from NF-κB signaling factors, including LUBAC. OTUD1 negatively regulates the canonical NF-κB activation, apoptosis, and necroptosis, whereas OTUD1 upregulates the interferon (IFN) antiviral pathway. Mass spectrometric analysis showed that OTUD1 binds KEAP1, and the N-terminal intrinsically disordered region of OTUD1, which contains an ETGE motif, is indispensable for the KEAP1-binding. Indeed, OTUD1 is involved in the KEAP1-mediated antioxidant response and reactive oxygen species (ROS)-induced cell death, oxeiptosis. In Otud1−/−-mice, inflammation, oxidative damage, and cell death were enhanced in inflammatory bowel disease, acute hepatitis, and sepsis models. Thus, OTUD1 is a crucial regulator for the inflammatory, innate immune, and oxidative stress responses and ROS-associated cell death pathways

Near-Infrared Adaptive Optics Spectroscopy of Binary Brown Dwarf HD 130948B and C

We present near-infrared spectroscopy of low-mass companions in a nearby triple system HD 130948 (Gliese 564, HR 5534). Adaptive optics on the Subaru Telescope allowed spectroscopy of the individual components of the 0".13 binary system. Based on a direct comparison with a series of template spectra, we determined the spectral types of HD 130948B and C to be L4 +- 1. If we take the young age of the primary star into account (0.3-0.8 Gyr), HD 130948B and C most likely are a binary brown dwarf system.Comment: 6 pages, 3 figures, accepted for publication in ApJ Letter

A Subarcsecond Companion to the T Tauri Star AS 353B

Adaptive optics imaging of the bright visual T Tauri binary AS 353 with the Subaru Telescope shows that it is a hierarchical triple system. The secondary component, located 5.6" south of AS 353A, is resolved into a subarcsecond binary, AS 353Ba and Bb, separated by 0.24". Resolved spectroscopy of the two close components shows that both have nearly identical spectral types of about M1.5. Whereas AS 353A and Ba show clear evidence for an infrared excess, AS 353Bb does not. We discuss the possible role of multiplicity in launching the large Herbig-Haro flow associated with AS 353A.Comment: AASTeXv5.0, 21 pages, 5 figures, Astronomical Journal, in pres

First Detection of NaI D lines in High-Redshift Damped Lyman-alpha Systems

A Near-infrared (1.18-1.35 micron) high-resolution spectrum of the gravitationally-lensed QSO APM 08279+5255 was obtained with the IRCS mounted on the Subaru Telescope using the AO system. We detected strong NaI D 5891,5897 doublet absorption in high-redshift DLAs at z=1.062 and 1.181, confirming the presence of NaI, which was first reported for the rest-frame UV NaI 3303.3,3303.9 doublet by Petitjean et al. This is the first detection of NaI D absorption in a high-redshift (z>1) DLA. In addition, we detected a new NaI component in the z=1.062 DLA and four new components in the z=1.181 DLA. Using an empirical relationship between NaI and HI column density, we found that all "components" have large HI column density, so that each component is classified as DLA absorption. We also detected strong NaI D absorption associated with a MgII system at z=1.173. Because no other metal absorption lines were detected in this system at the velocity of the NaI absorption in previously reported optical spectra (observed 3.6 years ago), we interpret this NaI absorption cloud probably appeared in the line of sight toward the QSO after the optical observation. This newly found cloud is likely to be a DLA based upon its large estimated HI column density. We found that the N(NaI)/N(CaII) ratios in these DLAs are systematically smaller than those observed in the Galaxy; they are more consistent with the ratios seen in the Large Magellanic Cloud. This is consistent with dust depletion generally being smaller in lower metallicity environments. However, all five clouds of the z=1.181 system have a high N(NaI)/N(CaII) ratio, which is characteristic of cold dense gas. We tentatively suggest that the host galaxy of this system may be the most significant contributor to the gravitational-lens toward APM 08279+5255.Comment: 22 pages, 6 Postscript figures, 3 tables, ApJ in press (Vol.643, 2 June 2006

Direct Observation of the Extended Molecular Atmosphere of o Cet by Differential Spectral Imaging with an Adaptive Optics System

We present new measurements of the diameter of o Cet (Mira) as a function of wavelength in the 2.2 micron atmospheric window using the adaptive optics system and the infrared camera and spectrograph mounted on the Subaru Telescope. We found that the angular size of the star at the wavelengths of CO and H2O absorption lines were up to twice as large as the continuum photosphere. This size difference is attributable to the optically thick CO and H2O molecular layers surrounding the photosphere. This measurement is the first direct differential spectroscopic imaging of stellar extension that resolves individual molecular lines with high spectral-resolution observations. This observation technique is extremely sensitive to differences in spatial profiles at different wavelengths; we show that a difference in diameter much smaller than the point spread function can be measured.Comment: 6 pages, 3 figures, accepted for publication in PAS