La validité et la fiabilité du test du jugement de la reconnaissance de la latéralité: une revue systématique

La reconnaissance de la latéralité est une capacité implicite de notre cerveau à différencier la partie du corps droite de la gauche. Elle est évaluée par le programme Recognise qui propose des images de divers membres orientés à différents degrés et dont le sujet doit indiquer la latéralité. Notre objectif est une revue de la littérature pour définir si ce test est valide et fiable.Laterality recognition is an implicit capability of our brain to differentiate between the left and right side of the body. It is evaluated by the Recognise program which offers a display of body part images oriented in various degrees. The subject has to indicate the laterality of the limb. The aim of our literature review is to establish if the test of laterality recognition is valid and reliable.Die Lateralitätserkennung ist eine implizite Fähigkeit unseres Gehirns, die linke von der rechten Körperhälfte zu unterscheiden. Sie wird durch das Programm Recognise bestimmt, das verschiedene Bilder von Gliedmassen in unterschiedlichen Stellungswinkeln zeigt. Der Teilnehmer soll die Lateralität der Gliedmassen erkennen. Das Ziel unserer Bachelorarbeit ist eine systematische Durchsicht der einschlägigen Fachliteratur, um zu bestimmen, ob der Lateralitätserkennungstest stichhaltig und verlässlich ist

Thalamic activity that drives visual cortical plasticity

Manipulations of activity in one retina can profoundly affect binocular connections in the visual cortex. Retinal activity is relayed to the cortex by the dorsal lateral geniculate nucleus (dLGN). We compared the qualities and amount of activity in the dLGN following monocular eyelid closure and monocular retinal inactivation in awake mice. Our findings substantially alter the interpretation of previous studies and define the afferent activity patterns that trigger cortical plasticity.National Eye InstituteNational Institute of Neurological Disorders and Stroke (U.S.) (National Research Service Award fellowship

Decreased glutathione biosynthesis contributes to EGFR T790M-driven erlotinib resistance in non-small cell lung cancer

Epidermal growth factor receptor (EGFR) inhibitors such as erlotinib are novel effective agents in the treatment of EGFR-driven lung cancer, but their clinical impact is often impaired by acquired drug resistance through the secondary T790M EGFR mutation. To overcome this problem, we analysed the metabonomic differences between two independent pairs of erlotinib-sensitive/resistant cells and discovered that glutathione (GSH) levels were significantly reduced in T790M EGFR cells. We also found that increasing GSH levels in erlotinib-resistant cells re-sensitised them, whereas reducing GSH levels in erlotinib-sensitive cells made them resistant. Decreased transcription of the GSH-synthesising enzymes (GCLC and GSS) due to the inhibition of NRF2 was responsible for low GSH levels in resistant cells that was directly linked to the T790M mutation. T790M EGFR clinical samples also showed decreased expression of these key enzymes; increasing intra-tumoural GSH levels with a small-molecule GST inhibitor re-sensitised resistant tumours to erlotinib in mice. Thus, we identified a new resistance pathway controlled by EGFR T790M and a therapeutic strategy to tackle this problem in the clinic

Megapolitan Political Ecology and Urban Metabolism in Southern Appalachia

Drawing on megapolitan geographies, urban political ecology, and urban metabolism as theoretical frameworks, this article theoretically and empirically explores megapolitan political ecology. First, we elucidate a theoretical framework in the context of southern Appalachia and, in particular, the Piedmont megapolitan region, suggesting that the megapolitan region is a useful scale through which to understand urban metabolic connections that constitute this rapidly urbanizing area. We also push the environmental history and geography literature of the U.S. South and southern Appalachia to consider the central role urban metabolic connections play in the region's pressing social and environmental crises. Second, we empirically illuminate these human and nonhuman urban metabolisms across the Piedmont megapolitan region using data from the Coweeta Long-Term Ecological Research (LTER) program, especially highlighting a growing “ring of asphalt” that epitomizes several developing changes to patterns of metabolism. The conclusion suggests that changing urban metabolisms indicated by Coweeta LTER data, ranging from flows of people to flows of water, pose a complicated problem for regional governance and vitality in the future

