Depositional environments of the lower Tullock Formation (Paleocene), Slope County, southwestern North Dakota

The lower Tullock Formation (Paleocene), which consists of a section 5 to 30 feet thick, was studied in Slope County, southwestern North Dakota to determine its depositional environments. The lithol ogies present are sand, silt, clay and lignite. A bed of lignite or yellow silt marks the base of the Tullock Formation throughout the study area. The silt beds are interpreted as having been deposited as natural levees because they are wedge-shaped and contain climbing ripple cross-stratification, interbedded sand lenses and lignitized paleo-raot systems. Well-drained swamp deposits are difficult to distinguish, but are probably located in the silt beds. There are two types of clay beds which were deposited in different envirorunents. The first type of clay bed is dark-colored, rich in organic matter, silty and shaly. It is interpreted as having been deposited in a poorly-drained swamp in a fluvial floodbasin because of its organic content and interbedded relationships with lignite beds. The other type of clay bed is light-colored and contains less organic material and silt than the first type. It is interpreted as having been deposited in small lakes within a floodbasin because of its low organic content, massively bedded clay and stratigraphic position above lignite beds. Lignite beds are interpreted as having been deposited in poorly drained swamps in fluyial floodbasins because of their wood content, lateral persistence, thickness and interbedded relationships with dark colored clay beds. The sand beds of the lower Tullock Formation are interpreted as having been deposited as crevasse-splays because their grain size (very fine- to medium-grained) is coarser than levee deposits, and they show lateral thinning, scoured bases and small-scale ripple cross stratification. The sand beds are underlain by lignite and overlain by dark-colored clay indicating the sand was rapidly dispersed into poorly-drained swamps. Prodelta and near- and off-shore lacustrine environments of deposition are rejected because of incorrect vertical successions of beds. Commonly clay beds rich in organic matter are overlain by lignite beds, which, in turn, are overlain by silt beds. Lacustrine deposits would show clay beds overlain by silt and sand beds, in turn overlain by lignite beds. The succession present is what would be expected for a fluvial environment

Consistency in Patients' Health and Treatment Expectations at a Geriatric Clinic

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/111146/1/j.1532-5415.1981.tb02192.x.pd

A Brief Life‐Graph Technique for Work with Geriatric Patients*

Patients aged 60 or older from the practice of a private physician (n = 32) and from a geriatric outpatient clinic (n = 132) responded to a questionnaire designed to assess perceived present and future health, treatment expectations, and general future projection. Of interest was the extent to which present health, as measured by a brief life‐graph technique, might be predictive of perceptions in these other areas. Results from the two samples were consistent in suggesting that present health ratings were related to anticipated future health, general future projection, and certain treatment expectations. However, expectations of when benefits from treatment would begin, and of the probable duration of treatment, were not predicted in either sample. The life‐graph technique seems useful for practitioners' interactions with older patients and for understanding these patients' extended views of their health.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/111234/1/jgs01244.pd

The phn Island: A New Genomic Island Encoding Catabolism of Polynuclear Aromatic Hydrocarbons

Bacteria are key in the biodegradation of polycyclic aromatic hydrocarbons (PAH), which are widespread environmental pollutants. At least six genotypes of PAH degraders are distinguishable via phylogenies of the ring-hydroxylating dioxygenase (RHD) that initiates bacterial PAH metabolism. A given RHD genotype can be possessed by a variety of bacterial genera, suggesting horizontal gene transfer (HGT) is an important process for dissemination of PAH-degrading genes. But, mechanisms of HGT for most RHD genotypes are unknown. Here, we report in silico and functional analyses of the phenanthrene-degrading bacterium Delftia sp. Cs1-4, a representative of the phnAFK2 RHD group. The phnAFK2 genotype predominates PAH degrader communities in some soils and sediments, but, until now, their genomic biology has not been explored. In the present study, genes for the entire phenanthrene catabolic pathway were discovered on a novel ca. 232 kb genomic island (GEI), now termed the phn island. This GEI had characteristics of an integrative and conjugative element with a mobilization/stabilization system similar to that of SXT/R391-type GEI. But, it could not be grouped with any known GEI, and was the first member of a new GEI class. The island also carried genes predicted to encode: synthesis of quorum sensing signal molecules, fatty acid/polyhydroxyalkanoate biosynthesis, a type IV secretory system, a PRTRC system, DNA mobilization functions and >50 hypothetical proteins. The 50% G + C content of the phn gene cluster differed significantly from the 66.7% G + C level of the island as a whole and the strain Cs1-4 chromosome, indicating a divergent phylogenetic origin for the phn genes. Collectively, these studies added new insights into the genetic elements affecting the PAH biodegradation capacity of microbial communities specifically, and the potential vehicles of HGT in general

