The dynamics of phase partition. A study of parameters affecting rat liver organelle partitioning in aqueous two-polymer phase systems

Separation of subcellular organelles by two-phase partition is thought to reflect differential partition of the organelles between the two phases or between one of the phases and the interface. Studies by Fisher and colleagues [Fisher & Walter (1984) Biochim. Biophys. Acta 801, 106-110] suggest that cell separation by phase partition is a dynamic process in which the partition changes with time. This is mainly due to association of the cells with sedimenting droplets of one phase in the bulk of the other. Rat liver organelle partition was studied to determine whether the same dynamic behaviour is observed. Partition was clearly time-dependent during 24 h at unit gravity, and was also affected by altering the volume ratio of the two phases and the duration of phase mixing. These results indicate that, as with cells, the partition of organelles between phases is a dynamic process, and is consistent with the demonstration that organelles adhere to the phase droplet surfaces. Optimization of the volume ratio between phases may lead to significant processing economies. Organelle sedimentation in the upper phase was significantly faster than in the isoosmotic sucrose. Theoretical modelling of apparent organelle sizes indicates that aggregation occurs in the poly(ethylene glycol)-rich upper phase. This phenomenon is likely to limit the use of this technique in organelle separations unless means can be found to decrease aggregation

Evolution of faint radio sources in the VIDEO-XMM3 field

© 2013 The Authors Published by Oxford University Press on behalf of the Royal Astronomical SocietyIt has been speculated that low-luminosity radio-loud active galactic nuclei (AGN) have the potential to serve as an important source of AGN feedback, and may be responsible for suppressing star formation activity in massive elliptical galaxies at late times. As such the cosmic evolution of these sources is vitally important to understand the significance of such AGN feedback processes and their influence on the global star formation history of the Universe. In this paper, we present a new investigation of the evolution of faint radio sources out to z ~ 2.5. We combine a 1 square degree Very Large Array radio survey, complete to a depth of 100 μJy, with accurate 10 band photometric redshifts from the following surveys: Visible and Infrared Survey Telescope for Astronomy Deep Extragalactic Observations and Canada-France-Hawaii Telescope Legacy Survey. The results indicate that the radio population experiences mild positive evolution out to z ~ 1.2 increasing their space density by a factor of ~3, consistent with results of several previous studies. Beyond z = 1.2, there is evidence of a slowing down of this evolution. Star-forming galaxies drive the more rapid evolution at low redshifts, z 1.2. The evolution is best fitted by pure luminosity evolution with star-forming galaxies evolving as (1 + z)2.47 ± 0.12 and AGN as (1 + z)1.18 ± 0.21M.Peer reviewe