Evolution of Soil Desaturation by Air-Injection Technique and Its Evaluation via Multiphase Flow Simulation

In this study the simplified model tests that simulate air injection into saturated soils using air-injection probes, are conducted using the two different sizes of soil containers. The experiments using the small container are aimed to examine the rates and magnitudes of the soil desaturation driven by air injection, whilst those with the large container are performed to obtain not only the rates and magnitudes but also the distributions of the desaturated zones within the soil, and to examine influences on the desaturation process exerted by anisotropy of the soil in terms of flow transport. Obtained results indicate that the evolution of desaturation is strongly controlled by the soil permeabilities. Numerical analyses are also conducted using a multiphase flow simulator to describe the evolution of the soil desaturation, and to examine an applicability of the model as a prediction tool enabling an evolution of desaturation in situ to be followed with time and space. Predictions show a relatively good agreement with the measurements regarding the rates, magnitudes, and distribution of desaturation although predictions of the airflow rates underestimate the measurements for both small- and large-container experiments, and in the large container the early periods of the experiments are ill-replicated by the model

New Mastoparan Peptides in the Venom of the Solitary Eumenine Wasp Eumenes micado

Comprehensive LC-MS and MS/MS analysis of the crude venom extract from the solitary eumenine wasp Eumenes micado revealed the component profile of this venom mostly consisted of small peptides. The major peptide components, eumenine mastoparan-EM1 (EMP-EM1: LKLMGIVKKVLGAL-NH2) and eumenine mastoparan-EM2 (EMP-EM2: LKLLGIVKKVLGAI-NH2), were purified and characterized by the conventional method. The sequences of these new peptides are homologous to mastoparans, the mast cell degranulating peptides from social wasp venoms; they are 14 amino acid residues in length, rich in hydrophobic and basic amino acids, and C-terminal amidated. Accordingly, these new peptides can belong to mastoparan peptides (in other words, linear cationic α-helical peptides). Indeed, the CD spectra of these new peptides showed predominantly α-helix conformation in TFE and SDS. In biological evaluation, both peptides exhibited potent antibacterial activity, moderate degranulation activity from rat peritoneal mast cells, and significant leishmanicidal activity, while they showed virtually no hemolytic activity on human or mouse erythrocytes. These results indicated that EMP-EM peptides rather strongly associated with bacterial cell membranes rather than mammalian cell membranes

Eumenitin, a novel antimicrobial peptide from the venom of the solitary eumenine wasp Eumenes rubronotatus

A novel antimicrobial peptide, eumenitin, was isolated from the venom of the solitary eumenine wasp Eumenes rubronotatus. The sequence of eumenitin, Leu-Asn-Leu-Lys-Gly-Ile-Phe-Lys-Lys-Val-Ala-Ser-Leu-Leu-Thr, was mostly analyzed by mass spectrometry together with Edman degradation, and corroborated by solid-phase synthesis. This peptide has characteristic features of cationic linear a-helical antimicrobial peptides, and therefore, can be predicted to adopt an amphipathic a-helix secondary structure. In fact, the CD spectra of eumenitin in the presence of TFE or SDS showed a high content of alpha-helical conformation. Eumenitin exhibited inhibitory activity against both Gram-positive and Gram-negative bacteria, and moderately stimulated degranulation from the rat peritoneal mast cells and the RBL-2H3 cells, but showed no hemolytic activity against human erythrocytes. This antimicrobial peptide in the eumenine wasp venom may play a role in preventing potential infection by microorganisms during prey consumption by their larvae. (c) 2006 Elsevier B.V. All rights reserved