Overlap of the Rest binding sites between the ES and EpiS cells.

<p>Overlap of the Rest binding sites, associated with cording genes or non-coding genes, between the ES and EpiS cells, except for the R+/S−/L− sites. The numbers of genes associated with such sites are shown in parentheses.</p

Rest complex ChIP Seq.

<p>Examples of the Rest complex binding sites detected and associated with protein-coding genes in ES and EpiS cells. ChIP Seq tags for Rest, Sin3A, and Lsd1 in the indicated cells are shown. (A), (B), and (C) show examples of the ES-unique, common, and EpiS-unique sites in the vicinity of “Snap23”, “C2cd5 and Etnk1”, and “Cdh23”, respectively.</p

Transcription effects from Rest complex binding.

<p>(A and C) Boxplots of the transcript levels measured using TSS tag counts in ES (left lanes) or EpiS cells (right lanes) for the indicated category are shown. (B and D) The TSS tag count fold changes between the ES and EpiS cells for the indicated category are shown. The statistical significance for the differences was evaluated using Wilcoxon’s signed rank test, which is shown in the top margin.</p

Reprogramming efficiency of somatic cells in the presence of c-Myc.

<p>(A) Reprogramming efficiency of fibroblasts (MEF, NB fb, 1wk fb and Adult fb), hematopoietic cells (FL CD45, HSC, HPC, MP and Mac) by four reprogramming factors including <i>c-Myc</i>. (B) Cell proliferation rate of fibroblasts (MEF, NB fb, 1wk fb and Adult fb) at three, four and five days after seeded in the absence of Dox (left) and in the presence of Dox (right). (C) Reprogramming efficiency of MEF were compared between reprogrammed by three factors (Oct4, Klf4 and Sox2), four factors (<i>Oct4</i>, <i>Klf4</i>, <i>Sox2</i> and <i>c-Myc</i>) and three factors plus VPA (0.5 mM, 1 mM and 2 mM).</p

Construction of Dox inducible reprogramming system.

<p>(A) Schematic diagram of Dox inducible system for expression of reprogramming factors. (B) Alkaline phosphatase (AP) staining of iPS colonies derived from Ai-LV transduced MEFs (left panel). Efficiency of AP positive colonies (right panel). Efficiency of AP positive colonies calculated by dividing infected cell number by the number of AP positive colonies. (B) RT-PCR analysis of endogenous pluripotent marker genes, with or without Dox in the culture. (C) Immunofluorescence staining for <i>Nanog</i> in iPS clone#6, with or without Dox in the culture.</p