Air temperature variability in a high-elevation Himalayan catchment

Air temperature is a key control of processes affecting snow and glaciers in high-elevation catchments, including melt, snowfall and sublimation. It is therefore a key input variable to models of land–surface–atmosphere interaction. Despite this importance, its spatial variability is poorly understood and simple assumptions are made to extrapolate it from point observations to the catchment scale. We use a dataset of 2.75 years of air temperature measurements (from May 2012 to November 2014) at a network of up to 27 locations in the Langtang River, Nepal, catchment to investigate air temperature seasonality and consistency between years. We use observations from high elevations and from the easternmost section of the basin to corroborate previous findings of shallow lapse rates. Seasonal variability is strong, with shallowest lapse rates during the monsoon season. Diurnal variability is also strong and should be taken into account since processes such as melt have a pronounced diurnal variability. Use of seasonal lapse rates seems crucial for glacio-hydrological modelling, but seasonal lapse rates seem stable over the 2–3 years investigated. Lateral variability at transects across valley is high and dominated by aspect, with south-facing sites being warmer than north-facing sites and deviations from the fitted lapse rates of up to several degrees. Local factors (e.g. topographic shading) can reduce or enhance this effect. The interplay of radiation, aspect and elevation should be further investigated with high-elevation transects

Quantification of MUCIN 1, cell surface associated and MUCIN16, cell surface associated proteins in tears and conjunctival epithelial cells collected from postmenopausal women

Srinivasan, S., Heynen, M. L., Martell, E., Ritter, R., Jones, L., & Senchyna, M. (2013). Quantification of MUCIN 1, cell surface associated and MUCIN16, cell surface associated proteins in tears and conjunctival epithelial cells collected from postmenopausal women. Molecular Vision, 19, 970–979.Purpose: To quantify the expression of mucin 1, cell surface associated (MUC1) and mucin 16, cell surface associated (MUC16) proteins and messenger ribonucleic acid (mRNA) in a cohort of postmenopausal women (PMW), to explore the relationship between mucin expression, dry eye symptomology, and tear stability.Methods: Thirty-nine healthy PMW (>50 years of age) were enrolled in this study. No specific inclusion criteria were used to define dry eye; instead, a range of subjects were recruited based on responses to the Allergan Ocular Surface Disease Index (OSDI) questionnaire and tear stability measurements as assessed by non-invasive tear breakup time (NITBUT). Tears were collected from the inferior tear meniscus using a disposable glass capillary tube, and total RNA and total protein were isolated from conjunctival epithelial cells collected via impression cytology. Expression of membrane-bound and soluble MUC1 and MUC16 were quantified with western blotting, and expression of MUC1 and MUC16 mRNA was assessed with real-time PCR.Results: OSDI responses ranged from 0 to 60, and NITBUT ranged from 18.5 to 2.9 s. Only two statistically significant correlations were found: soluble MUC16 protein concentration and MUC16 mRNA expression with OSDI vision related (−0.47; p=0.01) and ocular symptom (0.39; p=0.02) subscores, respectively. Post hoc exploratory analysis on absolute expression values was performed on two subsets of subjects defined as asymptomatic (OSDI ≤6, n=12) and moderate to severe symptomatic (OSDI ≥20, n=12). The only significant difference between the two subgroups was a significant reduction in MUC16 mRNA expression found in the symptomatic dry eye group (1.52±1.19 versus 0.57±0.44; p=0.03).Conclusions: A broad exploration of mucin expression compared to either a sign (NITBUT) or symptoms of dry eye failed to reveal compelling evidence supporting a significant relationship, other than a potential association between MUC16 with specific symptoms. Furthermore, comparison of mucin protein and expression levels between the asymptomatic and moderate to severe symptomatic subgroups revealed only one significant difference, a reduction in MUC16 mRNA expression in the symptomatic subgroup.Funding for this study was provided by Alcon Research Ltd and the Natural Sciences and Engineering Research Council of Canada (NSERC)

Can science writing collectives overcome barriers to more democratic communication and collaboration? Lessons from environmental communication praxis in southern Appalachia

Despite compelling reasons to involve nonscientists in the production of ecological knowledge, cultural and institutional factors often dis-incentivize engagement between scientists and nonscientists. This paper details our efforts to develop a biweekly newspaper column to increase communication between ecological scientists, social scientists, and the communities within which they work. Addressing community-generated topics and written by a collective of social and natural scientists, the column is meant to foster public dialog about socio-environmental issues and to lay the groundwork for the coproduction of environmental knowledge. Our collective approach to writing addresses some major barriers to public engagement by scientists, but the need to insert ourselves as intermediaries limits these gains. Overall, our efforts at environmental communication praxis have not generated significant public debate, but they have supported future coproduction by making scientists a more visible presence in the community and providing easy pathways for them to begin engaging the public. Finally, this research highlights an underappreciated barrier to public engagement: scientists are not merely disconnected from the public, but also connected in ways that may be functional for their research. Many field scientists, for example, seek out neutral and narrowly defined connections that permit research access but are largely incompatible with efforts to address controversial issues of environmental governance

