Grapevine shoot extract rich in trans-resveratrol and trans-ε-viniferin: evaluation of their potential use for cardiac health

A grapevine shoot extract (GSE) was obtained using ultrasound-assisted extraction and characterized. The main phenolic constituents were identified as stilbenoids. Among them, trans-resveratrol and trans-ε-viniferin stood out. The GSE was administered to an isoproterenol-induced myocardial injury animal model. The extract alleviated the associated symptoms of the administration of the drug, i.e., the plasma lipid profile was improved, while the disturbed plasma ion concentration, the cardiac dysfunction markers, the DNA laddering, and the necrosis of myocardial tissue were diminished. This effect could be related to the anti-oxidative potential of GSE associated with its antioxidant properties, the increased levels of endogenous antioxidants (glutathione and enzymatic antioxidants), and the diminished lipid peroxidative markers in the heart. The results also revealed angiotensin-converting enzyme (ACE)-inhibitory activity, which indicated the potential of GSE to deal with cardiovascular disease events. This work suggests that not only trans-resveratrol has a protective role in heart function but also GSE containing this biomolecule and derivatives. Therefore, GSE has the potential to be utilized in the creation of innovative functional ingredients.Ministry of Science and Innovation (MCIN) of Spain for the Ramón y Cajal grant (RYC2020-030546-I/AEI/10.13039/501100011033). European Social Fund. “Programme of Requalification of the Spanish University System” 2021–2023—Margarita Salas. MCIN/AEI/10.13039/501100011033, grant PID2020-112594RB-C31, “ERDF A way of making Europe”. “Researchers Supporting Project (RSP2023R17) at King Saud University

A fully coupled damage model with stress triaxiality and Lode dependence

International audienceAbstract In the present work, an advanced CDM model considering stress triaxiality and Lode angle effect is proposed. The framework of thermodynamics of irreversible processes with state variables is used to build the constitutive equations accounting for strong and full coupling between all the dissipative phenomena. The proposed model is implemented into Finite Element (FE) code ABAQUS/Explicit via a user material subroutine (VUMAT). A detailed parametric study with various values of the new material parameters is conducted in order to show the predictive capability of the proposed model. Applications to sheet metal forming simulation have been performed to validate the damage prediction capability of the proposed model, and the numerical simulation results are analysed and discussed

Impact of dietary restriction on peroxidative effects of nickel chloride in wistar rats

International audienceThe purpose of this study, carried out in Wistar rats, was to evaluate the protective effect of dietary restriction (performed by intermittent fasting) against oxidative stress induced by a low concentration of nickel chloride in kidney, liver, uterus, and ovary. Lipid peroxidation (TBARS), catalase activity, and the levels of vitamins E and A in the blood were investigated in rats feed for 1 month either daily (N) or 1 day over two (intermittent fasting, IF) and then injected (NNi, IFNi) or not with nickel chloride (30 moles/kg body weight/day) for 10 days. Ni induced a significant increase of TBARS in organs of N rats. Intermittent fasting alone or associated to nickel treatment did not result in TBARS change in IF and IFNi rats. Catalase activity levels were found to be similar in N and IF rats. In Ni-treated rats a transient increase of catalase activity appeared at day 1 in the kidney and days 1 and 3 in the liver. Then, catalase activity was found to be inhibited until day 10. In the uterus and ovary, catalase activity was always found to be inhibited. In IFNi rats, no significant increase of catalase activity was observed as compared to IF rats. Vitamin E was inhibited from the 1st to the 10th day in Ni rats, whereas no significant changes were noted in IFNi rats. A moderate decrease of vitamin A was only found at days 1 and 3 in Ni rats. In conclusion, intermittent fasting is able to protect from oxidative stress induced by low concentration of Ni, but catalase and Vitamins E and A do not seem to be involved