Progressive changes in microglia and macrophages in spinal cord and peripheral nerve in the transgenic rat model of amyotrophic lateral sclerosis

<p>Abstract</p> <p>Background</p> <p>The role of neuroinflammation in motor neuron death of amyotrophic lateral sclerosis (ALS) is unclear. The human mutant superoxide dismutase-1 (hmSOD1)-expressing murine transgenic model of ALS has provided some insight into changes in microglia activity during disease progression. The purpose of this study was to gain further knowledge by characterizing the immunological changes during disease progression in the spinal cord and peripheral nerve using the more recently developed hmSOD1 rat transgenic model of ALS.</p> <p>Methods</p> <p>Using immunohistochemistry, the extent and intensity of tissue CD11b expression in spinal cord, lumbar nerve roots, and sciatic nerve were evaluated in hmSOD1 rats that were pre-clinical, at clinical onset, and near disease end-stage. Changes in CD11b expression were compared to the detection of MHC class II and CD68 microglial activation markers in the ventral horn of the spinal cord, as well as to the changes in astrocytic GFAP expression.</p> <p>Results</p> <p>Our study reveals an accumulation of microglia/macrophages both in the spinal cord and peripheral nerve prior to clinical onset based on CD11b tissue expression. The microglia formed focal aggregates in the ventral horn and became more widespread as the disease progressed. Hypertrophic astrocytes were not prominent in the ventral horn until after clinical onset, and the enhancement of GFAP did not have a strong correlation to increased CD11b expression. Detection of MHC class II and CD68 expression was found in the ventral horn only after clinical onset. The macrophages in the ventral nerve root and sciatic nerve of hmSOD1 rats were observed encircling axons.</p> <p>Conclusions</p> <p>These findings describe for the first time in the hmSOD1 rat transgenic model of ALS that enhancement of microglia/macrophage activity occurs pre-clinically both in the peripheral nerve and in the spinal cord. CD11b expression is shown to be a superior indicator for early immunological changes compared to other microglia activation markers and astrogliosis. Furthermore, we suggest that the early activity of microglia/macrophages is involved in the early phase of motor neuron degeneration and propose that studies involving immunomodulation in hmSOD1transgenic models need to consider effects on macrophages in peripheral nerves as well as to microglia in the spinal cord.</p

New Era Begins: Mental Health Law Reform in Virginia

Amidst calls for mental health reform and a sense of urgency stemming from the tragic events at Virginia Tech, the 2008 session of the Virginia General Assembly convened. The legislative reaction was overwhelming: Legislators introduced a vast array of bills relating to mental health. By the end of the session, the General Assembly enacted the most sweeping revisions to Virginia\u27s mental health laws since the 1970s

Strain-dependent variation in the early transcriptional response to CNS injury using a cortical explant system

<p>Abstract</p> <p>Background</p> <p>While it is clear that inbred strains of mice have variations in immunological responsiveness, the influence of genetic background following tissue damage in the central nervous system is not fully understood. A cortical explant system was employed as a model for injury to determine whether the immediate transcriptional response to tissue resection revealed differences among three mouse strains.</p> <p>Methods</p> <p>Immunological mRNAs were measured in cerebral cortex from SJL/J, C57BL/6J, and BALB/cJ mice using real time RT-PCR. Freshly isolated cortical tissue and cortical sections incubated in explant medium were examined. Levels of mRNA, normalized to β-actin, were compared using one way analysis of variance with pooled samples from each mouse strain.</p> <p>Results</p> <p>In freshly isolated cerebral cortex, transcript levels of many pro-inflammatory mediators were not significantly different among the strains or too low for comparison. Constitutive, baseline amounts of CD74 and antisecretory factor (ASF) mRNAs, however, were higher in SJL/J and C57BL/6J, respectively. When sections of cortical tissue were incubated in explant medium, increased message for a number of pro-inflammatory cytokines and chemokines occurred within five hours. Message for chemokines, IL-1α, and COX-2 transcripts were higher in C57BL/6J cortical explants relative to SJL/J and BALB/cJ. IL-1β, IL-12/23 p40, and TNF-α were lower in BALB/cJ explants relative to SJL/J and C57BL/6J. Similar to observations in freshly isolated cortex, CD74 mRNA remained higher in SJL/J explants. The ASF mRNA in SJL/J explants, however, was now lower than levels in both C57BL/6J and BALB/cJ explants.</p> <p>Conclusions</p> <p>The short-term cortical explant model employed in this study provides a basic approach to evaluate an early transcriptional response to neurological damage, and can identify expression differences in genes that are influenced by genetic background.</p