Towards a synthesized critique of neoliberal biodiversity conservation

During the last three decades, the arena of biodiversity conservation has largely aligned itself with the globally dominant political ideology of neoliberalism and associated governmentalities. Schemes such as payments for ecological services are promoted to reach the multiple ‘wins’ so desired: improved biodiversity conservation, economic development, (international) cooperation and poverty alleviation, amongst others. While critical scholarship with respect to understanding the linkages between neoliberalism, capitalism and the environment has a long tradition, a synthesized critique of neoliberal conservation - the ideology (and related practices) that the salvation of nature requires capitalist expansion - remains lacking. This paper aims to provide such a critique. We commence with the assertion that there has been a conflation between ‘economics’ and neoliberal ideology in conservation thinking and implementation. As a result, we argue, it becomes easier to distinguish the main problems that neoliberal win-win models pose for biodiversity conservation. These are framed around three points: the stimulation of contradictions; appropriation and misrepresentation and the disciplining of dissent. Inspired by Bruno Latour’s recent ‘compositionist manifesto’, the conclusion outlines some ideas for moving beyond critique

Considerations and guidelines for import and export of ornithological samples from tropical countries

„Biodiversität“ wird zunehmend als wichtige Ressource erkannt. Schutz, Zugang und nachhaltige Nutzung der Biodiversität (genetische Ressourcen, Arten, Proben jeglicher Couleur) werden inzwischen auf verschiedenen politischen Ebenen verhandelt, was in international verbindlichen Rahmenwerken verankert wird. Verständnis von und Bewusstsein über Genehmigungen zum Import und Export biologischer Proben ist von zunehmender Bedeutung für Biologen, um Forschungsprojekte legal und zeitnah durchführen zu können. Nichtsdestotrotz werden nach wie vor biologische Proben fleißig im- und exportiert, oft genug auch unter Vernachlässigung der Genehmigungspflicht aufgrund lokaler, nationaler und internationaler Übereinkommen, Gesetzen und Verordnungen bzw. auch schlichtweg mit gesetzeswidrigem Verhalten oder Verpackungen beim Transport. Daraus entstehende Schwierigkeiten können ernsthafte Probleme während der Feldarbeit bedeuten, aber auch den Export verzögern oder zum Verlust von Proben führen. Intensive rechtzeitige (Vorab-) Information bezüglich gesetzlicher Voraussetzungen zum Import, Export und Transport biologischer Proben kann das Problempotenzial stark vermindern oder ganz beseitigen. Wir haben vier wichtige Faktoren identifiziert und fassen Informationen zu diesen Bereichen zusammen, die bei Beachtung die Genehmigungen und den Import in die EG vereinfachen können: (1) gute persönliche (auf gegenseitigem Vertrauen beruhende) Kontakte im Ursprungsland; (2) Verständnis und Einhaltung von allen relevanten Gesetzen und Verordnungen; (3) Zugang zu Informationen bezüglich Genehmigungen, Verordnungen und Informationsverbreitung innerhalb der Forschergemeinschaft; und (4) Zugang zu einheitlichen und aktuellen Richtlinien zu Genehmigungen, Verordnungen und Gesetzen. Ziel dieser Arbeit ist es, in Zukunft die Forschung von einigen zentralen Problemen im Im- und Export zu befreien und Probleme und Missverständnisse zu reduzieren.‘Biodiversity’ is increasingly perceived as an important resource. Conservation, access and sustainable use of biodiversity (genetic resources, species, samples) are negotiated at political levels, resulting in an internationally binding legal framework. Understanding and awareness of export and import permits for biological samples is increasingly important for biologists to perform research projects legally and timely. Nevertheless, some biologists still export and import biological samples in disregard of or non-compliant with national and international legislation, conventions, and regulations. Resulting difficulties may not only cause serious problems during field work, but may also delay the export, import or exchange of samples. Comprehensive a priori information regarding legal requirements helps to avoid or at least diminish potential problems. We identified four major factors facilitating export/import permits: (1) good personal (mutually trusted) contacts in the country of origin, (2) understanding and compliance with all relevant laws and regulations; (3) access to information regarding knowledge on permits, regulations and laws including their circulation within the researcher communities and (4) access to consistent and up to date regulations and guidelines. We provide information on key issues to assist research teams trying to reduce problems and misunderstandings